LOCUS       BBa_K1642010            3744 bp    DNA     linear       26-Nov-2024
DEFINITION  Expression of Halorhodopsin under the control of PcpcG2
ACCESSION   BBa_K1642010
VERSION     BBa_K1642010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..530
                     /label=BBa_K1642005
     promoter        539..799
                     /label=BBa_K1642002
     RBS             808..827
                     /label=BBa_K1642004
     misc_feature    1707..1724
                     /label=His-tag
     CDS             834..1706
                     /label=Halorhodopsin
     CDS             834..1724
                     /label=BBa_K1642001
     stem_loop       1740..1759
                     /label=T7 TE
     misc_feature    1766..1766
                     /label=stop
     polyA_site      1760..1773
                     /label=polya
     misc_feature    1733..1773
                     /label=BBa_B0012
     terminator      1733..1773
                     /label=BBa_B0012
     promoter        1782..2370
                     /label=PcpcB
     CDS             2371..3183
                     /label=KanR
     misc_feature    1782..3186
                     /label=BBa_K1642003
     misc_feature    3195..3744
                     /label=BBa_K1642006
ORIGIN
        1 cccggtatgg atggcaccga tgcggaatcc caacagattg cctttgacaa caatgtggcc
       61 tggaataacc tgggggattt gtccaccacc acccaacggg cctacacttc ggctattagc
      121 acagacacag tgcagagtgt ttatggcgtt aatctggaaa aaaacgataa cattcccatt
      181 gtttttgcgt ggcccatttt tcccaccacc cttaatccca cagattttca ggtaatgctt
      241 aacacggggg aaattgtcac cccggtgatc gcctctttga ttcccaacag tgaatacaac
      301 gaacggcaaa cggtagtaat tacgggcaat tttggtaatc gtttaacccc aggcacggag
      361 ggagcgattt atcccgtttc cgtaggcaca gtgttggaca gtactccttt ggaaatggtg
      421 ggacccaacg gcccggtcag tgcggtgggt attaccattg atagtctcaa cccctacgtg
      481 gccggcaatg gtcccaaaat tgtcgccgct aagttagacc gcttcagtga tactagagtc
      541 ctccactaaa agaatactca tagcccattg tgcttttctc tatcaacctc agcttacctg
      601 aaggggtgaa caggtctggg ttaattcatg ttgcgaaatg taacagtttt agtcgcatca
      661 gctaactttc cgatttcttt acgattttct cccccttttc ttcaatttta ctttgttagg
      721 atcgcatttt taatgccaac acataccagt tattggctgg acattaaaca acttttaagt
      781 ttaattacta actttacatt actagagtat aagtaggaga taaaaactac tagatgactg
      841 aaaccttgcc ccccgttacc gagtccgccg ttgccttaca agccgaagtg acccagcggg
      901 aattgttcga atttgtgtta aatgatccct tgttggcttc ctccttgtat attaatattg
      961 ccttagccgg cttatccatt ttgttgtttg tgttcatgac ccggggcttg gatgatcctc
     1021 gggccaagtt gattgccgtg tccaccatct tagttcccgt ggtttccatt gcctcctaca
     1081 ccggcttggc cagtggcttg accatttccg tgttggagat gcctgccggc cattttgctg
     1141 aaggcagttc cgtgatgttg ggcggcgaag aggtggatgg cgtggtgacc atgtggggcc
     1201 ggtacttgac ctgggccttg tccaccccca tgatcttgtt ggccttgggc ttgttggccg
     1261 gttccaacgc cactaaattg ttcaccgcca tcacctttga catcgccatg tgcgtgaccg
     1321 gtttggccgc cgctttgacc acctcctccc acttgatgcg ctggttttgg tatgccatct
     1381 cctgcgcctg tttcttggtg gtgttgtaca tcttgttggt ggaatgggcc caagatgcca
     1441 aagccgccgg caccgccgat atgtttaaca ccttgaaatt attgaccgtg gtgatgtggt
     1501 tgggctaccc tattgtgtgg gccttgggcg tggaaggcat tgccgttttg cccgtgggtg
     1561 ttacctcctg gggctactcc tttttggata ttgtggccaa atacattttt gcctttttgt
     1621 tgttgaacta tttgacctcc aatgaatccg tggtgtccgg ctccattttg gatgttccct
     1681 ccgccagtgg cactcccgcc gatgatcatc atcatcacca tcattactag agtcacactg
     1741 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggtggtataa acgcagaaag
     1801 gcccctatac ccacctgtag agaagagtcc ctgaatatca aaatggtggg ataaaaagct
     1861 caaaaaggaa agtaggctgt ggttccctag gcaacagtct tccctacccc actggaaact
     1921 aaaaaaacga gaaaagttcg caccgaacat caattgcata attttagccc taaaacataa
     1981 gctgaacgaa actggttgtc ttcccttccc aatccaggac aatctgagaa tcccctgcaa
     2041 cattacttaa caaaaaagca ggaataaaat taacaagatg taacagacat aagtcccatc
     2101 accgttgtat aaagttaact gtgggattgc aaaagcattc aagcctaggc gctgagctgt
     2161 ttgagcatcc cggtggccct tgtcgctgcc tccgtgtttc tccctggatt tatttaggta
     2221 atatctctca taaatccccg ggtagttaac gaaagttaat ggagatcagt aacaataact
     2281 ctagggtcat tactttggac tccctcagtt tatccggggg aattgtgttt aagaaaatcc
     2341 caactcataa agtcaagtag gagattaatt atgagccata ttcaacggga aacgtcttgc
     2401 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2461 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     2521 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2581 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2641 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     2701 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2761 ttgcattcga ttcctgtttg taattgtcct tttaacagcg accgcgtatt tcgtctcgct
     2821 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2881 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2941 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3001 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3061 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3121 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3181 ttctaatact agagccacag gtcatgaaat ttatcagtac actgacccag atgggaaggt
     3241 attttatgct tcctttgctg ccgctgatga ccaagccacg gatttaacca cggcgatcgc
     3301 caatcccacg gccatcgatt taattaacgc caggggattt acggcgggta gttccgtcac
     3361 cgtatcgggt tcctacagtc gggaagcctt ttttgatgga tccatgggtt tttatcgact
     3421 tctggacgat aacggtgcag tgctagatcc cttaacaggt ggtgtaatca acccaggaca
     3481 ggtaggttat caagaagcag ctttggcaga tagcaatcgt ttgcaagcca ctggctccac
     3541 cctaacggca gaagacctag aaaccagagc attttccttc aatattttgg gtggcgagtt
     3601 gtatgcgcca tttttaacgg ttaatgacag tctttccggt attaatcaga cttattttgc
     3661 ctttgggtcg gccaacccag atggcatcag ccacagcaca aacttgggac ccaacgtgat
     3721 tggttttgaa gattttctcg gcgg
//