LOCUS       BBa_K1641040            3095 bp    DNA     linear       26-Nov-2024
DEFINITION  Inductive invertase generating vector - pInv-gen-Dre-EGFP-ssra
ACCESSION   BBa_K1641040
VERSION     BBa_K1641040.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1230
                     /label=B0034
     CDS             1237..2262
                     /label=Dre (in fusion)
     misc_feature    2263..2286
                     /label=Flexible chain
     CDS             2287..3045
                     /label=EGFP-ssra (in fusion)
     misc_feature    1219..3048
                     /label=BBa_K1641008
     misc_feature    3057..3095
                     /label=B1006
     stem_loop       3066..3087
                     /label=modified thr terminator
     polyA_site      3088..3095
                     /label=PolyA
     polyA_site      3057..3065
                     /label=PolyA
     terminator      3057..3095
                     /label=BBa_B1006
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa actagaatga gcgaactgat tatcagtggt
     1261 tcttcaggtg gctttttacg caacatcggc aaggaatacc aagaagctgc tgaaaacttc
     1321 atgcgtttca tgaacgatca gggtgcttac gcaccaaaca ctttacgcga cttgcgtctg
     1381 gtgttccatt cttgggctcg ttggtgtcac gccagacaac tggcgtggtt tccgatttct
     1441 cctgaaatgg cacgtgaata tttcttacag ttgcatgatg ctgacttggc atctaccact
     1501 atcgataaac attacgctat gctgaatatg ctgttatcac actgcggcct gccaccgtta
     1561 agcgatgaca agagcgtcag tctggccatg cgtcgtattc gtcgtgaagc cgcgacggaa
     1621 aaaggtgaaa gaaccggcca agcgatcccg ttacgctggg atgacttgaa gttgctggat
     1681 gtcttattgt ccagatcgga acgcttggta gacctgcgca atcgtgcttt tctgttcgtt
     1741 gcatataaca cgttaatgcg tatgtctgaa atttcaagaa tccgcgttgg tgatttagac
     1801 cagacaggcg atactgtgac attgcatatt tctcacacga aaaccatcac aaccgctgct
     1861 ggtctggata aggttttgag tagacgcacc actgctgttc tgaatgattg gttagacgtg
     1921 tccggcttga gagaacatcc tgatgccgtg ctgtttcctc caattcaccg ctcaaacaaa
     1981 gcgcgtatca caacgacccc actgactgcc ccggcgatgg aaaagatttt cagcgatgcc
     2041 tgggtcctgt taaataaaag agacgcgacc cctaacaagg gtcgttatag aacttggaca
     2101 ggtcattcgg ctcgcgtagg cgccgcgatt gatatggcag aaaaacaagt cagtatggta
     2161 gaaatcatgc aggaaggtac ttggaaaaag ccagaaacat tgatgcgtta cctgcgtaga
     2221 ggtggcgttt ccgtgggcgc taattctcgt ctgatggatt ctggagctga tacaggtagc
     2281 ggaggtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2341 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2401 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2461 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2521 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2701 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2761 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2881 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2941 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3001 aaggctgcta acgacgaaaa ctacaactac gctgacgctt cttaataata ctagagaaaa
     3061 aaaaaccccg cccctgacag ggcggggttt ttttt
//