BBa_K1641003
1
BBa_K1641003
Recombinase Vika, E. coli and yeast coding optimized
2015-09-07T11:00:00Z
2015-09-08T03:42:02Z
De novo synthesis.
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system.
Vika has the invertase activity that invert the the sequence between two anti-parallel Voxs. The original Vika is discovered through genome screening of Vibrio coralliilyticus ATCC BAA-450, which might be a integrase gene taken in by some phage. Yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to original protein sequence.
false
false
_2058_
20036
20036
9
false
This recombinase is both E. coli and S. cerevisiae coding optimized, but neither is perfectly fitted.
false
Pai Li
annotation2446620
1
Vika coding
range2446620
1
1
1089
BBa_K1641003_sequence
1
atgaccgatctgactccgtttccaccgttggaacatctggaacctgatgaatttgccgacttagtcagaaaagcgattaagcgcgatccacaagccggtgcgcaccctgcaattcaaagcgcgatcagtcattttcaggacgaatttgtacgtagacaaggcgaatggcaaccagctacattacagcgtttgagaaatgcatggaacgtttttgtgagatggtgtacgcaccaaggtattccggctctgcctgcacgtcatcaggatgttgaacgttatctgatcgaacgccgtaatgaattacaccgcaacaccctgaaagttcatttatgggccattggcaagactcacgtgatcagtggcctgcctaatccatgcgcgcatcgttacgtcaaagcccaaatggcgcagattactcatcaaaaagtacgcgaacgtgaaagaatcgaacaggctcctgcattccgcgaatccgatctggaccgtttaaccgaattgtggtccgctacaagatcggtcacgcaacagcgcgatttgatgattgtttctctggcttatgaaactctgttgcgtaagaacaacctggaacaaatgaaagtgggtgatattgaattttgtcaggacggcagcgccctgattacaatccctttctctaaaacgaaccactcaggtcgcgatgacgtccgttggattagtccacaagtcgctaatcaggtacatgcatacctgcaattacctaacatcgatgccgacccacaatgctttttgctgcaacgtgttaaacgttccggcaaggcgttgaatccagaatcgcataataccctgaacggccatcacccggttagcgaaaaattaattagtagagtgttcgaaagagcttggcgcgcattgaaccacgaaactggtccacgttatactggccattctgcaagagtgggtgctgcacaagatttattgcaggaaggctactcaacactgcaagttatgcaggctggtggctggtcttcagaaaaaatggtgttacgttatggcagacatttgcacgcacatacgtctgctatggctcaaaagagacgccagcgctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z