LOCUS       BBa_K1641210            2546 bp    DNA     linear       27-Dec-2024
DEFINITION  mCherry-loxP(reverse)-GFP
ACCESSION   BBa_K1641210
VERSION     BBa_K1641210.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..729
                     /label=mCherry
     misc_feature    370..370
                     /label=C->T (removing PstI site)
     misc_feature    19..732
                     /label=BBa_J06504
     misc_feature    1..732
                     /label=BBa_J06602
     misc_feature    741..774
                     /label=BBa_K1641226
     RBS             783..797
                     /label=RBS-1\Strong
     RBS             790..794
                     /label=RBS
     misc_feature    783..797
                     /label=BBa_B0030
     RBS             783..797
                     /label=BBa_B0030
     CDS             804..1523
                     /label=GFP protein
     CDS             804..1523
                     /label=BBa_E0040
     stem_loop       1543..1586
                     /label=stem_loop
     misc_feature    1532..1611
                     /label=BBa_B0010
     terminator      1532..1611
                     /label=BBa_B0010
     stem_loop       1627..1646
                     /label=T7 TE
     misc_feature    1653..1653
                     /label=stop
     polyA_site      1647..1660
                     /label=polya
     misc_feature    1620..1660
                     /label=BBa_B0012
     terminator      1620..1660
                     /label=BBa_B0012
     misc_feature    783..1660
                     /label=BBa_E0840
     misc_feature    741..1660
                     /label=BBa_K1641208
     RBS             1669..1683
                     /label=RBS-1\Strong
     RBS             1676..1680
                     /label=RBS
     misc_feature    1669..1683
                     /label=BBa_B0030
     RBS             1669..1683
                     /label=BBa_B0030
     CDS             1690..2409
                     /label=GFP protein
     CDS             1690..2409
                     /label=BBa_E0040
     stem_loop       2429..2472
                     /label=stem_loop
     misc_feature    2418..2497
                     /label=BBa_B0010
     terminator      2418..2497
                     /label=BBa_B0010
     stem_loop       2513..2532
                     /label=T7 TE
     misc_feature    2539..2539
                     /label=stop
     polyA_site      2533..2546
                     /label=polya
     misc_feature    2506..2546
                     /label=BBa_B0012
     terminator      2506..2546
                     /label=BBa_B0012
     misc_feature    1669..2546
                     /label=BBa_E0840
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtaat aatactagag ataacttcgt ataatgtatg ctatacgaag ttattactag
      781 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      841 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      901 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      961 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1021 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     1081 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1141 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1201 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     1261 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1321 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1381 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1441 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1501 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1561 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1621 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagat taaagaggag
     1681 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1741 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1801 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1861 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1921 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1981 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     2041 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     2101 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     2161 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     2221 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     2281 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     2341 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     2401 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2461 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2521 ttcgggtggg cctttctgcg tttata
//