BBa_K1648003
type: ComponentDefinition
persistent identity: BBa K1648003
display id: BBa_K1648003
version: 1
was generated by: iGEM to SBOL conversion
description: Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator
created: 2015-07-16T11:00:00Z
modified: 2015-09-16T08:39:15Z
mutable provenance: Magnetospirillum gryphiswaldense MSR-1 v2, complete genome
owned by: james
owned by: myers
mutable description: Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome. Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operons'''except the mamO operon is designed for '''homologous recombination'''. Part containing this template ([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) together with another part with mamO operon([http://parts.igem.org/Part:BBa_K164XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
discontinued: false
dominant: false
experience: experience/None
group u list: _2065_
m user id: 26823
owner id: 26823
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamPAQRBSTU (parts of mamAB operon without the mamO operon) as flanking sequences. Fragments are joined via overlapping PCR.
star: false
creator: Leung King Pong
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1648001
component: BBa_J13002
sequence annotation: BBa_J13002
sequence annotation: BBa_K1648001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1648003_sequence/1

BBa_B0015
type: Component
persistent identity: component2463303
display id: component2463303
version: 1
top level: BBa_K1648003
definition: BBa_B0015
access: public

BBa_K1648001
type: Component
persistent identity: component2463296
display id: component2463296
version: 1
top level: BBa_K1648003
definition: BBa_K1648001
access: public

BBa_J13002
type: Component
persistent identity: component2463289
display id: component2463289
version: 1
top level: BBa_K1648003
definition: BBa_J13002
access: public

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2463289
display id: annotation2463289
version: 1
top level: BBa_K1648003
location: https://synbiohub.org/public/igem/BBa_K1648003/annotation2463289/range2463289/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_K1648003/annotation2463289/range2463289/1
type: Range
persistent identity: range2463289
display id: range2463289
version: 1
top level: BBa_K1648003
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_K1648001
type: SequenceAnnotation
persistent identity: annotation2463296
display id: annotation2463296
version: 1
top level: BBa_K1648003
location: https://synbiohub.org/public/igem/BBa_K1648003/annotation2463296/range2463296/1
component: BBa_K1648001

https://synbiohub.org/public/igem/BBa_K1648003/annotation2463296/range2463296/1
type: Range
persistent identity: range2463296
display id: range2463296
version: 1
top level: BBa_K1648003
direction: direction/reverse
start: 81
end: 1630
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2463303
display id: annotation2463303
version: 1
top level: BBa_K1648003
location: https://synbiohub.org/public/igem/BBa_K1648003/annotation2463303/range2463303/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1648003/annotation2463303/range2463303/1
type: Range
persistent identity: range2463303
display id: range2463303
version: 1
top level: BBa_K1648003
direction: direction/reverse
start: 1639
end: 1767
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J13002
type: ComponentDefinition
persistent identity: BBa J13002
display id: BBa_J13002
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressed POPS/RIPS generator
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13002_sequence/1

BBa_B0034
type: Component
persistent identity: component1535786
display id: component1535786
version: 1
top level: BBa_J13002
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1535778
display id: component1535778
version: 1
top level: BBa_J13002
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1535778
display id: annotation1535778
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
type: Range
persistent identity: range1535778
display id: range1535778
version: 1
top level: BBa_J13002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1535786
display id: annotation1535786
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
type: Range
persistent identity: range1535786
display id: range1535786
version: 1
top level: BBa_J13002
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_K1648001
type: ComponentDefinition
persistent identity: BBa K1648001
display id: BBa_K1648001
version: 1
was generated by: iGEM to SBOL conversion
description: Recombination Template for mamXY, mamGC and mms Operons
created: 2015-07-13T11:00:00Z
modified: 2015-09-15T07:51:31Z
mutable provenance: Magnetospirillum gryphiswaldense MSR-1 v2, complete genome
owned by: james
owned by: myers
mutable description: This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island).
discontinued: false
dominant: false
experience: experience/None
group u list: _2065_
m user id: 26823
owner id: 26823
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together.
star: false
creator: Leung King Pong
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: mamY 5' flanking sequence
sequence annotation: ftsZ-like 3' flanking sequence
sequence annotation: mamG 5' flanking sequence
sequence annotation: mamC 3' flanking sequence
sequence annotation: mms6 5' flanking sequence
sequence annotation: mms48 3' flanking sequence
sequence: https://synbiohub.org/public/igem/BBa_K1648001_sequence/1

mamY 5' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433094
display id: annotation2433094
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433094/range2433094/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433094/range2433094/1
type: Range
persistent identity: range2433094
display id: range2433094
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 1
end: 250
orientation: inline

ftsZ-like 3' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433095
display id: annotation2433095
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433095/range2433095/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433095/range2433095/1
type: Range
persistent identity: range2433095
display id: range2433095
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 251
end: 500
orientation: inline

mamG 5' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433096
display id: annotation2433096
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433096/range2433096/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433096/range2433096/1
type: Range
persistent identity: range2433096
display id: range2433096
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 501
end: 750
orientation: inline

mamC 3' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433097
display id: annotation2433097
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433097/range2433097/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433097/range2433097/1
type: Range
persistent identity: range2433097
display id: range2433097
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 751
end: 1000
orientation: inline

