LOCUS BBa_K1641999 6049 bp DNA linear 29-Nov-2024 DEFINITION pAUR135 shuttle vector RFC10 optimized ACCESSION BBa_K1641999 VERSION BBa_K1641999.1 FEATURES Location/Qualifiers CDS 2822..4027 /label=AurR misc_feature 4434..5098 /label=GAL10 promoter misc_feature 2424..2478 /label=Lac operator CDS 481..1342 /label=AmpR misc_feature 1513..2100 /label=ori ORIGIN 1 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt 61 ctccttacgc atctgtgcgg tatttcacac cgcaaatggt gcactctcag tacaatctgc 121 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 301 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 361 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 421 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 481 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 541 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 601 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 661 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 721 cgtattgacg cccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 1021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 1081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 1141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 1201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 1261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 1321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 1381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 1441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 1501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 1561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 1621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 1681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 1741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 1801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 1861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 1921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 1981 gcgcacgagg gagcttccag ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc 2041 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 2101 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 2161 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 2221 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 2281 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 2341 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 2401 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 2461 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta 2521 gcttgcgtat tttttgcttc agttaccata gcgtaagaac actagcgact tttgttcgtg 2581 aaccaacaga gtaggatttc tactgctaca tctcttaggt agttggttag tccgatcgct 2641 cacttttggt tgttgttaag tacttcataa gtttatcctt ttcctttttc acactgagct 2701 actttgggta tagcttttgg cccaaggatc tttgaatttt ctccaaaagt actttatttt 2761 atatcctaca ggttgcggtt ttcatatttt aaaaagctag cttttaatca ttcctttgcg 2821 tatggcaaac cctttttcga gatggtttct atcagagaga cctccaaact gccatgtagc 2881 cgatttagaa acaagtttag atccccatca aacgttgttg aaggtgcaaa aatacaaacc 2941 cgctttaagc gactgggtgc attacatctt cttgggatcc atcatgctgt ttgtgttcat 3001 tactaatccc gcaccttgga tcttcaagat ccttttttat tgtttcttgg gcactttatt 3061 catcattcca gctacgtcac agtttttctt caatgccttg cccatcctaa catgggtggc 3121 gctgtatttc acttcatcgt actttccaga tgaccgcagg cctcctatta ctgtcaaagt 3181 gttaccagcg gtggaaacaa ttttatacgg cgacaattta agtgatattc ttgcaacatc 3241 gaccaattcc tttttggaca ttttagcatg gttaccgtac ggactatttc attatggggc 3301 cccatttgtc gttgctgcca tcttattcgt atttggtcca ccaactgttt tgcaaggtta 3361 tgcttttgca tttggttata tgaacctgtt tggtgttatc atgcaaaatg tctttccagc 3421 cgctccccca tggtataaaa ttctctatgg attgcaatca gccaactatg atatgcatgg 3481 ctcgcctggt ggattagcta gaattgataa gctactcggt attaatatgt atactacatg 3541 tttttcaaat tcctccgtca ttttcggtgc ttttccttca ctgcattccg ggtgtgctac 3601 tatggaagcc ctgtttttct gttattgttt tccaaaattg aagcccttgt ttattgctta 3661 tgtttgctgg ttatggtggt caactatgta tctgacacac cattattttg tagaccttat 3721 ggcaggttct gtgctgtcat acgttatttt ccagtacaca aagtacacac atttaccaat 3781 tgtagataca tctctttttt gcagatggtc atacacttca attgagaaat acgatatatc 3841 aaagagtgat ccattggctg ccgattcaaa cgatatcgaa agtgtccctt tgtccaactt 3901 ggaacttgac tttgatctta atatgactga tgaacccagt gtaagccctt cgttatttga 3961 tggatctact tctgtttctc gttcgtccgc cacgtctata acgtcactag gtgtaaagag 4021 ggcttaatga gtattttatc tgcaattacg gatacggttg gtcttatgta gatacatata 4081 aatatatatc tttttctttc tttttcttag tcaggattgt cgtttagcat aatatacatg 4141 tagtttattt aatcacatac cactgattat ctttagaatt ttataaattt ttgaaataaa 4201 tgggtggctt ttaatggtgt ctatgttaag tgaggctttt agaatgctct tcctgctttg 4261 tttattatat gtgtatgaaa gatatgtatg tatttacatg tgtttgtagc gtccccagtc 4321 aaaacctgtg cgctatacct aaatggattg ataatcttca ttcactaatt ctaaaataga 4381 cttcttcccc aaagaacggt gtaacgatga ggctctatcc aggactctag ggggtttttt 4441 ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat 4501 ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttatgcag 4561 tttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga ttaattaccc 4621 cagaaataag gctaaaaaac taatcgcatt atcatcctat ggttgttaat ttgattcgtt 4681 catttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc 4741 tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag 4801 gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac 4861 ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag cgtattactg 4921 aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac atttccacaa 4981 catataagta agattagata tggatatgta tatggatatg tatatggtgg taatgccatg 5041 taatatgatt attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat 5101 tcaaggaatt tcgacggatc aataacagtg tttgtggagc attttctgaa tacaataaac 5161 ccaaaacaga aacttccctt ttgtatcact gttctggaaa aggggtgggc ggtaataaag 5221 ctaatagggt gtgtccataa gtaatactga acttggaaat gtgcggcttt gcagcatttt 5281 gtctttctat aaaaatgtgt cgttcctttt tttcattttt tggcgcgtcg cctcggggtc 5341 gtatagaata tgcgtcactt ttaaaaataa gattgcagat cagggcaaaa caagtagcaa 5401 atcatagcaa gagaccctga tttttgtgac ataaatattt ttacttctgt gttaggttaa 5461 ctttttatgt aactgtaaat ggaatagagt tgaggggata gtgcccacaa gtcaatatgt 5521 ttattttgta aagttgaaag ataattattt ttatgctcag gtgattttgg tgttgaattt 5581 tctgtaatat taacataaga gtaatacatt gagtggttag tatatggtgt aaaagtggta 5641 taacgcatgt attaagagca gttatacaat atttggggcc gctgaatgag atatagatat 5701 taaaatgtgg ataatcatgg gctttatggg taaatggaac agggtataga ccactgaggc 5761 aagtgccgtg cataatgata tgagtgcatc tagtggcgaa cgtggcgaga aaggaaggga 5821 agaaagcgaa aggagcggct agaggatccc cgggtacgaa ttcgcggccg cttctagagt 5881 actagtagcg gccgctgcag aactcactgg ccgtcgtttt acaacgtcgt gactgtgaaa 5941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 6001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcct //