BBa_K1641219
1
BBa_K1641219
Standard for qPCR
2015-09-12T11:00:00Z
2015-09-14T09:04:53Z
Artificial.
Reversed BBa_J61046.
false
false
_2058_
23346
23346
9
false
For Cre recombinase.
false
Jianheng Liu
BBa_I13453
1
BBa_I13453
Pbad promoter
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
PBad promoter from I0500 without AraC.
false
false
_11_
0
253
6
In stock
false
true
jkm
BBa_J61020
1
FRT
[FRT]
2006-09-20T11:00:00Z
2015-08-31T02:02:59Z
<tt>
Extend ca1010F/R (73 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13522 (EcoRI/SpeI)<br>
Product is pSB1A2-Bca1010<br>
----
ca1010F Forward (universal biobrick) EcoRI for FRT<br>
GGACTgaattcgcggccgcttctagag<br>
ca1010R Reverse SpeI oligo for FRT<br>
cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg<br>
</tt>
Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
false
false
_95_
0
483
95
It's complicated
false
N/A
false
John Anderson
BBa_K1641200
1
BBa_K1641200
pBAD(forward)-FRT(forward)-LoxP(reverse)
2015-09-12T11:00:00Z
2015-09-13T06:27:34Z
Sequence was synthetized by IDT.
pBAD forward promoter+recombinase Flpe recognition site (forward)+recombinase Cre recognition site (reverse).
Used in iGEM15_SYSU_China project.
false
false
_2058_
23346
23346
9
false
This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment.
Two primers are:
GCACCTTGACTCTGACAATCCT (forward)
GCCTGTGCTAATGGTGATGACT (reverse)
they both have a high annealing temperature (about 58C). They can be used as qPCR primers.
The restriction enzyme sites are HindIII and BanI
false
Jianheng Liu
component2453177
1
BBa_J61020
component2453176
1
BBa_I13453
component2453178
1
BBa_K1641219
annotation2453178
1
BBa_K1641219
range2453178
1
181
214
annotation2453177
1
BBa_J61020
range2453177
1
139
172
annotation2453176
1
BBa_I13453
range2453176
1
1
130
BBa_I13453_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_K1641219_sequence
1
gcaccttgactctgacaatcctttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtcatcaccattagcacaggc
BBa_J61020_sequence
1
gaagttcctatactttttagagaataggaacttc
BBa_K1641200_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggaagttcctatactttttagagaataggaacttctactagagataacttcgtataatgtatgctatacgaagttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z