BBa_K1641001 1 BBa_K1641001 Recombinase Scre, E. coli and yeast coding optimized 2015-09-07T11:00:00Z 2015-09-08T02:14:19Z It comes from de novo synthesis of DNA. This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence SloxP (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. However, because the wild type SloxP contains consecutive ATG that takes risk to unexpectedly initiate protein translation, we highly recommend to use an derivative of SloxP, SloxM1 as the target of Scre. Scre has the invertase activity that invert the the sequence between two anti-parallel SloxM1. The original Scre is discovered through genome screening of Shewanella sp. ANA-3, yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to its AA sequence. false false _2058_ 20036 20036 9 false Optimized for both E. coli and yeast. false Pai Li annotation2446617 1 Scre coding range2446617 1 1 1119 BBa_K1641001_sequence 1 atgtccctgttaaccactaataaccattcagtggctctgagctatggtgaaccaccgagcaccttgaatgatagtctgaaagactcctaccaaagatcgactgatgaactgcaagctctgctgtctaaaccgttagcccaattgacagatgcggacaagttacgtattcgtgaaatcacgcaggcaaaactgaagcatttcttggacaatggccaccgcacacgtagagctaacacgtggcgcgcattaatgtctcgttgggccaaatttgaatcatggtgtttgaccaataacctgactcctttaccagctacccctgaagttgtggcaactttcattgaatattaccaagcgtcttcatatacaacgctgagccagtacgcttgggcaatcaatagttttcatgttgaatgcggtttattgtcacctgtcagcagtaaaacagtacaagataagcagaacgaaattcgtatcgtgaaattggaatctggtggcctggctcaagaacaggcaactccattcagattgcatcacctgcaaatgttaattgaatcttatggcgaatcagaaagactgttagataaacgcaatctggccttgctgaacattgcgtacgaatccttattgcgtgaatcggaactgttaagaatcaaagtcggtcacttgaagtccacctttgaaggcgattatgtcctgtctgttccatacaccaaaactaatgactccggtgaagaagaagtcgtaaacattacaccgctgggctttaaactgattcaacgctatatccagggtgctggcttaacgaaggaagattacttgttccaaccgatcggtcgtagcaataaagttagtgtgcaggccaagcctatgagcacacgtacggttgatagagtgtttctgtgggcgttcgaaagtttaggcattgacagacattccgcatggtcgggtcactctgcacgtatcggtgctgcacaggatttgctggccgcgggttattctattgcccaaatccaggaaaacggccgctggaaatcaccaatgatggttctgcgttatggcaaggacattaaagctaaggaatcagctatggctaaaatgctggcagaacgccgttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z