LOCUS       BBa_K1641058            1016 bp    DNA     linear       22-Dec-2024
DEFINITION  pBAD-B0034-Killerred-B0015
ACCESSION   BBa_K1641058
VERSION     BBa_K1641058.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    874..879
                     /label=Double Stop
     misc_feature    157..159
                     /label=Start
     CDS             157..873
                     /label=cds
     misc_feature    364..366
                     /label=Phototoxic Mutation Glu(70)
     misc_feature    523..525
                     /label=Phototoxic Mutation Ser(121)
     misc_feature    355..363
                     /label=Chromophore
     misc_feature    595..597
                     /label=Phototoxic Mutation Asn(147)
     misc_feature    643..645
                     /label=Phototoxic Mutation Ala(163)
     CDS             157..879
                     /label=BBa_K1184000
     stem_loop       899..942
                     /label=stem_loop
     misc_feature    888..967
                     /label=BBa_B0010
     terminator      888..967
                     /label=BBa_B0010
     stem_loop       983..1002
                     /label=T7 TE
     misc_feature    1009..1009
                     /label=stop
     polyA_site      1003..1016
                     /label=polya
     misc_feature    976..1016
                     /label=BBa_B0012
     terminator      976..1016
                     /label=BBa_B0012
     terminator      888..1016
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg gttcagaggg cggccccgcc
      181 ctgttccaga gcgacatgac cttcaaaatc ttcatcgacg gcgaggtgaa cggccagaag
      241 ttcaccatcg tggccgacgg cagcagcaag ttcccccacg gcgacttcaa cgtgcacgcc
      301 gtgtgcgaga ccggcaagct gcccatgagc tggaagccca tctgccacct gatccagtac
      361 ggcgagccct tcttcgcccg ctaccccgac ggcatcagcc atttcgccca ggagtgcttc
      421 cccgagggcc tgagcatcga ccgcaccgtg cgcttcgaga acgacggcac catgaccagc
      481 caccacacct acgagctgga cgacacctgc gtggtgagcc gcatcaccgt gaactgcgac
      541 ggcttccagc ccgacggccc catcatgcgc gaccagctgg tggacatcct gcccaacgag
      601 acccacatgt tcccccacgg ccccaacgcc gtgcgccagc tggccttcat cggcttcacc
      661 accgccgacg gcggcctgat gatgggccac ttcgacagca agatgacctt caacggcagc
      721 cgcgccatcg agatccccgg cccacacttc gtgaccatca tcaccaagca gatgagggac
      781 accagcgaca agcgcgacca cgtgtgccag cgcgaggtgg cctacgccca cagcgtgccc
      841 cgcatcacca gcgccatcgg tagcgacgag gattaataat actagagcca ggcatcaaat
      901 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      961 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//