BBa_K1641014
1
BBa_K1641014
Up-side-down RBS::mcherry-ssra with loxP recognition sites.
2015-09-09T11:00:00Z
2015-09-10T07:33:10Z
RBS and loxP are added to mcherry by cut-and-link or PCR.
This is a special RBS::mcherry-ssra inverted into up-side-down form, with two LoxP sites at the upstream and downstream of the gene. LoxP can be recognized and the middle sequence can be restored by invertase Cre. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.
This part can be used to construct the reporter for real-time invertase dynamics analysis.
false
false
_2058_
20036
20036
9
false
This sequence is up-side-down, hence can be inverted into normal sequence.
false
Pai Li
annotation2448567
1
LoxP
range2448567
1
812
845
annotation2448565
1
Inverted mcherry-ssra
range2448565
1
44
787
annotation2448566
1
BBa_B0034
range2448566
1
794
805
annotation2448564
1
LoxP
range2448564
1
1
34
BBa_K1641014_sequence
1
ataacttcgtatagcatacattatacgaagttatactagattattaagaagcgtcagcgtagttttcgtcgttagcagccttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccgggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgtcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgccgtcctgcaaggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatgtcggcggggtgcttcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccttgctcaccattctagttttctcctcttttctagtataacttcgtataatgtatgctatacgaagttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z