BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898428 1 B1006 range1898428 1 1 39 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K1641040 1 BBa_K1641040 Inductive invertase generating vector - pInv-gen-Dre-EGFP-ssra 2015-09-11T11:00:00Z 2015-09-12T11:35:32Z All invertase generators are constructed as following structure: AraC+Pbad::RBS::invertase-EGFP(-ssra)::terminator This is a complete invertase-EGFP expressing device induced by arabinose false false _2058_ 20036 20036 9 false This brick is constructed through cut-and-link of other bricks without risky sources, usually E. coli originated genes. false Pai Li component2452799 1 BBa_K1641008 component2452794 1 BBa_I0500 component2452804 1 BBa_B1006 annotation2452799 1 BBa_K1641008 range2452799 1 1219 3048 annotation2452804 1 BBa_B1006 range2452804 1 3057 3095 annotation2452794 1 BBa_I0500 range2452794 1 1 1210 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498842 1 PC range1498842 1 1043 1114 BBa_K1641008 1 BBa_K1641008 Fusion protein of Dre::EGFP::ssra-tag, with RBS at beginning 2015-09-09T11:00:00Z 2015-09-10T12:01:46Z Dre from BBa_K1641002 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks. This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Dre::EGFP::ssra-tag. The part is for expression of Dre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer. Dre (BBa_K1641002) in our bricks is a Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. An EGFP fusion at the C-term of Dre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression. Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. This deletion do not interfere the usage of the brick. false false _2058_ 20036 20036 9 false Ssra-Tag: for efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Dre is a Cre-like recombinase, so C-term EGFP fusion will not significant reduce its activity. false Pai Li annotation2448209 1 Flexible chain range2448209 1 1045 1068 annotation2448207 1 B0034 range2448207 1 1 12 annotation2448208 1 Dre (in fusion) range2448208 1 19 1044 annotation2448212 1 EGFP-ssra (in fusion) range2448212 1 1069 1827 BBa_K1641008_sequence 1 aaagaggagaaaactagaatgagcgaactgattatcagtggttcttcaggtggctttttacgcaacatcggcaaggaataccaagaagctgctgaaaacttcatgcgtttcatgaacgatcagggtgcttacgcaccaaacactttacgcgacttgcgtctggtgttccattcttgggctcgttggtgtcacgccagacaactggcgtggtttccgatttctcctgaaatggcacgtgaatatttcttacagttgcatgatgctgacttggcatctaccactatcgataaacattacgctatgctgaatatgctgttatcacactgcggcctgccaccgttaagcgatgacaagagcgtcagtctggccatgcgtcgtattcgtcgtgaagccgcgacggaaaaaggtgaaagaaccggccaagcgatcccgttacgctgggatgacttgaagttgctggatgtcttattgtccagatcggaacgcttggtagacctgcgcaatcgtgcttttctgttcgttgcatataacacgttaatgcgtatgtctgaaatttcaagaatccgcgttggtgatttagaccagacaggcgatactgtgacattgcatatttctcacacgaaaaccatcacaaccgctgctggtctggataaggttttgagtagacgcaccactgctgttctgaatgattggttagacgtgtccggcttgagagaacatcctgatgccgtgctgtttcctccaattcaccgctcaaacaaagcgcgtatcacaacgaccccactgactgccccggcgatggaaaagattttcagcgatgcctgggtcctgttaaataaaagagacgcgacccctaacaagggtcgttatagaacttggacaggtcattcggctcgcgtaggcgccgcgattgatatggcagaaaaacaagtcagtatggtagaaatcatgcaggaaggtacttggaaaaagccagaaacattgatgcgttacctgcgtagaggtggcgtttccgtgggcgctaattctcgtctgatggattctggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaataa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1641040_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaaactagaatgagcgaactgattatcagtggttcttcaggtggctttttacgcaacatcggcaaggaataccaagaagctgctgaaaacttcatgcgtttcatgaacgatcagggtgcttacgcaccaaacactttacgcgacttgcgtctggtgttccattcttgggctcgttggtgtcacgccagacaactggcgtggtttccgatttctcctgaaatggcacgtgaatatttcttacagttgcatgatgctgacttggcatctaccactatcgataaacattacgctatgctgaatatgctgttatcacactgcggcctgccaccgttaagcgatgacaagagcgtcagtctggccatgcgtcgtattcgtcgtgaagccgcgacggaaaaaggtgaaagaaccggccaagcgatcccgttacgctgggatgacttgaagttgctggatgtcttattgtccagatcggaacgcttggtagacctgcgcaatcgtgcttttctgttcgttgcatataacacgttaatgcgtatgtctgaaatttcaagaatccgcgttggtgatttagaccagacaggcgatactgtgacattgcatatttctcacacgaaaaccatcacaaccgctgctggtctggataaggttttgagtagacgcaccactgctgttctgaatgattggttagacgtgtccggcttgagagaacatcctgatgccgtgctgtttcctccaattcaccgctcaaacaaagcgcgtatcacaacgaccccactgactgccccggcgatggaaaagattttcagcgatgcctgggtcctgttaaataaaagagacgcgacccctaacaagggtcgttatagaacttggacaggtcattcggctcgcgtaggcgccgcgattgatatggcagaaaaacaagtcagtatggtagaaatcatgcaggaaggtacttggaaaaagccagaaacattgatgcgttacctgcgtagaggtggcgtttccgtgggcgctaattctcgtctgatggattctggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggctgctaacgacgaaaactacaactacgctgacgcttcttaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z