BBa_J13002 1 BBa_J13002 TetR repressed POPS/RIPS generator 2005-06-15T11:00:00Z 2015-08-31T04:08:29Z Released HQ 2013 -- No description -- false true _37_5_ 0 88 37 In stock false true Jeff Tabor component1535786 1 BBa_B0034 component1535778 1 BBa_R0040 annotation1535778 1 BBa_R0040 range1535778 1 1 54 annotation1535786 1 BBa_B0034 range1535786 1 63 74 BBa_K1648003 1 BBa_K1648003 Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator 2015-07-16T11:00:00Z 2015-09-16T08:39:15Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome. Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operons'''except the mamO operon is designed for '''homologous recombination'''. Part containing this template ([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) together with another part with mamO operon([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''. false false _2065_ 26823 26823 9 false In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamPAQRBSTU (parts of mamAB operon without the mamO operon) as flanking sequences. Fragments are joined via overlapping PCR. false Leung King Pong component2463289 1 BBa_J13002 component2463303 1 BBa_B0015 component2463296 1 BBa_K1648001 annotation2463289 1 BBa_J13002 range2463289 1 1 74 annotation2463296 1 BBa_K1648001 range2463296 1 81 1630 annotation2463303 1 BBa_B0015 range2463303 1 1639 1767 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1648001 1 BBa_K1648001 Recombination Template for mamXY, mamGC and mms Operons 2015-07-13T11:00:00Z 2015-09-15T07:51:31Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island). false false _2065_ 26823 26823 9 false In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together. false Leung King Pong annotation2433098 1 mms6 5' flanking sequence range2433098 1 1001 1300 annotation2433097 1 mamC 3' flanking sequence range2433097 1 751 1000 annotation2433096 1 mamG 5' flanking sequence range2433096 1 501 750 annotation2433099 1 mms48 3' flanking sequence range2433099 1 1301 1550 annotation2433095 1 ftsZ-like 3' flanking sequence range2433095 1 251 500 annotation2433094 1 mamY 5' flanking sequence range2433094 1 1 250 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J13002_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1648001_sequence 1 atgttgatgaactttgtcaacaatgtatcaaagacgattaacggaggagcccgcatcgtatatgtcggatcgttctcctgggctgtcttatcacttctttttgtcaccgctttcagtggctggaataatattttctcaatgctcccccacgagatttttattctagttctaaccatttcacttccgattgctctgattgttttgatcttcatgctctcgcagattgtgagaaccgtcgagagcgtgaagtgcgcgccgtcaaggccgccgaggcagccatctccaacccgctcttgggcgacacctccatcgcgggggccaagggagtgctgatcaacatcactggcggcatggacatgacgctgttcgaggtggacgaggccgccaaccgtatccgcaccgaagtggcgcccgacgccaatatcatcttcggatccactttcgacgagaagctggacggcaagatgcgggtctcggtggtcgccaccggtatcgcctgaatgatcaagggcatcgcgggagttggcggaaccgcccttggcgtcggtggcggagttgccgcccctccggtctctgccgctgctgtcggcagcaccttgctggccggcaagggggtgtgcctggggctggggcttggcctcggtgcttggggtcccgttcttctcggcgttgccggattggcttgcgccgcctccctatgtgattatctgaagaatcgcaaagcgcaggctgaggcctccgccgagcctgcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggcctgaatgcctgctcagatcgccaacggaatggtttgcccccctggggttccggtcggaaccaaggccgccgccctgggtgaaatggaacgcgaaggcgcgactgccaaggttggcgcgggcaaggttggcgcgggcaaggttggcgctgccaaggccggagccgcgcccgccgcggcgcagggggctggaaccaaggttgtcgcggcgcagggggctggaaccaaggttgtcgcggcgcagggcgctggggccaaggccgctgcggttggcgttgggaaggtcggcgccggcgccaaggctgtcaagggagcctggagggtcggcgcggtcggctgagatctggcggcggcgggagcatgaggcttcgctctctcctgcttgggtctgtggtgcttgtgctcgcgtggtggaggcgtgggcggcttgttgcccgtcctgcgctggtgtcgccaccattgagtggacgcggtcggttaaggccgaggaggcgttgctgccacccgccacaactgcgtcgagcatggagacgccgcgactgttccgcagtacatga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1648003_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttgatgaactttgtcaacaatgtatcaaagacgattaacggaggagcccgcatcgtatatgtcggatcgttctcctgggctgtcttatcacttctttttgtcaccgctttcagtggctggaataatattttctcaatgctcccccacgagatttttattctagttctaaccatttcacttccgattgctctgattgttttgatcttcatgctctcgcagattgtgagaaccgtcgagagcgtgaagtgcgcgccgtcaaggccgccgaggcagccatctccaacccgctcttgggcgacacctccatcgcgggggccaagggagtgctgatcaacatcactggcggcatggacatgacgctgttcgaggtggacgaggccgccaaccgtatccgcaccgaagtggcgcccgacgccaatatcatcttcggatccactttcgacgagaagctggacggcaagatgcgggtctcggtggtcgccaccggtatcgcctgaatgatcaagggcatcgcgggagttggcggaaccgcccttggcgtcggtggcggagttgccgcccctccggtctctgccgctgctgtcggcagcaccttgctggccggcaagggggtgtgcctggggctggggcttggcctcggtgcttggggtcccgttcttctcggcgttgccggattggcttgcgccgcctccctatgtgattatctgaagaatcgcaaagcgcaggctgaggcctccgccgagcctgcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggcctgaatgcctgctcagatcgccaacggaatggtttgcccccctggggttccggtcggaaccaaggccgccgccctgggtgaaatggaacgcgaaggcgcgactgccaaggttggcgcgggcaaggttggcgcgggcaaggttggcgctgccaaggccggagccgcgcccgccgcggcgcagggggctggaaccaaggttgtcgcggcgcagggggctggaaccaaggttgtcgcggcgcagggcgctggggccaaggccgctgcggttggcgttgggaaggtcggcgccggcgccaaggctgtcaagggagcctggagggtcggcgcggtcggctgagatctggcggcggcgggagcatgaggcttcgctctctcctgcttgggtctgtggtgcttgtgctcgcgtggtggaggcgtgggcggcttgttgcccgtcctgcgctggtgtcgccaccattgagtggacgcggtcggttaaggccgaggaggcgttgctgccacccgccacaactgcgtcgagcatggagacgccgcgactgttccgcagtacatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z