BBa_K1648001 1 BBa_K1648001 Recombination Template for mamXY, mamGC and mms Operons 2015-07-13T11:00:00Z 2015-09-15T07:51:31Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island). false false _2065_ 26823 26823 9 false In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together. false Leung King Pong annotation2433095 1 ftsZ-like 3' flanking sequence range2433095 1 251 500 annotation2433096 1 mamG 5' flanking sequence range2433096 1 501 750 annotation2433098 1 mms6 5' flanking sequence range2433098 1 1001 1300 annotation2433099 1 mms48 3' flanking sequence range2433099 1 1301 1550 annotation2433094 1 mamY 5' flanking sequence range2433094 1 1 250 annotation2433097 1 mamC 3' flanking sequence range2433097 1 751 1000 BBa_K1648001_sequence 1 atgttgatgaactttgtcaacaatgtatcaaagacgattaacggaggagcccgcatcgtatatgtcggatcgttctcctgggctgtcttatcacttctttttgtcaccgctttcagtggctggaataatattttctcaatgctcccccacgagatttttattctagttctaaccatttcacttccgattgctctgattgttttgatcttcatgctctcgcagattgtgagaaccgtcgagagcgtgaagtgcgcgccgtcaaggccgccgaggcagccatctccaacccgctcttgggcgacacctccatcgcgggggccaagggagtgctgatcaacatcactggcggcatggacatgacgctgttcgaggtggacgaggccgccaaccgtatccgcaccgaagtggcgcccgacgccaatatcatcttcggatccactttcgacgagaagctggacggcaagatgcgggtctcggtggtcgccaccggtatcgcctgaatgatcaagggcatcgcgggagttggcggaaccgcccttggcgtcggtggcggagttgccgcccctccggtctctgccgctgctgtcggcagcaccttgctggccggcaagggggtgtgcctggggctggggcttggcctcggtgcttggggtcccgttcttctcggcgttgccggattggcttgcgccgcctccctatgtgattatctgaagaatcgcaaagcgcaggctgaggcctccgccgagcctgcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggcctgaatgcctgctcagatcgccaacggaatggtttgcccccctggggttccggtcggaaccaaggccgccgccctgggtgaaatggaacgcgaaggcgcgactgccaaggttggcgcgggcaaggttggcgcgggcaaggttggcgctgccaaggccggagccgcgcccgccgcggcgcagggggctggaaccaaggttgtcgcggcgcagggggctggaaccaaggttgtcgcggcgcagggcgctggggccaaggccgctgcggttggcgttgggaaggtcggcgccggcgccaaggctgtcaagggagcctggagggtcggcgcggtcggctgagatctggcggcggcgggagcatgaggcttcgctctctcctgcttgggtctgtggtgcttgtgctcgcgtggtggaggcgtgggcggcttgttgcccgtcctgcgctggtgtcgccaccattgagtggacgcggtcggttaaggccgaggaggcgttgctgccacccgccacaactgcgtcgagcatggagacgccgcgactgttccgcagtacatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z