LOCUS       BBa_K1641209            2257 bp    DNA     linear       23-Dec-2024
DEFINITION  flpe-ECFP
ACCESSION   BBa_K1641209
VERSION     BBa_K1641209.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1332
                     /label=BBa_K1641222
     CDS             1..1332
                     /label=BBa_K1641211
     misc_feature    1345..1348
                     /label=conserved
     RBS             1341..1352
                     /label=BBa_B0034
     CDS             1359..2120
                     /label=CFP (LVA)
     misc_feature    2077..2114
                     /label=SsrA
     misc_feature    2115..2120
                     /label=2
     misc_feature    1427..1427
                     /label=A
     misc_feature    1359..2120
                     /label=BBa_E0022
     misc_feature    1359..2120
                     /label=BBa_E0022
     stem_loop       2140..2183
                     /label=stem_loop
     misc_feature    2129..2208
                     /label=BBa_B0010
     terminator      2129..2208
                     /label=BBa_B0010
     stem_loop       2224..2243
                     /label=T7 TE
     misc_feature    2250..2250
                     /label=stop
     polyA_site      2244..2257
                     /label=polya
     misc_feature    2217..2257
                     /label=BBa_B0012
     terminator      2217..2257
                     /label=BBa_B0012
     misc_feature    1341..2257
                     /label=BBa_E0422
ORIGIN
        1 aaagaggaga aatactagat gagccagttt ggcatattat gtaaaacacc acctaaggtc
       61 ctggttcgtc agtttgtgga aaggtttgaa agaccttcag gggaaaaaat agcatcatgt
      121 gctgctgaac taacctattt atgttggatg attactcata acggaacagc aatcaagaga
      181 gccacattca tgagctataa tactatcata agcaattcgc tgagtttcga tattgtcaac
      241 aaatcactcc agtttaaata caagacgcaa aaagcaacaa ttctggaagc ctcattaaag
      301 aaattaattc ctgcttggga atttacaatt attccttaca atggacaaaa acatcaatct
      361 gatatcactg atattgtaag tagtttgcaa ttacagttcg aatcatcgga agaagcagat
      421 aagggaaata gccacagtaa aaaaatgctt aaagcacttc taagtgaggg tgaaagcatc
      481 tgggagatca ctgagaaaat actaaattcg tttgagtata cctcgagatt tacaaaaaca
      541 aaaactttat accaattcct cttcctagct actttcatca attgtggaag attcagcgat
      601 attaagaacg ttgatccgaa atcatttaaa ttagtccaaa ataagtatct gggagtaata
      661 atccagtgtt tagtgacaga gacaaagaca agcgttagta ggcacatata cttctttagc
      721 gcaaggggta ggatcgatcc acttgtatat ttggatgaat ttttgagaaa ttctgaacca
      781 gtcctaaaac gagtaaatag gaccggcaat tcttcaagca acaaacagga ataccaatta
      841 ttaaaagata acttagtcag atcgtacaac aaggctttga agaaaaatgc gccttatcca
      901 atctttgcta taaagaatgg cccaaaatct cacattggaa gacatttgat gacctcattt
      961 ctgtcaatga agggcctaac ggagttgact aatgttgtgg gaaattggag cgataagcgt
     1021 gcttctgccg tggccaggac aacgtatact catcagataa cagcaatacc tgatcactac
     1081 ttcgcgctag tttctcggta ctatgcatat gatccaatat caaaggaaat gatagcattg
     1141 aaggatgaga ctaatccaat tgaggagtgg cagcatatag aacagctaaa gggtagtgct
     1201 gaaggaagca tacgataccc cgcatggaat gggataatat cacaggaggt actagactac
     1261 ctttcatcct acataaatag acgcatagct gctaacgacg aaaactacaa ctacgctgac
     1321 gcttcttaat aatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc
     1381 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt
     1441 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1501 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1561 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1621 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1681 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1741 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1801 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1861 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1921 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1981 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2041 ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact
     2101 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2161 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2221 actggctcac cttcgggtgg gcctttctgc gtttata
//