BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K1642005 1 Up Upstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T07:27:55Z Slr Slr false false _2059_ 24405 24405 9 false Slr false Can Yin BBa_K1642002 1 PcpcG2 PcpcG2 2015-08-14T11:00:00Z 2015-09-16T06:05:55Z Ref CcaR false false _2059_ 24405 24405 9 false Light false Can Yin BBa_K1642004 1 RBS SD sequence of cpcB 2015-08-14T11:00:00Z 2015-09-09T02:13:42Z RBS RBS false false _2059_ 24423 24405 9 false RBS false Can Yin BBa_K1642003 1 PcpcB-Kana Kanamycin Resistance Cassette in Cyanobacteria 2015-08-14T11:00:00Z 2015-09-18T04:39:53Z 6803 Constitutively false false _2059_ 24423 24405 9 false Kana false Can Yin annotation2435670 1 KanR range2435670 1 590 1402 annotation2435669 1 PcpcB range2435669 1 1 589 BBa_K1642010 1 PcpcG2-HR Expression of Halorhodopsin under the control of PcpcG2 2015-09-04T11:00:00Z 2015-09-17T04:27:48Z Pdark-HR Pdark-HR false false _2059_ 24405 24405 9 false Pdark-HR false Can Yin component2467204 1 BBa_B0012 component2467203 1 BBa_K1642001 component2467198 1 BBa_K1642005 component2467210 1 BBa_K1642003 component2467199 1 BBa_K1642002 component2467211 1 BBa_K1642006 component2467200 1 BBa_K1642004 annotation2467203 1 BBa_K1642001 range2467203 1 834 1724 annotation2467200 1 BBa_K1642004 range2467200 1 808 827 annotation2467204 1 BBa_B0012 range2467204 1 1733 1773 annotation2467199 1 BBa_K1642002 range2467199 1 539 799 annotation2467210 1 BBa_K1642003 range2467210 1 1782 3186 annotation2467211 1 BBa_K1642006 range2467211 1 3195 3744 annotation2467198 1 BBa_K1642005 range2467198 1 1 530 BBa_K1642006 1 Down Downstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T01:32:30Z Slr Slr false false _2059_ 24423 24405 9 false Slr false Can Yin BBa_K1642001 1 HR-His Codon-optimized Halorhodopsin with His-tag 2015-08-14T11:00:00Z 2015-09-09T02:14:36Z Synthesized pHR false false _2059_ 24423 24405 9 false restriction false Can Yin annotation2435710 1 His-tag range2435710 1 874 891 annotation2435721 1 Halorhodopsin range2435721 1 1 873 BBa_K1642001_sequence 1 atgactgaaaccttgccccccgttaccgagtccgccgttgccttacaagccgaagtgacccagcgggaattgttcgaatttgtgttaaatgatcccttgttggcttcctccttgtatattaatattgccttagccggcttatccattttgttgtttgtgttcatgacccggggcttggatgatcctcgggccaagttgattgccgtgtccaccatcttagttcccgtggtttccattgcctcctacaccggcttggccagtggcttgaccatttccgtgttggagatgcctgccggccattttgctgaaggcagttccgtgatgttgggcggcgaagaggtggatggcgtggtgaccatgtggggccggtacttgacctgggccttgtccacccccatgatcttgttggccttgggcttgttggccggttccaacgccactaaattgttcaccgccatcacctttgacatcgccatgtgcgtgaccggtttggccgccgctttgaccacctcctcccacttgatgcgctggttttggtatgccatctcctgcgcctgtttcttggtggtgttgtacatcttgttggtggaatgggcccaagatgccaaagccgccggcaccgccgatatgtttaacaccttgaaattattgaccgtggtgatgtggttgggctaccctattgtgtgggccttgggcgtggaaggcattgccgttttgcccgtgggtgttacctcctggggctactcctttttggatattgtggccaaatacatttttgcctttttgttgttgaactatttgacctccaatgaatccgtggtgtccggctccattttggatgttccctccgccagtggcactcccgccgatgatcatcatcatcaccatcat BBa_K1642004_sequence 1 tataagtaggagataaaaac BBa_K1642005_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtga BBa_K1642010_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgatactagagtcctccactaaaagaatactcatagcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttacattactagagtataagtaggagataaaaactactagatgactgaaaccttgccccccgttaccgagtccgccgttgccttacaagccgaagtgacccagcgggaattgttcgaatttgtgttaaatgatcccttgttggcttcctccttgtatattaatattgccttagccggcttatccattttgttgtttgtgttcatgacccggggcttggatgatcctcgggccaagttgattgccgtgtccaccatcttagttcccgtggtttccattgcctcctacaccggcttggccagtggcttgaccatttccgtgttggagatgcctgccggccattttgctgaaggcagttccgtgatgttgggcggcgaagaggtggatggcgtggtgaccatgtggggccggtacttgacctgggccttgtccacccccatgatcttgttggccttgggcttgttggccggttccaacgccactaaattgttcaccgccatcacctttgacatcgccatgtgcgtgaccggtttggccgccgctttgaccacctcctcccacttgatgcgctggttttggtatgccatctcctgcgcctgtttcttggtggtgttgtacatcttgttggtggaatgggcccaagatgccaaagccgccggcaccgccgatatgtttaacaccttgaaattattgaccgtggtgatgtggttgggctaccctattgtgtgggccttgggcgtggaaggcattgccgttttgcccgtgggtgttacctcctggggctactcctttttggatattgtggccaaatacatttttgcctttttgttgttgaactatttgacctccaatgaatccgtggtgtccggctccattttggatgttccctccgccagtggcactcccgccgatgatcatcatcatcaccatcattactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatactagagccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg BBa_K1642003_sequence 1 gtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaa BBa_K1642002_sequence 1 tcctccactaaaagaatactcatagcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttacat BBa_K1642006_sequence 1 ccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z