BBa_K1641042
1
BBa_K1641042
Fusion protein of Cre-EGFP, with RBS at the beginning
2015-09-09T11:00:00Z
2015-09-10T07:14:11Z
Cre from BBa_K1179058 and EGFP from pBI121-EGFP is fused by overlap-PCR; RBS is added via PCR primer.
This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Cre-EGFP. The part is for expression of Cre-EGFP, which aims to overturn our invertase modules in our Micro-timer.
Cre (BBa_K1179058) is a most commonly used recombinase with invertase activity that turns the sequence between two anti-parallel LoxP sites up-side-down. An EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. Unlike other invertase module in Micro-timer of SYSU-CHINA in 2015, this part does not have ssra-tag, and thus has better expression efficiency and enzymatic activity.
Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.
false
false
_2058_
20036
20036
9
false
This Cre-EGFP do not process ssra, and hence much stable in vivo. As a result, the enzymatic activity seems much higher than those with ssra. Yet, a tiny leakage express might happen even if linked to inductive promoter.
false
Pai Li
annotation2448513
1
Cre (in fusion)
range2448513
1
19
960
annotation2448514
1
flexible chain
range2448514
1
961
984
annotation2448512
1
B0034
range2448512
1
1
12
annotation2448515
1
EGFP (in fusion)
range2448515
1
985
1704
BBa_K1641042_sequence
1
aaagaggagaaaactagaatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggttttccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatggagctgatacaggtagcggaggtatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z