BBa_K1641225
type: ComponentDefinition
persistent identity: BBa K1641225
display id: BBa_K1641225
version: 1
was generated by: iGEM to SBOL conversion
description: Invertible element
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T10:06:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1641209
component: BBa_J44002
component: BBa_K1641220
component: BBa_K1641213
component: BBa_K1641214
sequence annotation: BBa_K1641220
sequence annotation: BBa_J44002
sequence annotation: BBa_K1641209
sequence annotation: BBa_K1641214
sequence annotation: BBa_K1641213
sequence: https://synbiohub.org/public/igem/BBa_K1641225_sequence/1

BBa_K1641209
type: Component
persistent identity: component2463737
display id: component2463737
version: 1
top level: BBa_K1641225
definition: BBa_K1641209
access: public

BBa_J44002
type: Component
persistent identity: component2463718
display id: component2463718
version: 1
top level: BBa_K1641225
definition: BBa_J44002
access: public

BBa_K1641220
type: Component
persistent identity: component2463716
display id: component2463716
version: 1
top level: BBa_K1641225
definition: BBa_K1641220
access: public

BBa_K1641213
type: Component
persistent identity: component2463752
display id: component2463752
version: 1
top level: BBa_K1641225
definition: BBa_K1641213
access: public

BBa_K1641214
type: Component
persistent identity: component2463743
display id: component2463743
version: 1
top level: BBa_K1641225
definition: BBa_K1641214
access: public

BBa_K1641220
type: SequenceAnnotation
persistent identity: annotation2463716
display id: annotation2463716
version: 1
top level: BBa_K1641225
location: https://synbiohub.org/public/igem/BBa_K1641225/annotation2463716/range2463716/1
component: BBa_K1641220

https://synbiohub.org/public/igem/BBa_K1641225/annotation2463716/range2463716/1
type: Range
persistent identity: range2463716
display id: range2463716
version: 1
top level: BBa_K1641225
direction: direction/reverse
start: 1
end: 286
orientation: inline

BBa_J44002
type: SequenceAnnotation
persistent identity: annotation2463718
display id: annotation2463718
version: 1
top level: BBa_K1641225
location: https://synbiohub.org/public/igem/BBa_K1641225/annotation2463718/range2463718/1
component: BBa_J44002

https://synbiohub.org/public/igem/BBa_K1641225/annotation2463718/range2463718/1
type: Range
persistent identity: range2463718
display id: range2463718
version: 1
top level: BBa_K1641225
direction: direction/reverse
start: 295
end: 424
orientation: inline

BBa_K1641209
type: SequenceAnnotation
persistent identity: annotation2463737
display id: annotation2463737
version: 1
top level: BBa_K1641225
location: https://synbiohub.org/public/igem/BBa_K1641225/annotation2463737/range2463737/1
component: BBa_K1641209

https://synbiohub.org/public/igem/BBa_K1641225/annotation2463737/range2463737/1
type: Range
persistent identity: range2463737
display id: range2463737
version: 1
top level: BBa_K1641225
direction: direction/reverse
start: 433
end: 2689
orientation: inline

BBa_K1641214
type: SequenceAnnotation
persistent identity: annotation2463743
display id: annotation2463743
version: 1
top level: BBa_K1641225
location: https://synbiohub.org/public/igem/BBa_K1641225/annotation2463743/range2463743/1
component: BBa_K1641214

https://synbiohub.org/public/igem/BBa_K1641225/annotation2463743/range2463743/1
type: Range
persistent identity: range2463743
display id: range2463743
version: 1
top level: BBa_K1641225
direction: direction/reverse
start: 2698
end: 3882
orientation: inline

BBa_K1641213
type: SequenceAnnotation
persistent identity: annotation2463752
display id: annotation2463752
version: 1
top level: BBa_K1641225
location: https://synbiohub.org/public/igem/BBa_K1641225/annotation2463752/range2463752/1
component: BBa_K1641213

https://synbiohub.org/public/igem/BBa_K1641225/annotation2463752/range2463752/1
type: Range
persistent identity: range2463752
display id: range2463752
version: 1
top level: BBa_K1641225
direction: direction/reverse
start: 3891
end: 4702
orientation: inline