mms6 5' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433098
display id: annotation2433098
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433098/range2433098/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433098/range2433098/1
type: Range
persistent identity: range2433098
display id: range2433098
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 1001
end: 1300
orientation: inline

mms48 3' flanking sequence
type: SequenceAnnotation
persistent identity: annotation2433099
display id: annotation2433099
version: 1
top level: BBa_K1648001
location: https://synbiohub.org/public/igem/BBa_K1648001/annotation2433099/range2433099/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1648001/annotation2433099/range2433099/1
type: Range
persistent identity: range2433099
display id: range2433099
version: 1
top level: BBa_K1648001
direction: direction/forward
start: 1301
end: 1550
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K1648003_sequence/1
type: Sequence
persistent identity: BBa K1648003 sequence
display id: BBa_K1648003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttgatgaactttgtcaacaatgtatcaaagacgattaacggaggagcccgcatcgtatatgtcggatcgttctcctgggctgtcttatcacttctttttgtcaccgctttcagtggctggaataatattttctcaatgctcccccacgagatttttattctagttctaaccatttcacttccgattgctctgattgttttgatcttcatgctctcgcagattgtgagaaccgtcgagagcgtgaagtgcgcgccgtcaaggccgccgaggcagccatctccaacccgctcttgggcgacacctccatcgcgggggccaagggagtgctgatcaacatcactggcggcatggacatgacgctgttcgaggtggacgaggccgccaaccgtatccgcaccgaagtggcgcccgacgccaatatcatcttcggatccactttcgacgagaagctggacggcaagatgcgggtctcggtggtcgccaccggtatcgcctgaatgatcaagggcatcgcgggagttggcggaaccgcccttggcgtcggtggcggagttgccgcccctccggtctctgccgctgctgtcggcagcaccttgctggccggcaagggggtgtgcctggggctggggcttggcctcggtgcttggggtcccgttcttctcggcgttgccggattggcttgcgccgcctccctatgtgattatctgaagaatcgcaaagcgcaggctgaggcctccgccgagcctgcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggcctgaatgcctgctcagatcgccaacggaatggtttgcccccctggggttccggtcggaaccaaggccgccgccctgggtgaaatggaacgcgaaggcgcgactgccaaggttggcgcgggcaaggttggcgcgggcaaggttggcgctgccaaggccggagccgcgcccgccgcggcgcagggggctggaaccaaggttgtcgcggcgcagggggctggaaccaaggttgtcgcggcgcagggcgctggggccaaggccgctgcggttggcgttgggaaggtcggcgccggcgccaaggctgtcaagggagcctggagggtcggcgcggtcggctgagatctggcggcggcgggagcatgaggcttcgctctctcctgcttgggtctgtggtgcttgtgctcgcgtggtggaggcgtgggcggcttgttgcccgtcctgcgctggtgtcgccaccattgagtggacgcggtcggttaaggccgaggaggcgttgctgccacccgccacaactgcgtcgagcatggagacgccgcgactgttccgcagtacatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13002_sequence/1
type: Sequence
persistent identity: BBa J13002 sequence
display id: BBa_J13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1648001_sequence/1
type: Sequence
persistent identity: BBa K1648001 sequence
display id: BBa_K1648001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgatgaactttgtcaacaatgtatcaaagacgattaacggaggagcccgcatcgtatatgtcggatcgttctcctgggctgtcttatcacttctttttgtcaccgctttcagtggctggaataatattttctcaatgctcccccacgagatttttattctagttctaaccatttcacttccgattgctctgattgttttgatcttcatgctctcgcagattgtgagaaccgtcgagagcgtgaagtgcgcgccgtcaaggccgccgaggcagccatctccaacccgctcttgggcgacacctccatcgcgggggccaagggagtgctgatcaacatcactggcggcatggacatgacgctgttcgaggtggacgaggccgccaaccgtatccgcaccgaagtggcgcccgacgccaatatcatcttcggatccactttcgacgagaagctggacggcaagatgcgggtctcggtggtcgccaccggtatcgcctgaatgatcaagggcatcgcgggagttggcggaaccgcccttggcgtcggtggcggagttgccgcccctccggtctctgccgctgctgtcggcagcaccttgctggccggcaagggggtgtgcctggggctggggcttggcctcggtgcttggggtcccgttcttctcggcgttgccggattggcttgcgccgcctccctatgtgattatctgaagaatcgcaaagcgcaggctgaggcctccgccgagcctgcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggcctgaatgcctgctcagatcgccaacggaatggtttgcccccctggggttccggtcggaaccaaggccgccgccctgggtgaaatggaacgcgaaggcgcgactgccaaggttggcgcgggcaaggttggcgcgggcaaggttggcgctgccaaggccggagccgcgcccgccgcggcgcagggggctggaaccaaggttgtcgcggcgcagggggctggaaccaaggttgtcgcggcgcagggcgctggggccaaggccgctgcggttggcgttgggaaggtcggcgccggcgccaaggctgtcaagggagcctggagggtcggcgcggtcggctgagatctggcggcggcgggagcatgaggcttcgctctctcctgcttgggtctgtggtgcttgtgctcgcgtggtggaggcgtgggcggcttgttgcccgtcctgcgctggtgtcgccaccattgagtggacgcggtcggttaaggccgaggaggcgttgctgccacccgccacaactgcgtcgagcatggagacgccgcgactgttccgcagtacatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z