LOCUS BBa_K1647001 1046 bp DNA linear 22-Nov-2024 DEFINITION Pompc+Cl operon+RBS+GFP+terminator ACCESSION BBa_K1647001 VERSION BBa_K1647001.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 117..159 /label=OR2 misc_feature 124..137 /label=OR2 misc_feature 146..159 /label=OR1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 118..123 /label=-35 misc_feature 151..153 /label=start misc_feature 117..162 /label=BBa_R0052 promoter 117..162 /label=BBa_R0052 RBS 171..183 /label=RBS-3\Weak RBS 177..180 /label=RBS misc_feature 171..183 /label=BBa_B0032 RBS 171..183 /label=BBa_B0032 CDS 190..909 /label=GFP protein CDS 190..909 /label=BBa_E0040 stem_loop 929..972 /label=stem_loop misc_feature 918..997 /label=BBa_B0010 terminator 918..997 /label=BBa_B0010 stem_loop 1013..1032 /label=T7 TE misc_feature 1039..1039 /label=stop polyA_site 1033..1046 /label=polya misc_feature 1006..1046 /label=BBa_B0012 terminator 1006..1046 /label=BBa_B0012 misc_feature 171..1046 /label=BBa_E0240 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagttga 121 caaacaagat acattgtatg aaaatacaag aaagtttgtt gatactagag tcacacagga 181 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 241 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 301 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 361 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 421 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 481 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 541 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 601 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 661 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 721 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 781 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 841 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 901 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1021 ttcgggtggg cctttctgcg tttata //