##gff-version 3
##sequence-region BBa_K1641225 1 4702
BBa_K1641225	.	engineered_region	2698	3882	.	+	0	ID=BBa_K1641214;Name=BBa_K1641214
BBa_K1641225	.	sequence_feature	2698	2869	.	+	0	ID=BBa_K1641205;Name=BBa_K1641205;Parent=BBa_K1641214
BBa_K1641225	.	engineered_region	2878	3882	.	+	0	ID=BBa_K1641212;Name=BBa_K1641212;Parent=BBa_K1641214
BBa_K1641225	.	CDS	2896	3882	.	+	0	ID=BBa_K1641223;Name=BBa_K1641223;Parent=BBa_K1641212
BBa_K1641225	.	ribosome_entry_site	2878	2889	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1641212
BBa_K1641225	.	conserved_region	2882	2885	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1641225	.	sequence_feature	1	286	.	+	0	ID=BBa_K1641220;Name=BBa_K1641220
BBa_K1641225	.	engineered_region	3891	4702	.	+	0	ID=BBa_K1641213;Name=BBa_K1641213
BBa_K1641225	.	ribosome_entry_site	3891	3902	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1641213
BBa_K1641225	.	conserved_region	3895	3898	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1641225	.	terminator	4664	4702	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K1641213
BBa_K1641225	.	polyA_site	4664	4672	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1641225	.	polyA_site	4695	4702	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1641225	.	engineered_region	4664	4702	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1641225	.	stem_loop	4673	4694	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1641225	.	CDS	3909	4655	.	+	0	ID=BBa_K1641224;Name=BBa_K1641224;Parent=BBa_K1641213
BBa_K1641225	.	promoter	295	424	.	+	0	ID=BBa_J44002;Name=BBa_J44002
BBa_K1641225	.	promoter	295	424	.	+	0	ID=annotation2002776;Name=promoter;Parent=BBa_J44002
BBa_K1641225	.	CDS	433	2689	.	+	0	ID=BBa_K1641209;Name=BBa_K1641209
BBa_K1641225	.	CDS	433	1764	.	+	0	ID=BBa_K1641211;Name=BBa_K1641211;Parent=BBa_K1641209
BBa_K1641225	.	ribosome_entry_site	433	444	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1641211
BBa_K1641225	.	conserved_region	437	440	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1641225	.	CDS	451	1764	.	+	0	ID=BBa_K1641222;Name=BBa_K1641222;Parent=BBa_K1641211
BBa_K1641225	.	engineered_region	1773	2689	.	+	0	ID=BBa_E0422;Name=BBa_E0422;Parent=BBa_K1641209
BBa_K1641225	.	terminator	2561	2640	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_K1641225	.	engineered_region	2561	2640	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1641225	.	stem_loop	2572	2615	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1641225	.	ribosome_entry_site	1773	1784	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_K1641225	.	conserved_region	1777	1780	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1641225	.	terminator	2649	2689	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_K1641225	.	polyA_site	2676	2689	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1641225	.	engineered_region	2649	2689	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1641225	.	stop_codon	2682	2682	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1641225	.	stem_loop	2656	2675	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1641225	.	engineered_region	1791	2552	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_K1641225	.	CDS	1791	2552	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K1641225	.	point_mutation	1859	1859	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K1641225	.	stop_codon	2547	2552	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K1641225	.	engineered_region	1791	2552	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K1641225	.	tag	2509	2546	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
>BBa_K1641225
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggaagttcctatactttttag
agaataggaacttctactagagagtcatcaccattagcacaggcccaagcttggggggtaccccgcaccttgactctga
caatccttactagagataacttcgtataatgtatgctatacgaagttattactagaggctagcccaaaaaaacggtatg
gagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagca
aagtgtgacgccgtgcaaataatcaatgttactagagaaagaggagaaatactagatgagccagtttggcatattatgt
aaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtg
ctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataa
tactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaaca
attctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaat
ctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacag
taaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgag
tatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattca
gcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagt
gacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttg
gatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaat
accaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctat
aaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgact
aatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatac
ctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatga
gactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatgg
aatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatagctgctaacgacgaaaact
acaactacgctgacgcttcttaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttc
accggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagg
gcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgt
gaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcc
atgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagt
tcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaa
gctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaag
atccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccg
tgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggt
cctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaa
aactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagaggaagttcctattctctaaaaagtataggaacttctactagaggctagcccaaaaaaacggtatggaga
aacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagt
gtgacgccgtgcaaataatcaatgttactagagaaagaggagaaatactagatgttcagggatcgccaggcgttttctg
agcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcc
cgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacat
ttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgc
ggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgacca
ggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacacc
ctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccata
ttggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcg
atggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcg
ccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgcta
aggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgc
tggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctg
gatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatgctgctaacgacgaaaactacaactacgctgacg
cttcttaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaag
gagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggcc
gcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtc
ccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttcccc
gagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcagg
acggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccat
gggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctg
aaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctaca
acgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccg
ccactccaccggcggcatggacgagctgtacaaggctgctaacgacgaaaactacgctgacgcttcttaataatactag
agaaaaaaaaaccccgcccctgacagggcggggtttttttt