Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome
Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome. Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operons'''except the mamO operon is designed for '''homologous recombination'''. Part containing this template ([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) together with another part with mamO operon([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamPAQRBSTU (parts of mamAB operon without the mamO operon) as flanking sequences. Fragments are joined via overlapping PCR.
Double terminator consisting of BBa_B0010 and BBa_B0012
Recombination Template for mamXY, mamGC and mms Operons
This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island).
In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Conversion of the iGEM parts registry to SBOL2.1