LOCUS       BBa_K1642009            3341 bp    DNA     linear       29-Nov-2024
DEFINITION  Expression of GFP under the control of Pdark
ACCESSION   BBa_K1642009
VERSION     BBa_K1642009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..561
                     /label=BBa_K1642005
     promoter        570..616
                     /label=BBa_K1026009
     RBS             625..644
                     /label=BBa_K1642004
     CDS             651..1370
                     /label=GFP protein
     CDS             651..1370
                     /label=BBa_E0040
     promoter        1379..1967
                     /label=PcpcB
     CDS             1968..2780
                     /label=KanR
     misc_feature    1379..2783
                     /label=BBa_K1642003
     misc_feature    2792..3341
                     /label=BBa_K1642006
ORIGIN
        1 cccggtatgg atggcaccga tgcggaatcc caacagattg cctttgacaa caatgtggcc
       61 tggaataacc tgggggattt gtccaccacc acccaacggg cctacacttc ggctattagc
      121 acagacacag tgcagagtgt ttatggcgtt aatctggaaa aaaacgataa cattcccatt
      181 gtttttgcgt ggcccatttt tcccaccacc cttaatccca cagattttca ggtaatgctt
      241 aacacggggg aaattgtcac cccggtgatc gcctctttga ttcccaacag tgaatacaac
      301 gaacggcaaa cggtagtaat tacgggcaat tttggtaatc gtttaacccc aggcacggag
      361 ggagcgattt atcccgtttc cgtaggcaca gtgttggaca gtactccttt ggaaatggtg
      421 ggacccaacg gcccggtcag tgcggtgggt attaccattg atagtctcaa cccctacgtg
      481 gccggcaatg gtcccaaaat tgtcgccgct aagttagacc gcttcagtga tgatgacttt
      541 ccgatttctt tacgattttc ttactagagc tttccgattt ctttacgatt ttctccgccc
      601 taggtattat gctagctact agagtataag taggagataa aaactactag atgcgtaaag
      661 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      721 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      781 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      841 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      901 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      961 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1021 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1081 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1141 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1201 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1261 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1321 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagaggt
     1381 ggtataaacg cagaaaggcc cctataccca cctgtagaga agagtccctg aatatcaaaa
     1441 tggtgggata aaaagctcaa aaaggaaagt aggctgtggt tccctaggca acagtcttcc
     1501 ctaccccact ggaaactaaa aaaacgagaa aagttcgcac cgaacatcaa ttgcataatt
     1561 ttagccctaa aacataagct gaacgaaact ggttgtcttc ccttcccaat ccaggacaat
     1621 ctgagaatcc cctgcaacat tacttaacaa aaaagcagga ataaaattaa caagatgtaa
     1681 cagacataag tcccatcacc gttgtataaa gttaactgtg ggattgcaaa agcattcaag
     1741 cctaggcgct gagctgtttg agcatcccgg tggcccttgt cgctgcctcc gtgtttctcc
     1801 ctggatttat ttaggtaata tctctcataa atccccgggt agttaacgaa agttaatgga
     1861 gatcagtaac aataactcta gggtcattac tttggactcc ctcagtttat ccgggggaat
     1921 tgtgtttaag aaaatcccaa ctcataaagt caagtaggag attaattatg agccatattc
     1981 aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg
     2041 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     2101 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2161 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2221 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
     2281 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2341 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgacc
     2401 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2461 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2521 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2581 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2641 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2701 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2761 tgatgctcga tgagtttttc taatactaga gccacaggtc atgaaattta tcagtacact
     2821 gacccagatg ggaaggtatt ttatgcttcc tttgctgccg ctgatgacca agccacggat
     2881 ttaaccacgg cgatcgccaa tcccacggcc atcgatttaa ttaacgccag gggatttacg
     2941 gcgggtagtt ccgtcaccgt atcgggttcc tacagtcggg aagccttttt tgatggatcc
     3001 atgggttttt atcgacttct ggacgataac ggtgcagtgc tagatccctt aacaggtggt
     3061 gtaatcaacc caggacaggt aggttatcaa gaagcagctt tggcagatag caatcgtttg
     3121 caagccactg gctccaccct aacggcagaa gacctagaaa ccagagcatt ttccttcaat
     3181 attttgggtg gcgagttgta tgcgccattt ttaacggtta atgacagtct ttccggtatt
     3241 aatcagactt attttgcctt tgggtcggcc aacccagatg gcatcagcca cagcacaaac
     3301 ttgggaccca acgtgattgg ttttgaagat tttctcggcg g
//