BBa_K1641000
1
BBa_K1641000
Recombinase Vcre, E. coli-and-yeast coding optimized
2015-09-07T11:00:00Z
2015-09-08T01:59:04Z
This recombinase comes from de novo gene synthesis.
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system. Vcre has the invertase activity that invert the the sequence between two anti-parallel Vloxp.
The original Vcre is discovered through genome screening of Vibrio sp. 0908, yet this coding sequence is redesigned into E. coli-and-yeast coding preference from original protein sequence.
false
false
_2058_
20036
20036
9
false
Optimized for both E. coli and S. cerevisiae, but neither is most perfectly fitted.
false
Pai Li
annotation2446676
1
Vcre coding
range2446676
1
1
1146
BBa_K1641000_sequence
1
atgattgaaaatcaactgtccctgctgggtgatttttcgggcgttcgtcctgatgacgtgaaaacagccattcaagctgcacagaaaaagggtatcaatgttgcggaaaacgaacagtttaaagccgcgttcgaacatttgctgaacgaatttaaaaagagagaagaacgctactccccaaacaccttgcgtagactggaatcggcctggacttgtttcgttgactggtgcttagccaatcatagacacagcttgcctgcgaccccagatactgtggaagcatttttcattgaacgcgcagaagaactgcatcgtaacacattaagcgtctatagatgggctatcagtcgtgttcacagagtggcaggttgtccagatccgtgcctggacatttacgtggaagatcgcttaaaagccatcgcgagaaaaaaggtccgcgaaggcgaagccgtaaagcaagcgagtccatttaacgaacagcatttattgaaactgacgtccttgtggtatcgctcggataagctgttattgcgccgtaatctggcgctgttagctgtcgcatacgaatctatgttgagagcttcagaactggcaaacattcgcgtatctgatatggaattggccggtgacggcacagcgatcctgacgattccgatcacaaaaacgaatcactcaggtgaacctgatacatgtattctgagccaagacgttgtgagtttgctgatggattatacggaagctggcaaactggatatgtcttcagacggttttctgttcgtcggcgtttctaagcataacacatgcatcaagccaaaaaaggataagcaaacgggtgaagttctgcacaaaccgatcaccactaagaccgtcgaaggcgtattttacagtgcatgggaaactctggacttaggtcgtcagggcgttaaaccgttcaccgctcattccgcaagagtgggtgctgcacaagatttattgaaaaagggctacaacactctgcaaattcaacagtcaggtcgctggagcagtggcgctatggttgcgcgctacggtcgtgctatcttagcacgtgatggcgctatggctcactctcgtgttaaaactagatcagctcctatgcagtggggtaaagatgaaaaggactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z