LOCUS       BBa_K1647001            1046 bp    DNA     linear       22-Dec-2024
DEFINITION  Pompc+Cl operon+RBS+GFP+terminator
ACCESSION   BBa_K1647001
VERSION     BBa_K1647001.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    117..159
                     /label=OR2
     misc_feature    124..137
                     /label=OR2
     misc_feature    146..159
                     /label=OR1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        118..123
                     /label=-35
     misc_feature    151..153
                     /label=start
     misc_feature    117..162
                     /label=BBa_R0052
     promoter        117..162
                     /label=BBa_R0052
     RBS             171..183
                     /label=RBS-3\Weak
     RBS             177..180
                     /label=RBS
     misc_feature    171..183
                     /label=BBa_B0032
     RBS             171..183
                     /label=BBa_B0032
     CDS             190..909
                     /label=GFP protein
     CDS             190..909
                     /label=BBa_E0040
     stem_loop       929..972
                     /label=stem_loop
     misc_feature    918..997
                     /label=BBa_B0010
     terminator      918..997
                     /label=BBa_B0010
     stem_loop       1013..1032
                     /label=T7 TE
     misc_feature    1039..1039
                     /label=stop
     polyA_site      1033..1046
                     /label=polya
     misc_feature    1006..1046
                     /label=BBa_B0012
     terminator      1006..1046
                     /label=BBa_B0012
     misc_feature    171..1046
                     /label=BBa_E0240
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagttga
      121 caaacaagat acattgtatg aaaatacaag aaagtttgtt gatactagag tcacacagga
      181 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      241 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      301 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      361 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      421 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      481 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      541 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      601 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      661 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      721 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      781 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      841 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
      901 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1021 ttcgggtggg cctttctgcg tttata
//