BBa_K1641024
1
BBa_K1641024
Reporter of Invertase activity of Cre, pInv-rep-100LoxM
2015-09-09T11:00:00Z
2015-09-12T03:51:10Z
All of the parts come from biobricks.
This is the reporter of inversion activity of invertase Cre. An up-side-down mcherry gene with RBS is surrounded by a pair of LoxP and controlled by a constructive promoter BBa_J23101. At first stage, due to the meaningless mRNA, red signal cannot be generated, but when Cre exist,it inverts and restore the mcherry gene between two LoxP, the red signal can be generated at once.
This device is constructed to measure the real-time dynamics of timing module of Cre in the Micro-timer presented by SYSU-CHINA.
false
false
_2058_
20036
20036
9
In stock
false
We aim to construct a real-timer detector of invertase dynamics, which must be quantifiable. Hence, using mcherry, one of the best fluorescence signal, we can supervise the RFU of a strain and precisely simulate the exact situation of each invertase timing module. The data can be valuable for modeling work and the inversion time of corresponding module can be calculated.
false
Pai Li
component2448535
1
BBa_J23101
annotation2448535
1
BBa_J23101
range2448535
1
1
35
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1641024_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z