BBa_K1641220
type: ComponentDefinition
persistent identity: BBa K1641220
display id: BBa_K1641220
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD(forward)-FRT(forward)-loxP(reverse)
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T08:13:25Z
mutable provenance: Synthesized
owned by: james
owned by: myers
mutable description: This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment. Two primers are: GCACCTTGACTCTGACAATCCT (forward) GCCTGTGCTAATGGTGATGACT (reverse) they both have a high annealing temperature (about 58C). They can be used as qPCR primers. The restriction enzyme sites are HindIII and BanI.
discontinued: false
dominant: false
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1641220_sequence/1

BBa_K1641209
type: ComponentDefinition
persistent identity: BBa K1641209
display id: BBa_K1641209
version: 1
was generated by: iGEM to SBOL conversion
description: flpe-ECFP
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T07:32:14Z
owned by: james
owned by: myers
mutable description: Flpe recombinase with ECFP reporter
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu & Yingnan Niu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_E0422
component: BBa_K1641211
sequence annotation: BBa_K1641211
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_K1641209_sequence/1

BBa_E0422
type: Component
persistent identity: component2454679
display id: component2454679
version: 1
top level: BBa_K1641209
definition: BBa_E0422
access: public

BBa_K1641211
type: Component
persistent identity: component2454665
display id: component2454665
version: 1
top level: BBa_K1641209
definition: BBa_K1641211
access: public

BBa_K1641211
type: SequenceAnnotation
persistent identity: annotation2454665
display id: annotation2454665
version: 1
top level: BBa_K1641209
location: https://synbiohub.org/public/igem/BBa_K1641209/annotation2454665/range2454665/1
component: BBa_K1641211

https://synbiohub.org/public/igem/BBa_K1641209/annotation2454665/range2454665/1
type: Range
persistent identity: range2454665
display id: range2454665
version: 1
top level: BBa_K1641209
direction: direction/reverse
start: 1
end: 1332
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2454679
display id: annotation2454679
version: 1
top level: BBa_K1641209
location: https://synbiohub.org/public/igem/BBa_K1641209/annotation2454679/range2454679/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_K1641209/annotation2454679/range2454679/1
type: Range
persistent identity: range2454679
display id: range2454679
version: 1
top level: BBa_K1641209
direction: direction/reverse
start: 1341
end: 2257
orientation: inline

BBa_K1641213
type: ComponentDefinition
persistent identity: BBa K1641213
display id: BBa_K1641213
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-mCherry-B1006
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:05:52Z
owned by: james
owned by: myers
mutable description: A qPCR friendly short terminator BBa_B1006 was inserted after mCherry。
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 4206
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu & Yingnan Niu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B1006
component: BBa_K1641224
sequence annotation: BBa_B0034
sequence annotation: BBa_K1641224
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1641213_sequence/1

BBa_B0034
type: Component
persistent identity: component2454687
display id: component2454687
version: 1
top level: BBa_K1641213
definition: BBa_B0034
access: public

BBa_B1006
type: Component
persistent identity: component2454693
display id: component2454693
version: 1
top level: BBa_K1641213
definition: BBa_B1006
access: public

