BBa_K1642003 1 PcpcB-Kana Kanamycin Resistance Cassette in Cyanobacteria 2015-08-14T11:00:00Z 2015-09-18T04:39:53Z 6803 Constitutively false false _2059_ 24423 24405 9 false Kana false Can Yin annotation2435670 1 KanR range2435670 1 590 1402 annotation2435669 1 PcpcB range2435669 1 1 589 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1026009 1 BBa_K1026009 A rationally designed promoter negative regulated by CcaR 2013-09-26T11:00:00Z 2015-05-08T01:08:46Z Synechocystissp. PCC 6803 By fusing G-box, a region that CcaR binds, with the constitutive promoter, BBa_J23107, we devised a promoter that is constitutive without active CcaR (the transcription factor in green light sensor system, CcaS-CcaR-PcpcG2), but be inhibited when CcaR is activated by green light. false false _1333_ 0 12098 9 Not in stock true We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2. So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. false Hongyi WU BBa_K1642005 1 Up Upstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T07:27:55Z Slr Slr false false _2059_ 24405 24405 9 false Slr false Can Yin BBa_K1642004 1 RBS SD sequence of cpcB 2015-08-14T11:00:00Z 2015-09-09T02:13:42Z RBS RBS false false _2059_ 24423 24405 9 false RBS false Can Yin BBa_K1642009 1 BBa_K1642009 Expression of GFP under the control of Pdark 2015-09-04T11:00:00Z 2015-09-08T02:00:14Z Pdark-gfp Pdark-gfp false false _2059_ 24405 24405 9 false Pdark-gfp false Can Yin component2443769 1 BBa_K1642005 component2443770 1 BBa_K1026009 component2443771 1 BBa_K1642004 component2443777 1 BBa_K1642006 component2443776 1 BBa_K1642003 component2443773 1 BBa_E0040 annotation2443777 1 BBa_K1642006 range2443777 1 2792 3341 annotation2443770 1 BBa_K1026009 range2443770 1 570 616 annotation2443769 1 BBa_K1642005 range2443769 1 1 561 annotation2443776 1 BBa_K1642003 range2443776 1 1379 2783 annotation2443771 1 BBa_K1642004 range2443771 1 625 644 annotation2443773 1 BBa_E0040 range2443773 1 651 1370 BBa_K1642006 1 Down Downstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T01:32:30Z Slr Slr false false _2059_ 24423 24405 9 false Slr false Can Yin BBa_K1642004_sequence 1 tataagtaggagataaaaac BBa_K1642005_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtga BBa_K1642003_sequence 1 gtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaa BBa_K1642006_sequence 1 ccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1026009_sequence 1 ctttccgatttctttacgattttctccgccctaggtattatgctagc BBa_K1642009_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgatgatgactttccgatttctttacgattttcttactagagctttccgatttctttacgattttctccgccctaggtattatgctagctactagagtataagtaggagataaaaactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatactagagccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z