LOCUS BBa_K1647006 3964 bp DNA linear 22-Nov-2024 DEFINITION J23100+B0034+Cph8+B0034+ho1+B0034+pcyA+B0010 ACCESSION BBa_K1647006 VERSION BBa_K1647006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 48..51 /label=conserved misc_feature 44..55 /label=BBa_B0034 CDS 64..2298 /label=cph8 (cph1-envZ-fusion) misc_feature 171..171 /label=g->a misc_feature 64..2298 /label=BBa_K909002 CDS 44..2298 /label=BBa_K909003 misc_feature 2311..2314 /label=conserved RBS 2307..2318 /label=BBa_B0034 misc_feature 3051..3075 /label=Help:Barcodes CDS 2325..3075 /label=BBa_I15008 misc_feature 3088..3091 /label=conserved RBS 3084..3095 /label=BBa_B0034 CDS 3102..3851 /label=PcyA misc_feature 3852..3876 /label=Help:Barcodes CDS 3102..3876 /label=BBa_I15009 stem_loop 3896..3939 /label=stem_loop misc_feature 3885..3964 /label=BBa_B0010 terminator 3885..3964 /label=BBa_B0010 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gagatggcca ccaccgtaca actcagcgac caatccctcc gtcagctaga aaccctcgcc 121 atccacaccg cccacctgat tcagccccac ggtttagtgg tggtcctgca agaaccagac 181 ctcaccatca gccaaattag cgccaactgc accggcattt tagggcgatc gccagaggat 241 ttgttgggca gaaccctagg ggaagtgttt gatagctttc agattgatcc catccagagt 301 cgcctaacgg ccggacaaat cagcagcctc aaccccagta aactttgggc gcgggtcatg 361 ggggacgact ttgtcatttt tgacggggtt tttcatcgca acagtgacgg tttattggta 421 tgtgaactcg agccagccta cacttccgat aatctgccct tcctcggttt ttatcacatg 481 gccaacgctg ccctgaatcg gttgcgccaa caagctaatc tacgggattt ctacgatgtt 541 attgtcgaag aagtccgccg tatgactggc tttgaccggg tgatgctata ccgctttgat 601 gaaaataacc acggtgatgt cattgccgaa gataaacggg atgatatgga accctatttg 661 ggcctgcact atcccgaatc ggatattccc caacccgccc gtcggctatt tatccacaac 721 cccattcgag taattcccga tgtttatggt gtggcggtgc ccctgacccc agcggttaac 781 cccagcacca accgagcggt ggatttaaca gaatccattc tgcgcagtgc gtaccattgc 841 cacttgacct atctgaaaaa tatgggggta ggagcgtctt taaccatttc cctaattaag 901 gacggccatc tctgggggct cattgcctgc caccatcaaa cccccaaagt aattcccttt 961 gaactgcgta aagcctgcga attttttggt cgggtggtgt ttagcaacat ttccgcccag 1021 gaagatacgg aaaccttcga ttaccgggtg cagctggcgg agcatgaagc ggttttattg 1081 gacaaaatga ccacggcggc ggattttgtc gaaggattaa ctaatcatcc cgatcgcctg 1141 ttgggattaa cgggctccca gggggcggcc atttgctttg gggaaaaatt gattttagta 1201 ggggaaaccc cggacgagaa agcagtgcaa tatttactgc aatggttgga gaatcgggaa 1261 gtgcaagacg ttttcttcac ctcttccctc tcacaaattt atcctgatgc agtgaatttt 1321 aaatccgtgg ccagtggctt attggccatt cccattgccc gtcacaactt tttgctctgg 1381 tttcgccctg aagtgttgca aacggttaat tggggcggtg acccaaatca tgcttacgaa 1441 gctacccagg aagacggtaa aatcgagctc catccccgcc aatcctttga cctctggaaa 1501 gaaattgtcc gactccaatc tttgccctgg caatcggtgg aaatccaaag tgccctggcc 1561 ctgaaaaagg cgatcgtcaa cctcattttg cgccaggcag aagaattgca tatggcggct 1621 ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag tcacgacttg 1681 cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca ggatggctat 1741 ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga gcagtttatc 1801 gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa tgcagtactc 1861 ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc gctttacccc 1921 ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc gaatatggtg 1981 gtcaacgccg cccgttatgg caatggctgg gtcaaagtca gcagcggaac ggagccgaat 2041 cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca acgtaagcac 2101 ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac gggattaggg 2161 ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct tggcaccagc 2221 gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg ggcgcagggc 2281 atgacaaaag aagggtaata ctagagaaag aggagaaata ctagatgagt gtcaacttag 2341 cttcccagtt gcgggaaggg acgaaaaaat cccactccat ggcggagaac gtcggctttg 2401 tcaaatgctt cctcaagggc gttgtcgaga aaaattccta ccgtaagctg gttggcaatc 2461 tctactttgt ctacagtgcc atggaagagg aaatggcaaa atttaaggac catcccatcc 2521 tcagccacat ttacttcccc gaactcaacc gcaaacaaag cctagagcaa gacctgcaat 2581 tctattacgg ctccaactgg cggcaagaag tgaaaatttc tgccgctggc caagcctatg 2641 tggaccgagt ccggcaagtg gccgctacgg cccctgaatt gttggtggcc cattcctaca 2701 cccgttacct gggggatctt tccggcggtc aaattctcaa gaaaattgcc caaaatgcca 2761 tgaatctcca cgatggtggc acagctttct atgaatttgc cgacattgat gacgaaaagg 2821 cttttaaaaa tacctaccgt caagctatga atgatctgcc cattgaccaa gccaccgccg 2881 aacggattgt ggatgaagcc aatgacgcct ttgccatgaa catgaaaatg ttcaacgaac 2941 ttgaaggcaa cctgatcaag gcgatcggca ttatggtgtt caacagcctc acccgtcgcc 3001 gcagtcaagg cagcaccgaa gttggcctcg ccacctccga aggctaataa cgctgatagt 3061 gctagtgtag atcgctacta gagaaagagg agaaatacta gatggccgtc actgatttaa 3121 gtttgaccaa ttcttccctg atgcctacgt tgaacccgat gattcaacag ttggccctgg 3181 cgatcgccgc tagttggcaa agtttacccc tcaagcccta tcaattgccg gaggatttgg 3241 gctacgtaga aggccgcctg gaaggggaaa agttagtgat tgaaaatcgg tgctaccaaa 3301 cgccccagtt tcgcaaaatg catttggagt tggccaaggt gggcaaaggg ttggatattc 3361 tccactgtgt aatgtttcct gagcctttat acggtctacc tttgtttggc tgtgacattg 3421 tggccggccc cggtggagta agtgcggcta ttgcggatct atcccccacc caaagcgatc 3481 gccaattgcc cgcagcgtac caaaaatcat tggcagagct aggccagcca gaatttgagc 3541 aacaacggga attgcccccc tggggagaaa tattttctga atattgttta ttcatccgtc 3601 ccagcaatgt cactgaagaa gaaagatttg tacaaagggt agtggacttt ttgcaaattc 3661 attgtcacca atccatcgtt gccgaaccct tgtctgaagc tcaaactttg gagcaccgtc 3721 aggggcaaat tcattactgc caacaacaac agaaaaatga taaaacccgt cgggtactgg 3781 aaaaagcttt tggggaagct tgggcggaac ggtatatgag ccaagtctta tttgatgtta 3841 tccaataata actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa 3901 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3961 tctc //