LOCUS       BBa_K1647008             967 bp    DNA     linear       25-Nov-2024
DEFINITION  J23100+Riborugulator+GFP
ACCESSION   BBa_K1647008
VERSION     BBa_K1647008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..83
                     /label=BBa_J01010
     RBS             92..104
                     /label=RBS-3\Weak
     RBS             98..101
                     /label=RBS
     misc_feature    92..104
                     /label=BBa_B0032
     RBS             92..104
                     /label=BBa_B0032
     CDS             111..830
                     /label=GFP protein
     CDS             111..830
                     /label=BBa_E0040
     stem_loop       850..893
                     /label=stem_loop
     misc_feature    839..918
                     /label=BBa_B0010
     terminator      839..918
                     /label=BBa_B0010
     stem_loop       934..953
                     /label=T7 TE
     misc_feature    960..960
                     /label=stop
     polyA_site      954..967
                     /label=polya
     misc_feature    927..967
                     /label=BBa_B0012
     terminator      927..967
                     /label=BBa_B0012
     misc_feature    92..967
                     /label=BBa_E0240
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaactaga atcacctctt
       61 ggatttgggt attaaagagg agatactaga gtcacacagg aaagtactag atgcgtaaag
      121 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      181 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      241 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      301 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      361 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      421 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      481 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      541 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      601 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      661 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      721 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      781 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      961 gtttata
//