BBa_K1641206 1 BBa_K1641206 FRT(reverse)-loxP(forward)-pBAD(reverse) 2015-09-13T11:00:00Z 2015-09-14T08:48:35Z Synthesized by IDT as an intermediate in iGEM15_SYSU_CHINA project. false false _2058_ 23346 23346 9 false This sequence has two primer sites FRT and loxP for further testing in our experiment. Two primers are: GCACCTTGACTCTGACAATCCT (forward) GCCTGTGCTAATGGTGATGACT (reverse) they both have a high annealing temperature (about 58C). They can be used as qPCR primers. false Jianheng Liu BBa_K1641206_sequence 1 gaagttcctattctctaaaaagtataggaacttctactagagagtcatcaccattagcacaggcggggtaccccgcaccttgactctgacaatccttactagagataacttcgtatagcatacattatacgaagttattactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z