BBa_K1641018 1 BBa_K1641018 Up-side-down RBS::mcherry-ssra with SloxM1, Scre recognition sites 2015-09-09T11:00:00Z 2015-09-10T11:58:35Z This part is constructed by PCR or cut-and-link of biobricks. This is a special RBS::mcherry-ssra inverted into up-side-down form, with two SloxM1 sites at the upstream and downstream of the gene. SloxM1 can be recognized by invertase Scre and the middle mcherry can be restored through inversion. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression. This part can be used to construct the reporter for real-time invertase dynamics analysis. false false _2058_ 20036 20036 9 false This sequence is up-side-down, hence can be inverted into normal sequence. false Pai Li annotation2448621 1 inverted mcherry-ssra range2448621 1 44 787 annotation2448622 1 B0034 range2448622 1 794 805 annotation2448623 1 SloxM1 range2448623 1 812 845 annotation2448620 1 SloxM1 range2448620 1 1 34 BBa_K1641018_sequence 1 ctcgtgtccgataactgtaattatcggacacgagactagattattaagaagcgtcagcgtagttttcgtcgttagcagccttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccgggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgtcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgccgtcctgcaaggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatgtcggcggggtgcttcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccttgctcaccattctagttttctcctcttttctagtctcgtgtccgataattacagttatcggacacgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z