BBa_K1641224
type: Component
persistent identity: component2454688
display id: component2454688
version: 1
top level: BBa_K1641213
definition: BBa_K1641224
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2454687
display id: annotation2454687
version: 1
top level: BBa_K1641213
location: https://synbiohub.org/public/igem/BBa_K1641213/annotation2454687/range2454687/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1641213/annotation2454687/range2454687/1
type: Range
persistent identity: range2454687
display id: range2454687
version: 1
top level: BBa_K1641213
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1641224
type: SequenceAnnotation
persistent identity: annotation2454688
display id: annotation2454688
version: 1
top level: BBa_K1641213
location: https://synbiohub.org/public/igem/BBa_K1641213/annotation2454688/range2454688/1
component: BBa_K1641224

https://synbiohub.org/public/igem/BBa_K1641213/annotation2454688/range2454688/1
type: Range
persistent identity: range2454688
display id: range2454688
version: 1
top level: BBa_K1641213
direction: direction/reverse
start: 19
end: 765
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2454693
display id: annotation2454693
version: 1
top level: BBa_K1641213
location: https://synbiohub.org/public/igem/BBa_K1641213/annotation2454693/range2454693/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1641213/annotation2454693/range2454693/1
type: Range
persistent identity: range2454693
display id: range2454693
version: 1
top level: BBa_K1641213
direction: direction/reverse
start: 774
end: 812
orientation: inline

BBa_K1641214
type: ComponentDefinition
persistent identity: BBa K1641214
display id: BBa_K1641214
version: 1
was generated by: iGEM to SBOL conversion
description: FRT(reverse)-pBAD(reverse)-Cre
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T07:34:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu & Yingnan Niu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1641212
component: BBa_K1641205
sequence annotation: BBa_K1641205
sequence annotation: BBa_K1641212
sequence: https://synbiohub.org/public/igem/BBa_K1641214_sequence/1

BBa_K1641212
type: Component
persistent identity: component2454701
display id: component2454701
version: 1
top level: BBa_K1641214
definition: BBa_K1641212
access: public

BBa_K1641205
type: Component
persistent identity: component2454697
display id: component2454697
version: 1
top level: BBa_K1641214
definition: BBa_K1641205
access: public

BBa_K1641205
type: SequenceAnnotation
persistent identity: annotation2454697
display id: annotation2454697
version: 1
top level: BBa_K1641214
location: https://synbiohub.org/public/igem/BBa_K1641214/annotation2454697/range2454697/1
component: BBa_K1641205

https://synbiohub.org/public/igem/BBa_K1641214/annotation2454697/range2454697/1
type: Range
persistent identity: range2454697
display id: range2454697
version: 1
top level: BBa_K1641214
direction: direction/reverse
start: 1
end: 172
orientation: inline

BBa_K1641212
type: SequenceAnnotation
persistent identity: annotation2454701
display id: annotation2454701
version: 1
top level: BBa_K1641214
location: https://synbiohub.org/public/igem/BBa_K1641214/annotation2454701/range2454701/1
component: BBa_K1641212

https://synbiohub.org/public/igem/BBa_K1641214/annotation2454701/range2454701/1
type: Range
persistent identity: range2454701
display id: range2454701
version: 1
top level: BBa_K1641214
direction: direction/reverse
start: 181
end: 1185
orientation: inline

BBa_J44002
type: ComponentDefinition
persistent identity: BBa J44002
display id: BBa_J44002
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD reverse
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligonucleotides.
owned by: james
owned by: myers
mutable description: This is the pBAD promoter (BBa_I13453) in the opposite orientation. It can be used to drive transcription in the direction of suffix to prefix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: true
creator: Brad Ogden
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J44002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2002776
display id: annotation2002776
version: 1
top level: BBa_J44002
location: https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
type: Range
persistent identity: range2002776
display id: range2002776
version: 1
top level: BBa_J44002
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
definition: ECFP
access: public

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
top level: BBa_E0422
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
top level: BBa_E0422
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
top level: BBa_E0422
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
top level: BBa_E0422
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_K1641205
type: ComponentDefinition
persistent identity: BBa K1641205
display id: BBa_K1641205
version: 1
was generated by: iGEM to SBOL conversion
description: FRT(reverse)-pBAD(reverse)
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T08:53:18Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1641205_sequence/1

