LOCUS       BBa_K1642011            3561 bp    DNA     linear       29-Nov-2024
DEFINITION  Expression of Halorhodopsin under the control of Pdrak
ACCESSION   BBa_K1642011
VERSION     BBa_K1642011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..561
                     /label=BBa_K1642005
     promoter        570..616
                     /label=BBa_K1026009
     RBS             625..644
                     /label=BBa_K1642004
     misc_feature    1524..1541
                     /label=His-tag
     CDS             651..1523
                     /label=Halorhodopsin
     CDS             651..1541
                     /label=BBa_K1642001
     stem_loop       1557..1576
                     /label=T7 TE
     misc_feature    1583..1583
                     /label=stop
     polyA_site      1577..1590
                     /label=polya
     misc_feature    1550..1590
                     /label=BBa_B0012
     terminator      1550..1590
                     /label=BBa_B0012
     promoter        1599..2187
                     /label=PcpcB
     CDS             2188..3000
                     /label=KanR
     misc_feature    1599..3003
                     /label=BBa_K1642003
     misc_feature    3012..3561
                     /label=BBa_K1642006
ORIGIN
        1 cccggtatgg atggcaccga tgcggaatcc caacagattg cctttgacaa caatgtggcc
       61 tggaataacc tgggggattt gtccaccacc acccaacggg cctacacttc ggctattagc
      121 acagacacag tgcagagtgt ttatggcgtt aatctggaaa aaaacgataa cattcccatt
      181 gtttttgcgt ggcccatttt tcccaccacc cttaatccca cagattttca ggtaatgctt
      241 aacacggggg aaattgtcac cccggtgatc gcctctttga ttcccaacag tgaatacaac
      301 gaacggcaaa cggtagtaat tacgggcaat tttggtaatc gtttaacccc aggcacggag
      361 ggagcgattt atcccgtttc cgtaggcaca gtgttggaca gtactccttt ggaaatggtg
      421 ggacccaacg gcccggtcag tgcggtgggt attaccattg atagtctcaa cccctacgtg
      481 gccggcaatg gtcccaaaat tgtcgccgct aagttagacc gcttcagtga tgatgacttt
      541 ccgatttctt tacgattttc ttactagagc tttccgattt ctttacgatt ttctccgccc
      601 taggtattat gctagctact agagtataag taggagataa aaactactag atgactgaaa
      661 ccttgccccc cgttaccgag tccgccgttg ccttacaagc cgaagtgacc cagcgggaat
      721 tgttcgaatt tgtgttaaat gatcccttgt tggcttcctc cttgtatatt aatattgcct
      781 tagccggctt atccattttg ttgtttgtgt tcatgacccg gggcttggat gatcctcggg
      841 ccaagttgat tgccgtgtcc accatcttag ttcccgtggt ttccattgcc tcctacaccg
      901 gcttggccag tggcttgacc atttccgtgt tggagatgcc tgccggccat tttgctgaag
      961 gcagttccgt gatgttgggc ggcgaagagg tggatggcgt ggtgaccatg tggggccggt
     1021 acttgacctg ggccttgtcc acccccatga tcttgttggc cttgggcttg ttggccggtt
     1081 ccaacgccac taaattgttc accgccatca cctttgacat cgccatgtgc gtgaccggtt
     1141 tggccgccgc tttgaccacc tcctcccact tgatgcgctg gttttggtat gccatctcct
     1201 gcgcctgttt cttggtggtg ttgtacatct tgttggtgga atgggcccaa gatgccaaag
     1261 ccgccggcac cgccgatatg tttaacacct tgaaattatt gaccgtggtg atgtggttgg
     1321 gctaccctat tgtgtgggcc ttgggcgtgg aaggcattgc cgttttgccc gtgggtgtta
     1381 cctcctgggg ctactccttt ttggatattg tggccaaata catttttgcc tttttgttgt
     1441 tgaactattt gacctccaat gaatccgtgg tgtccggctc cattttggat gttccctccg
     1501 ccagtggcac tcccgccgat gatcatcatc atcaccatca ttactagagt cacactggct
     1561 caccttcggg tgggcctttc tgcgtttata tactagaggt ggtataaacg cagaaaggcc
     1621 cctataccca cctgtagaga agagtccctg aatatcaaaa tggtgggata aaaagctcaa
     1681 aaaggaaagt aggctgtggt tccctaggca acagtcttcc ctaccccact ggaaactaaa
     1741 aaaacgagaa aagttcgcac cgaacatcaa ttgcataatt ttagccctaa aacataagct
     1801 gaacgaaact ggttgtcttc ccttcccaat ccaggacaat ctgagaatcc cctgcaacat
     1861 tacttaacaa aaaagcagga ataaaattaa caagatgtaa cagacataag tcccatcacc
     1921 gttgtataaa gttaactgtg ggattgcaaa agcattcaag cctaggcgct gagctgtttg
     1981 agcatcccgg tggcccttgt cgctgcctcc gtgtttctcc ctggatttat ttaggtaata
     2041 tctctcataa atccccgggt agttaacgaa agttaatgga gatcagtaac aataactcta
     2101 gggtcattac tttggactcc ctcagtttat ccgggggaat tgtgtttaag aaaatcccaa
     2161 ctcataaagt caagtaggag attaattatg agccatattc aacgggaaac gtcttgctct
     2221 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2281 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     2341 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2401 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2461 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
     2521 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2581 cattcgattc ctgtttgtaa ttgtcctttt aacagcgacc gcgtatttcg tctcgctcag
     2641 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2701 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2761 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2821 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2881 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2941 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3001 taatactaga gccacaggtc atgaaattta tcagtacact gacccagatg ggaaggtatt
     3061 ttatgcttcc tttgctgccg ctgatgacca agccacggat ttaaccacgg cgatcgccaa
     3121 tcccacggcc atcgatttaa ttaacgccag gggatttacg gcgggtagtt ccgtcaccgt
     3181 atcgggttcc tacagtcggg aagccttttt tgatggatcc atgggttttt atcgacttct
     3241 ggacgataac ggtgcagtgc tagatccctt aacaggtggt gtaatcaacc caggacaggt
     3301 aggttatcaa gaagcagctt tggcagatag caatcgtttg caagccactg gctccaccct
     3361 aacggcagaa gacctagaaa ccagagcatt ttccttcaat attttgggtg gcgagttgta
     3421 tgcgccattt ttaacggtta atgacagtct ttccggtatt aatcagactt attttgcctt
     3481 tgggtcggcc aacccagatg gcatcagcca cagcacaaac ttgggaccca acgtgattgg
     3541 ttttgaagat tttctcggcg g
//