LOCUS       BBa_K1648003            1767 bp    DNA     linear       25-Nov-2024
DEFINITION  Recombination Template for mamXY,mamGC and mms Operons with 
            Promotor and Terminator
ACCESSION   BBa_K1648003
VERSION     BBa_K1648003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..330
                     /label=mamY 5' flanking sequence
     misc_feature    331..580
                     /label=ftsZ-like 3' flanking sequence
     misc_feature    581..830
                     /label=mamG 5' flanking sequence
     misc_feature    831..1080
                     /label=mamC 3' flanking sequence
     misc_feature    1081..1380
                     /label=mms6 5' flanking sequence
     misc_feature    1381..1630
                     /label=mms48 3' flanking sequence
     misc_feature    81..1630
                     /label=BBa_K1648001
     stem_loop       1650..1693
                     /label=stem_loop
     misc_feature    1639..1718
                     /label=BBa_B0010
     terminator      1639..1718
                     /label=BBa_B0010
     stem_loop       1734..1753
                     /label=T7 TE
     misc_feature    1760..1760
                     /label=stop
     polyA_site      1754..1767
                     /label=polya
     misc_feature    1727..1767
                     /label=BBa_B0012
     terminator      1727..1767
                     /label=BBa_B0012
     terminator      1639..1767
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttgatga actttgtcaa caatgtatca aagacgatta
      121 acggaggagc ccgcatcgta tatgtcggat cgttctcctg ggctgtctta tcacttcttt
      181 ttgtcaccgc tttcagtggc tggaataata ttttctcaat gctcccccac gagattttta
      241 ttctagttct aaccatttca cttccgattg ctctgattgt tttgatcttc atgctctcgc
      301 agattgtgag aaccgtcgag agcgtgaagt gcgcgccgtc aaggccgccg aggcagccat
      361 ctccaacccg ctcttgggcg acacctccat cgcgggggcc aagggagtgc tgatcaacat
      421 cactggcggc atggacatga cgctgttcga ggtggacgag gccgccaacc gtatccgcac
      481 cgaagtggcg cccgacgcca atatcatctt cggatccact ttcgacgaga agctggacgg
      541 caagatgcgg gtctcggtgg tcgccaccgg tatcgcctga atgatcaagg gcatcgcggg
      601 agttggcgga accgcccttg gcgtcggtgg cggagttgcc gcccctccgg tctctgccgc
      661 tgctgtcggc agcaccttgc tggccggcaa gggggtgtgc ctggggctgg ggcttggcct
      721 cggtgcttgg ggtcccgttc ttctcggcgt tgccggattg gcttgcgccg cctccctatg
      781 tgattatctg aagaatcgca aagcgcaggc tgaggcctcc gccgagcctg cacagaagcg
      841 gccatcgaca ccggcaagga agccgccggc gccgggcttg ccaccgcttt ctccgccgtc
      901 gccgccaccg ccgtcggcgg tgggttggtg gtctcgttgg gagccgccct aatcgccggc
      961 gtcgccgcca aatacgcctg ggacctgggt gtcgatttca tcgagaagga attgcgtcac
     1021 ggcaagtccg ccgaggcgac agcgtccgac gaagacattc tgagggaaga attggcctga
     1081 atgcctgctc agatcgccaa cggaatggtt tgcccccctg gggttccggt cggaaccaag
     1141 gccgccgccc tgggtgaaat ggaacgcgaa ggcgcgactg ccaaggttgg cgcgggcaag
     1201 gttggcgcgg gcaaggttgg cgctgccaag gccggagccg cgcccgccgc ggcgcagggg
     1261 gctggaacca aggttgtcgc ggcgcagggg gctggaacca aggttgtcgc ggcgcagggc
     1321 gctggggcca aggccgctgc ggttggcgtt gggaaggtcg gcgccggcgc caaggctgtc
     1381 aagggagcct ggagggtcgg cgcggtcggc tgagatctgg cggcggcggg agcatgaggc
     1441 ttcgctctct cctgcttggg tctgtggtgc ttgtgctcgc gtggtggagg cgtgggcggc
     1501 ttgttgcccg tcctgcgctg gtgtcgccac cattgagtgg acgcggtcgg ttaaggccga
     1561 ggaggcgttg ctgccacccg ccacaactgc gtcgagcatg gagacgccgc gactgttccg
     1621 cagtacatga tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1681 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1741 cttcgggtgg gcctttctgc gtttata
//