BBa_K1641005 1 BBa_K1641005 Fusion protein of Flp-EGFP-ssra, with RBS at beginning 2015-09-08T11:00:00Z 2015-09-10T03:02:39Z Flp is from BBa_K1015001 and EGFP::ssra-tag from pBI121-EGFP in Yongjun Lu's lab is fused by overlap-PCR; others through connection of bricks. This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp::EGFP::ssra-tag. This part is for expression of Flp::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer. Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need. false false _1708_ 20036 20036 9 false Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. C-term EGFP fusion on Flp seems have far better activity than N-term fusion. false Pai Li annotation2447943 1 Flp (without stop) range2447943 1 19 1286 annotation2447940 1 B0034 range2447940 1 1 12 annotation2447941 1 scar range2447941 1 13 18 annotation2447942 1 flexible chain range2447942 1 1287 1311 annotation2447944 1 EGFP-ssra(M0051) range2447944 1 1312 2070 BBa_K1641005_sequence 1 aaagaggagaaaactagaatgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcataggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z