BBa_K1641211
type: ComponentDefinition
persistent identity: BBa K1641211
display id: BBa_K1641211
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-flpe-ssrA(M0051)
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T07:33:18Z
owned by: james
owned by: myers
mutable description: Flpe recombinase BBa_K313002 with strong RBS(BBa_B0034) and ssrA tag(BBa_M0051), which can be translated strongly and eliminated in a short time if the flpe is not expressed continuely.
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1641222
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1641222
sequence: https://synbiohub.org/public/igem/BBa_K1641211_sequence/1

BBa_K1641222
type: Component
persistent identity: component2454661
display id: component2454661
version: 1
top level: BBa_K1641211
definition: BBa_K1641222
access: public

BBa_B0034
type: Component
persistent identity: component2454660
display id: component2454660
version: 1
top level: BBa_K1641211
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2454660
display id: annotation2454660
version: 1
top level: BBa_K1641211
location: https://synbiohub.org/public/igem/BBa_K1641211/annotation2454660/range2454660/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1641211/annotation2454660/range2454660/1
type: Range
persistent identity: range2454660
display id: range2454660
version: 1
top level: BBa_K1641211
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1641222
type: SequenceAnnotation
persistent identity: annotation2454661
display id: annotation2454661
version: 1
top level: BBa_K1641211
location: https://synbiohub.org/public/igem/BBa_K1641211/annotation2454661/range2454661/1
component: BBa_K1641222

https://synbiohub.org/public/igem/BBa_K1641211/annotation2454661/range2454661/1
type: Range
persistent identity: range2454661
display id: range2454661
version: 1
top level: BBa_K1641211
direction: direction/reverse
start: 19
end: 1332
orientation: inline

BBa_K1641212
type: ComponentDefinition
persistent identity: BBa K1641212
display id: BBa_K1641212
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-Cre-ssrA
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:06:53Z
owned by: james
owned by: myers
mutable description: Coding for Cre recombinase with ssrA tag. It can express Cre in high level and can be fast degraded if promoter is turned off.
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 4206
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_B0034
component: BBa_K1641223
sequence annotation: BBa_B0034
sequence annotation: BBa_K1641223
sequence: https://synbiohub.org/public/igem/BBa_K1641212_sequence/1

BBa_B0034
type: Component
persistent identity: component2454681
display id: component2454681
version: 1
top level: BBa_K1641212
definition: BBa_B0034
access: public

BBa_K1641223
type: Component
persistent identity: component2454682
display id: component2454682
version: 1
top level: BBa_K1641212
definition: BBa_K1641223
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2454681
display id: annotation2454681
version: 1
top level: BBa_K1641212
location: https://synbiohub.org/public/igem/BBa_K1641212/annotation2454681/range2454681/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1641212/annotation2454681/range2454681/1
type: Range
persistent identity: range2454681
display id: range2454681
version: 1
top level: BBa_K1641212
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1641223
type: SequenceAnnotation
persistent identity: annotation2454682
display id: annotation2454682
version: 1
top level: BBa_K1641212
location: https://synbiohub.org/public/igem/BBa_K1641212/annotation2454682/range2454682/1
component: BBa_K1641223

https://synbiohub.org/public/igem/BBa_K1641212/annotation2454682/range2454682/1
type: Range
persistent identity: range2454682
display id: range2454682
version: 1
top level: BBa_K1641212
direction: direction/reverse
start: 19
end: 1005
orientation: inline

BBa_K1641224
type: ComponentDefinition
persistent identity: BBa K1641224
display id: BBa_K1641224
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-ssrA
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:06:32Z
owned by: james
owned by: myers
mutable description: mCherry (BBa_J06504) with ssrA tag（BBa_M0052）
discontinued: false
dominant: false
group u list: _2058_
m user id: 4206
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1641224_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_K1641222
type: ComponentDefinition
persistent identity: BBa K1641222
display id: BBa_K1641222
version: 1
was generated by: iGEM to SBOL conversion
description: flpe-ssrA
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T09:28:46Z
owned by: james
owned by: myers
mutable description: Flpe recombinase with ssrA tag(BBa_M0051). Stop codon TAATAA was added.
discontinued: false
dominant: false
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1641222_sequence/1

