BBa_K1641006 1 BBa_K1641006 Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning 2015-09-08T11:00:00Z 2015-09-13T10:53:02Z Vcre from BBa_1641000 (de novo synthesized gene) and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks. This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Vcre::EGFP::ssra-tag. The part is for expression of Vcre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer. Vcre (BBa_1641000) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. A EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression. false false _2058_ 24860 20036 9 false Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Vcre is according to its structure is similar to Cre, and hence the C-term EGFP fusion tend not to interfere the activity. false Pai Li annotation2447956 1 EGFP::ssra range2447956 1 1183 1941 annotation2447951 1 B0034 range2447951 1 1 12 annotation2447953 1 Flexible chain range2447953 1 1159 1182 annotation2447952 1 Vcre range2447952 1 19 1158 BBa_K1641006_sequence 1 aaagaggagaaaactagaatgattgaaaatcaactgtccctgctgggtgatttttcgggcgttcgtcctgatgacgtgaaaacagccattcaagctgcacagaaaaagggtatcaatgttgcggaaaacgaacagtttaaagccgcgttcgaacatttgctgaacgaatttaaaaagagagaagaacgctactccccaaacaccttgcgtagactggaatcggcctggacttgtttcgttgactggtgcttagccaatcatagacacagcttgcctgcgaccccagatactgtggaagcatttttcattgaacgcgcagaagaactgcatcgtaacacattaagcgtctatagatgggctatcagtcgtgttcacagagtggcaggttgtccagatccgtgcctggacatttacgtggaagatcgcttaaaagccatcgcgagaaaaaaggtccgcgaaggcgaagccgtaaagcaagcgagtccatttaacgaacagcatttattgaaactgacgtccttgtggtatcgctcggataagctgttattgcgccgtaatctggcgctgttagctgtcgcatacgaatctatgttgagagcttcagaactggcaaacattcgcgtatctgatatggaattggccggtgacggcacagcgatcctgacgattccgatcacaaaaacgaatcactcaggtgaacctgatacatgtattctgagccaagacgttgtgagtttgctgatggattatacggaagctggcaaactggatatgtcttcagacggttttctgttcgtcggcgtttctaagcataacacatgcatcaagccaaaaaaggataagcaaacgggtgaagttctgcacaaaccgatcaccactaagaccgtcgaaggcgtattttacagtgcatgggaaactctggacttaggtcgtcagggcgttaaaccgttcaccgctcattccgcaagagtgggtgctgcacaagatttattgaaaaagggctacaacactctgcaaattcaacagtcaggtcgctggagcagtggcgctatggttgcgcgctacggtcgtgctatcttagcacgtgatggcgctatggctcactctcgtgttaaaactagatcagctcctatgcagtggggtaaagatgaaaaggacggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z