BBa_K1641016 1 BBa_K1641016 Up-side-down RBS::mcherry-ssra with Rox, recognition site of Dre 2015-09-09T11:00:00Z 2015-09-10T11:17:59Z Constructed by PCR or cut-and-link of previous bricks. This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Rox sites at the upstream and downstream of the gene. Rox can be recognized and the middle sequence can be restored by invertase Dre (BBa_K1641002). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression. This part can be used to construct the reporter for real-time invertase dynamics analysis. false false _2058_ 20036 20036 9 false This sequence is up-side-down, hence can be inverted into normal sequence. false Pai Li annotation2448616 1 Rox range2448616 1 1 32 annotation2448618 1 B0034 range2448618 1 792 803 annotation2448617 1 inverted mcherry-ssra range2448617 1 42 785 annotation2448619 1 Rox range2448619 1 810 841 BBa_K1641016_sequence 1 taactttaaataattggcattatttaaagttaactagattattaagaagcgtcagcgtagttttcgtcgttagcagccttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccgggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgtcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgccgtcctgcaaggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatgtcggcggggtgcttcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccttgctcaccattctagttttctcctcttttctagttaactttaaataatgccaattatttaaagtta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z