BBa_K1641223
type: ComponentDefinition
persistent identity: BBa K1641223
display id: BBa_K1641223
version: 1
was generated by: iGEM to SBOL conversion
description: Cre-ssrA
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T09:32:19Z
owned by: james
owned by: myers
mutable description: Cre recombinase wit ssrA tag（BBa_M0051）. Stop codons TAATAA were added.
discontinued: false
dominant: false
group u list: _2058_
m user id: 23346
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Jianheng Liu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1641223_sequence/1

https://synbiohub.org/public/igem/BBa_K1641225_sequence/1
type: Sequence
persistent identity: BBa K1641225 sequence
display id: BBa_K1641225_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggaagttcctatactttttagagaataggaacttctactagagagtcatcaccattagcacaggcccaagcttggggggtaccccgcaccttgactctgacaatccttactagagataacttcgtataatgtatgctatacgaagttattactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagaaagaggagaaatactagatgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatagctgctaacgacgaaaactacaactacgctgacgcttcttaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641220_sequence/1
type: Sequence
persistent identity: BBa K1641220 sequence
display id: BBa_K1641220_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggaagttcctatactttttagagaataggaacttctactagagagtcatcaccattagcacaggcccaagcttggggggtaccccgcaccttgactctgacaatccttactagagataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641209_sequence/1
type: Sequence
persistent identity: BBa K1641209 sequence
display id: BBa_K1641209_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatagctgctaacgacgaaaactacaactacgctgacgcttcttaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641213_sequence/1
type: Sequence
persistent identity: BBa K1641213 sequence
display id: BBa_K1641213_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggctgctaacgacgaaaactacgctgacgcttcttaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641214_sequence/1
type: Sequence
persistent identity: BBa K1641214 sequence
display id: BBa_K1641214_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctattctctaaaaagtataggaacttctactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagaaagaggagaaatactagatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatgctgctaacgacgaaaactacaactacgctgacgcttcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44002_sequence/1
type: Sequence
persistent identity: BBa J44002 sequence
display id: BBa_J44002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0422_sequence/1
type: Sequence
persistent identity: BBa E0422 sequence
display id: BBa_E0422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641205_sequence/1
type: Sequence
persistent identity: BBa K1641205 sequence
display id: BBa_K1641205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctattctctaaaaagtataggaacttctactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641211_sequence/1
type: Sequence
persistent identity: BBa K1641211 sequence
display id: BBa_K1641211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatagctgctaacgacgaaaactacaactacgctgacgcttcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641212_sequence/1
type: Sequence
persistent identity: BBa K1641212 sequence
display id: BBa_K1641212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatgctgctaacgacgaaaactacaactacgctgacgcttcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641224_sequence/1
type: Sequence
persistent identity: BBa K1641224 sequence
display id: BBa_K1641224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggctgctaacgacgaaaactacgctgacgcttcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641222_sequence/1
type: Sequence
persistent identity: BBa K1641222 sequence
display id: BBa_K1641222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccagtttggcatattatgtaaaacaccacctaaggtcctggttcgtcagtttgtggaaaggtttgaaagaccttcaggggaaaaaatagcatcatgtgctgctgaactaacctatttatgttggatgattactcataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaacaaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattaattcctgcttgggaatttacaattattccttacaatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacctcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgagaaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaacaaacaggaataccaattattaaaagataacttagtcagatcgtacaacaaggctttgaagaaaaatgcgccttatccaatctttgctataaagaatggcccaaaatctcacattggaagacatttgatgacctcatttctgtcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcgctagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatagctgctaacgacgaaaactacaactacgctgacgcttcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1641223_sequence/1
type: Sequence
persistent identity: BBa K1641223 sequence
display id: BBa_K1641223_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatgctgctaacgacgaaaactacaactacgctgacgcttcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z