BBa_K1648000 1 BBa_K1648000 Recombination Template for mamAB Operon 2015-07-06T11:00:00Z 2015-07-25T12:43:34Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of false false _2065_ 13322 26823 9 Not in stock false In order to form a homologous recombination template, we select 250 bp of each fragment and do the overlapping PCR ligate fragments together. false Leung King Pong annotation2433091 1 mamN 3' flanking sequence range2433091 1 251 500 annotation2433090 1 mamH 5' flanking sequence range2433090 1 1 250 annotation2433093 1 mamU 3' flanking sequence range2433093 1 771 1020 annotation2433092 1 mamO 5' flanking sequence range2433092 1 521 770 BBa_K1648000_sequence 1 atgacgggaatggaacctggcagatcagaagttgaggggcaccagcgcaacgccctttatttattgtcggcgttgtgcatggttttcatgactctcgtcgttgctattcagccgctttttttgcgaaacgttcttaatatccccttcgagactgccggggcagtcaatgccaatgtgcaggtggtgaccgaggtccttgatcttttcatctttgcctatcttggttacctgtcagatcgcattggccgggtggacaggcggcttggtgggcgctggcccttggcatcatggccggctcgtgtgccgcgctatcgggcgccaccgccggtgcgttggccatgaaccaatattccggcttcgtgaagcggcacccggaattggcttcggctgccgccgcgggattgcaattcacccatcgggaatatgtccgttggggattgccgctgatggggatttttttggtgttgtcgaccgtgtacatcgccgttctcgcaggatgaccgatgacgacaggaactcgatgattgaaattggcgagaccatgggtgatcagcccaccaacaaaatcgtcttttgcgagcggtcgtggaaagcgcctgtctccatcctggcgttcctgatcctagtgactttcgcctggggggcctatctcctcgacaactacgacgaggacgactacttccgtggtagcgacgatatgtcggtcggccaattcctggtccgcaacgtcgccatgcctgatgtgcagcggctgtactatacagtaccactggcggatccctgggggtttatgtggggcgcagcgccggaccggtcgggctcatggaattgggacttcaggccgctatggggcggtgggccagcaacgaagccttgttccagggtgagatgcactggctggaggttcaaaccgaacagcgcaagccgctgatttccattgatggcgaggtggagaaaatggaaggtcccttccgcttcgacattctgcccggagcgctttccatactggttccgaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z