BBa_K1641039
1
BBa_K1641039
Inductive invertase generating vector - pInv-gen-Scre-EGFP-ssra
2015-09-11T11:00:00Z
2015-09-12T11:33:35Z
All invertase generators are constructed as following structure:
AraC+Pbad::RBS::invertase-EGFP(-ssra)::terminator
This is a complete invertase-EGFP expressing device induced by arabinose
false
false
_2058_
20036
20036
9
false
This brick is constructed through cut-and-link of other bricks without risky sources, usually E. coli originated genes.
false
Pai Li
component2452790
1
BBa_B1006
component2452780
1
BBa_I0500
annotation2452790
1
BBa_B1006
range2452790
1
3171
3209
annotation2452780
1
BBa_I0500
range2452780
1
1
1210
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898428
1
B1006
range1898428
1
1
39
annotation1898431
1
PolyA
range1898431
1
1
9
BBa_I0500
1
pBad/araC
Inducible pBad/araC promoter
2003-12-05T12:00:00Z
2015-08-31T04:07:29Z
-- No description --
false
true
_1_
0
24
7
In stock
false
true
Sri Kosuri
annotation1498842
1
PC
range1498842
1
1043
1114
annotation1498841
1
Pbad
range1498841
1
1080
1210
annotation1498843
1
AraC
range1498843
1
1
1079
BBa_K1641039_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaaactagaatgattgaaaatcaactgtccctgctgggtgatttttcgggcgttcgtcctgatgacgtgaaaacagccattcaagctgcacagaaaaagggtatcaatgttgcggaaaacgaacagtttaaagccgcgttcgaacatttgctgaacgaatttaaaaagagagaagaacgctactccccaaacaccttgcgtagactggaatcggcctggacttgtttcgttgactggtgcttagccaatcatagacacagcttgcctgcgaccccagatactgtggaagcatttttcattgaacgcgcagaagaactgcatcgtaacacattaagcgtctatagatgggctatcagtcgtgttcacagagtggcaggttgtccagatccgtgcctggacatttacgtggaagatcgcttaaaagccatcgcgagaaaaaaggtccgcgaaggcgaagccgtaaagcaagcgagtccatttaacgaacagcatttattgaaactgacgtccttgtggtatcgctcggataagctgttattgcgccgtaatctggcgctgttagctgtcgcatacgaatctatgttgagagcttcagaactggcaaacattcgcgtatctgatatggaattggccggtgacggcacagcgatcctgacgattccgatcacaaaaacgaatcactcaggtgaacctgatacatgtattctgagccaagacgttgtgagtttgctgatggattatacggaagctggcaaactggatatgtcttcagacggttttctgttcgtcggcgtttctaagcataacacatgcatcaagccaaaaaaggataagcaaacgggtgaagttctgcacaaaccgatcaccactaagaccgtcgaaggcgtattttacagtgcatgggaaactctggacttaggtcgtcagggcgttaaaccgttcaccgctcattccgcaagagtgggtgctgcacaagatttattgaaaaagggctacaacactctgcaaattcaacagtcaggtcgctggagcagtggcgctatggttgcgcgctacggtcgtgctatcttagcacgtgatggcgctatggctcactctcgtgttaaaactagatcagctcctatgcagtggggtaaagatgaaaaggacggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_I0500_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z