BBa_K1641017
1
BBa_K1641017
Up-side-down RBS::mcherry-ssra with Vox, Vika recognition sites
2015-09-10T11:00:00Z
2015-09-11T09:50:19Z
Constructed by PCR or cut-and-link of previous bricks.
This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Vox sites at the upstream and downstream of the gene. Vox can be recognized and the middle sequence can be restored by invertase Vika (BBa_K1641003). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.
This part can be used to construct the reporter for real-time invertase dynamics analysis.
false
false
_2058_
20036
20036
9
false
This sequence is up-side-down, hence can be inverted into normal sequence.
false
Pai Li
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z