BBa_K1641007 1 BBa_K1641007 Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning 2015-09-08T11:00:00Z 2015-09-13T10:52:29Z Scre from BBa_K1641001 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks. This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Scre::EGFP::ssra-tag. The part is for expression of Scre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer. Scre (BBa_K1641001) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel SloxP (or its derivatives) sites up-side-down. An EGFP fusion at the C-term of Scre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression. false false _2058_ 24860 20036 9 false Flexible chain works to keep two parts away to avoid misfolding; ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Scre has similar structure of Cre, and hence have far better performance if EGFP is fused to its C-term rather than N-term. false Pai Li BBa_K1641007_sequence 1 atgtccctgttaaccactaataaccattcagtggctctgagctatggtgaaccaccgagcaccttgaatgatagtctgaaagactcctaccaaagatcgactgatgaactgcaagctctgctgtctaaaccgttagcccaattgacagatgcggacaagttacgtattcgtgaaatcacgcaggcaaaactgaagcatttcttggacaatggccaccgcacacgtagagctaacacgtggcgcgcattaatgtctcgttgggccaaatttgaatcatggtgtttgaccaataacctgactcctttaccagctacccctgaagttgtggcaactttcattgaatattaccaagcgtcttcatatacaacgctgagccagtacgcttgggcaatcaatagttttcatgttgaatgcggtttattgtcacctgtcagcagtaaaacagtacaagataagcagaacgaaattcgtatcgtgaaattggaatctggtggcctggctcaagaacaggcaactccattcagattgcatcacctgcaaatgttaattgaatcttatggcgaatcagaaagactgttagataaacgcaatctggccttgctgaacattgcgtacgaatccttattgcgtgaatcggaactgttaagaatcaaagtcggtcacttgaagtccacctttgaaggcgattatgtcctgtctgttccatacaccaaaactaatgactccggtgaagaagaagtcgtaaacattacaccgctgggctttaaactgattcaacgctatatccagggtgctggcttaacgaaggaagattacttgttccaaccgatcggtcgtagcaataaagttagtgtgcaggccaagcctatgagcacacgtacggttgatagagtgtttctgtgggcgttcgaaagtttaggcattgacagacattccgcatggtcgggtcactctgcacgtatcggtgctgcacaggatttgctggccgcgggttattctattgcccaaatccaggaaaacggccgctggaaatcaccaatgatggttctgcgttatggcaaggacattaaagctaaggaatcagctatggctaaaatgctggcagaacgccgtggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z