BBa_K1641220
1
BBa_K1641220
pBAD(forward)-FRT(forward)-loxP(reverse)
2015-09-13T11:00:00Z
2015-09-14T08:13:25Z
Synthesized
This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment.
Two primers are:
GCACCTTGACTCTGACAATCCT (forward)
GCCTGTGCTAATGGTGATGACT (reverse)
they both have a high annealing temperature (about 58C). They can be used as qPCR primers.
The restriction enzyme sites are HindIII and BanI.
false
false
_2058_
23346
23346
9
false
false
Jianheng Liu
BBa_K1641220_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggaagttcctatactttttagagaataggaacttctactagagagtcatcaccattagcacaggcccaagcttggggggtaccccgcaccttgactctgacaatccttactagagataacttcgtataatgtatgctatacgaagttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z