LOCUS       BBa_K1648002            1237 bp    DNA     linear       25-Nov-2024
DEFINITION  Recombination Template for mamAB Operon with Promotor and 
            Terminator
ACCESSION   BBa_K1648002
VERSION     BBa_K1648002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..330
                     /label=mamH 5' flanking sequence
     misc_feature    331..580
                     /label=mamN 3' flanking sequence
     misc_feature    601..850
                     /label=mamO 5' flanking sequence
     misc_feature    851..1100
                     /label=mamU 3' flanking sequence
     misc_feature    81..1100
                     /label=BBa_K1648000
     stem_loop       1120..1163
                     /label=stem_loop
     misc_feature    1109..1188
                     /label=BBa_B0010
     terminator      1109..1188
                     /label=BBa_B0010
     stem_loop       1204..1223
                     /label=T7 TE
     misc_feature    1230..1230
                     /label=stop
     polyA_site      1224..1237
                     /label=polya
     misc_feature    1197..1237
                     /label=BBa_B0012
     terminator      1197..1237
                     /label=BBa_B0012
     terminator      1109..1237
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgacgggaa tggaacctgg cagatcagaa gttgaggggc
      121 accagcgcaa cgccctttat ttattgtcgg cgttgtgcat ggttttcatg actctcgtcg
      181 ttgctattca gccgcttttt ttgcgaaacg ttcttaatat ccccttcgag actgccgggg
      241 cagtcaatgc caatgtgcag gtggtgaccg aggtccttga tcttttcatc tttgcctatc
      301 ttggttacct gtcagatcgc attggccggg tggacaggcg gcttggtggg cgctggccct
      361 tggcatcatg gccggctcgt gtgccgcgct atcgggcgcc accgccggtg cgttggccat
      421 gaaccaatat tccggcttcg tgaagcggca cccggaattg gcttcggctg ccgccgcggg
      481 attgcaattc acccatcggg aatatgtccg ttggggattg ccgctgatgg ggattttttt
      541 ggtgttgtcg accgtgtaca tcgccgttct cgcaggatga ccgatgacga caggaactcg
      601 atgattgaaa ttggcgagac catgggtgat cagcccacca acaaaatcgt cttttgcgag
      661 cggtcgtgga aagcgcctgt ctccatcctg gcgttcctga tcctagtgac tttcgcctgg
      721 ggggcctatc tcctcgacaa ctacgacgag gacgactact tccgtggtag cgacgatatg
      781 tcggtcggcc aattcctggt ccgcaacgtc gccatgcctg atgtgcagcg gctgtactat
      841 acagtaccac tggcggatcc ctgggggttt atgtggggcg cagcgccgga ccggtcgggc
      901 tcatggaatt gggacttcag gccgctatgg ggcggtgggc cagcaacgaa gccttgttcc
      961 agggtgagat gcactggctg gaggttcaaa ccgaacagcg caagccgctg atttccattg
     1021 atggcgaggt ggagaaaatg gaaggtccct tccgcttcga cattctgccc ggagcgcttt
     1081 ccatactggt tccgaaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1141 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1201 actggctcac cttcgggtgg gcctttctgc gtttata
//