##gff-version 3
##sequence-region BBa_K1641210 1 2546
BBa_K1641210	.	engineered_region	1	732	.	+	0	ID=BBa_J06602;Name=BBa_J06602
BBa_K1641210	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06602
BBa_K1641210	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1641210	.	engineered_region	19	732	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06602
BBa_K1641210	.	sequence_alteration	370	370	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1641210	.	CDS	19	729	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1641210	.	engineered_region	741	1660	.	+	0	ID=BBa_K1641208;Name=BBa_K1641208
BBa_K1641210	.	engineered_region	783	1660	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K1641208
BBa_K1641210	.	ribosome_entry_site	783	797	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1641210	.	ribosome_entry_site	790	794	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1641210	.	engineered_region	783	797	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1641210	.	ribosome_entry_site	783	797	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1641210	.	terminator	1620	1660	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1641210	.	stop_codon	1653	1653	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1641210	.	polyA_site	1647	1660	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1641210	.	stem_loop	1627	1646	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1641210	.	engineered_region	1620	1660	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1641210	.	CDS	804	1523	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1641210	.	CDS	804	1523	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1641210	.	terminator	1532	1611	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1641210	.	engineered_region	1532	1611	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1641210	.	stem_loop	1543	1586	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1641210	.	sequence_feature	741	774	.	+	0	ID=BBa_K1641226;Name=BBa_K1641226;Parent=BBa_K1641208
BBa_K1641210	.	engineered_region	1669	2546	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1641210	.	ribosome_entry_site	1669	1683	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1641210	.	ribosome_entry_site	1676	1680	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1641210	.	engineered_region	1669	1683	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1641210	.	ribosome_entry_site	1669	1683	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1641210	.	terminator	2506	2546	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1641210	.	stop_codon	2539	2539	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1641210	.	polyA_site	2533	2546	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1641210	.	stem_loop	2513	2532	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1641210	.	engineered_region	2506	2546	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1641210	.	CDS	1690	2409	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1641210	.	CDS	1690	2409	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1641210	.	terminator	2418	2497	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1641210	.	engineered_region	2418	2497	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1641210	.	stem_loop	2429	2472	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1641210
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaagtaataatactagagataacttcgtataatgtatgctatacgaagttattactagagattaaaga
ggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtt
aatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgca
ctactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccc
agatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaa
gatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggta
ttgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggc
agacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagac
cattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccc
tttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttg
aattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttac
ccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaa
tgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaa
gaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatag
aatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacac
aatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaa
gcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacct
gtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgct
gggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata