BBa_K1026009 1 BBa_K1026009 A rationally designed promoter negative regulated by CcaR 2013-09-26T11:00:00Z 2015-05-08T01:08:46Z Synechocystissp. PCC 6803 By fusing G-box, a region that CcaR binds, with the constitutive promoter, BBa_J23107, we devised a promoter that is constitutive without active CcaR (the transcription factor in green light sensor system, CcaS-CcaR-PcpcG2), but be inhibited when CcaR is activated by green light. false false _1333_ 0 12098 9 Not in stock true We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2. So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. false Hongyi WU BBa_K1642005 1 Up Upstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T07:27:55Z Slr Slr false false _2059_ 24405 24405 9 false Slr false Can Yin BBa_K1642003 1 PcpcB-Kana Kanamycin Resistance Cassette in Cyanobacteria 2015-08-14T11:00:00Z 2015-09-18T04:39:53Z 6803 Constitutively false false _2059_ 24423 24405 9 false Kana false Can Yin annotation2435670 1 KanR range2435670 1 590 1402 annotation2435669 1 PcpcB range2435669 1 1 589 BBa_K1642004 1 RBS SD sequence of cpcB 2015-08-14T11:00:00Z 2015-09-09T02:13:42Z RBS RBS false false _2059_ 24423 24405 9 false RBS false Can Yin BBa_K1642001 1 HR-His Codon-optimized Halorhodopsin with His-tag 2015-08-14T11:00:00Z 2015-09-09T02:14:36Z Synthesized pHR false false _2059_ 24423 24405 9 false restriction false Can Yin annotation2435710 1 His-tag range2435710 1 874 891 annotation2435721 1 Halorhodopsin range2435721 1 1 873 BBa_K1642006 1 Down Downstream sequence from slr0168 2015-08-14T11:00:00Z 2015-09-16T01:32:30Z Slr Slr false false _2059_ 24423 24405 9 false Slr false Can Yin BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1642011 1 BBa_K1642011 Expression of Halorhodopsin under the control of Pdrak 2015-09-04T11:00:00Z 2015-09-18T04:50:50Z Pdark-HR Pdark-HR false false _2059_ 24423 24405 9 false Pdark-HR false Can Yin component2459048 1 BBa_K1642003 component2459041 1 BBa_K1642001 component2459049 1 BBa_K1642006 component2459037 1 BBa_K1026009 component2459038 1 BBa_K1642004 component2459042 1 BBa_B0012 component2459036 1 BBa_K1642005 annotation2459041 1 BBa_K1642001 range2459041 1 651 1541 annotation2459048 1 BBa_K1642003 range2459048 1 1599 3003 annotation2459037 1 BBa_K1026009 range2459037 1 570 616 annotation2459036 1 BBa_K1642005 range2459036 1 1 561 annotation2459038 1 BBa_K1642004 range2459038 1 625 644 annotation2459042 1 BBa_B0012 range2459042 1 1550 1590 annotation2459049 1 BBa_K1642006 range2459049 1 3012 3561 BBa_K1642001_sequence 1 atgactgaaaccttgccccccgttaccgagtccgccgttgccttacaagccgaagtgacccagcgggaattgttcgaatttgtgttaaatgatcccttgttggcttcctccttgtatattaatattgccttagccggcttatccattttgttgtttgtgttcatgacccggggcttggatgatcctcgggccaagttgattgccgtgtccaccatcttagttcccgtggtttccattgcctcctacaccggcttggccagtggcttgaccatttccgtgttggagatgcctgccggccattttgctgaaggcagttccgtgatgttgggcggcgaagaggtggatggcgtggtgaccatgtggggccggtacttgacctgggccttgtccacccccatgatcttgttggccttgggcttgttggccggttccaacgccactaaattgttcaccgccatcacctttgacatcgccatgtgcgtgaccggtttggccgccgctttgaccacctcctcccacttgatgcgctggttttggtatgccatctcctgcgcctgtttcttggtggtgttgtacatcttgttggtggaatgggcccaagatgccaaagccgccggcaccgccgatatgtttaacaccttgaaattattgaccgtggtgatgtggttgggctaccctattgtgtgggccttgggcgtggaaggcattgccgttttgcccgtgggtgttacctcctggggctactcctttttggatattgtggccaaatacatttttgcctttttgttgttgaactatttgacctccaatgaatccgtggtgtccggctccattttggatgttccctccgccagtggcactcccgccgatgatcatcatcatcaccatcat BBa_K1642004_sequence 1 tataagtaggagataaaaac BBa_K1642005_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtga BBa_K1642003_sequence 1 gtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaa BBa_K1642011_sequence 1 cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgatgatgactttccgatttctttacgattttcttactagagctttccgatttctttacgattttctccgccctaggtattatgctagctactagagtataagtaggagataaaaactactagatgactgaaaccttgccccccgttaccgagtccgccgttgccttacaagccgaagtgacccagcgggaattgttcgaatttgtgttaaatgatcccttgttggcttcctccttgtatattaatattgccttagccggcttatccattttgttgtttgtgttcatgacccggggcttggatgatcctcgggccaagttgattgccgtgtccaccatcttagttcccgtggtttccattgcctcctacaccggcttggccagtggcttgaccatttccgtgttggagatgcctgccggccattttgctgaaggcagttccgtgatgttgggcggcgaagaggtggatggcgtggtgaccatgtggggccggtacttgacctgggccttgtccacccccatgatcttgttggccttgggcttgttggccggttccaacgccactaaattgttcaccgccatcacctttgacatcgccatgtgcgtgaccggtttggccgccgctttgaccacctcctcccacttgatgcgctggttttggtatgccatctcctgcgcctgtttcttggtggtgttgtacatcttgttggtggaatgggcccaagatgccaaagccgccggcaccgccgatatgtttaacaccttgaaattattgaccgtggtgatgtggttgggctaccctattgtgtgggccttgggcgtggaaggcattgccgttttgcccgtgggtgttacctcctggggctactcctttttggatattgtggccaaatacatttttgcctttttgttgttgaactatttgacctccaatgaatccgtggtgtccggctccattttggatgttccctccgccagtggcactcccgccgatgatcatcatcatcaccatcattactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtggtataaacgcagaaaggcccctatacccacctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgtcttcccttcccaatccaggacaatctgagaatcccctgcaacattacttaacaaaaaagcaggaataaaattaacaagatgtaacagacataagtcccatcaccgttgtataaagttaactgtgggattgcaaaagcattcaagcctaggcgctgagctgtttgagcatcccggtggcccttgtcgctgcctccgtgtttctccctggatttatttaggtaatatctctcataaatccccgggtagttaacgaaagttaatggagatcagtaacaataactctagggtcattactttggactccctcagtttatccgggggaattgtgtttaagaaaatcccaactcataaagtcaagtaggagattaattatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgaccgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatactagagccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg BBa_K1642006_sequence 1 ccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg BBa_K1026009_sequence 1 ctttccgatttctttacgattttctccgccctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z