BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1648046 1 oprF* Mutated AvOprF 2015-07-24T11:00:00Z 2015-09-17T11:30:39Z Genomic DNA of Magnetospirillum gryphiswaldense. Second half of the mamAB operon from Magnetospirillum gryphiswaldense. mamAB operon is the minimal requirement for magnetosome biogenesis in M. gryphiswaldense [Citation]. false false _2065_ 27095 13322 9 false false Leung King Pong annotation2467919 1 B0015 range2467919 1 1085 1213 annotation2468717 1 C->S range2468717 1 621 623 annotation2467913 1 oprF K189G C->S range2467913 1 21 1076 annotation2468719 1 C->S range2468719 1 666 668 annotation2467897 1 RBS range2467897 1 1 14 annotation2468720 1 C->S range2468720 1 708 710 annotation2468718 1 C->S range2468718 1 648 650 annotation2468716 1 K->C range2468716 1 585 587 BBa_J13002 1 BBa_J13002 TetR repressed POPS/RIPS generator 2005-06-15T11:00:00Z 2015-08-31T04:08:29Z Released HQ 2013 -- No description -- false true _37_5_ 0 88 37 In stock false true Jeff Tabor component1535786 1 BBa_B0034 component1535778 1 BBa_R0040 annotation1535786 1 BBa_B0034 range1535786 1 63 74 annotation1535778 1 BBa_R0040 range1535778 1 1 54 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1648008 1 BBa_K1648008 mamAB expression cascade 2015-09-15T11:00:00Z 2015-09-16T09:22:12Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome the most crucial genome for forming magnetosome false false _2065_ 26823 26823 9 false the most crucial genome for forming magnetosome false Leung King Pong component2463694 1 BBa_K1648046 component2463701 1 BBa_B0015 component2463684 1 BBa_K1648045 component2463675 1 BBa_J13002 annotation2463684 1 BBa_K1648045 range2463684 1 83 9094 annotation2463694 1 BBa_K1648046 range2463694 1 9101 16581 annotation2463701 1 BBa_B0015 range2463701 1 16590 16718 annotation2463675 1 BBa_J13002 range2463675 1 1 74 BBa_K1648045 1 AvOprF AvOprF 2015-05-20T11:00:00Z 2015-09-18T01:18:43Z Synthesis. SH3 ligand interacts with SH3 domain. false false _2065_ 27095 13322 9 No part sequence false / false Leung King Pong annotation2464381 1 AvOprF range2464381 1 1 1056 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J13002_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa BBa_K1648046_sequence 1 agaaagaggagaaatactagatgaaactgaaaaacaccttgggcgccattattggctcgatggtcgccgcatcttcgatcagcgcgctggctcagggccagggcgcagttgaggtggaagctttcgccaagcactggttcagcgatgagagttcgcgcgacttcgaaaacgatgaagccgaactctatggtgcatcgatcggttattttttgaccgatgatgtggagctggtcttgtcgcatggcgtttacagggacgtcactaccgatgagccgattggcgacggcgggcacaaggacatcaagggtgaactgacctccatcgatgccctctatcacttcggcgagcccggcgcaggccttcgtccctacgtttccggcggcttcgcccaccagagcatcggccaggcccgtggcggccgtaacaacagcaccctggccaacgtcggcgccggtctgaagtactacttcaccgacatgctttacgcccgggccggtctcgaaggcatgtacaacttcgacgatgatcagaccgactggcaggctggcgtgggtatcggtctgaacttcggtggcagcggcggggggcagcaacccgttgcggagccggttgccgaggtctccgccgacgtcgacaacgatggcgtctccgacaatgtcgaccgctccccggatactcccgccaacaccaccgtgaatgccgagggctccaccgccgtcgccgaggtggtgcgtgtcgagctggacgtcaagttcgacttcgacaaggcgcaggtcaaggaagaaagctatgccgacatcaagaacctggccgacttcatgaagcagtatccgcagaccaacactaccgtggaaggtcacaccgactccgtcggtacggatgcctacaaccagaagctgtccgagcgccgtgccaatgcggtgcgtgatgttctggtcaacgagtatggcgtgcaaggcagccgcgtagattccgtcggttacggcgaatcccgtccggtggccgacaacgctaccgagtccggtcgcgctatcaaccgtcgcgtggaagcggaagtggaagcccaagcccagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1648008_sequence 1 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aatgccgagctttacgccaagtccgttctggtgggggggcggacatgcaagttgcgcgatgacgagggtttggcgctgtggatgaccgtgcagtgagccatatgggacagagagggtgaacatgggtacgccagggggcggccgtcgctggatgaccttgatctcgatcaccttgctgatggtggtcggactgggactctattgggatgagctgtccctctccgccggcatctcccccgccacatcgccccgtcgggcggaggggcttttgttggggcggctgcccttgcccatggagccttcgattctgtcgccgctggagcatctcattgagccgccgcttcagtacaagctgatgaccattcgtcatatcccgccggtaatgccggggacaggcatgccccatccctatgtgggggattgcatccaatgccatctgatggtcggtggccccgctgccggatcacagttcaagacgccctatggcgccgtactggaaaacctgtcgcgggtccgcaaactggggcctcccattcttcccacgacgcgccagccgcatccgcctgccgggcgctgcattaagtgccatgacattgtggtcaaggtgcctgtggaaaagaagtccggcattaaatggctgttgtaagcgccggtgtcgtccttcgctgaacaggggctgtgtggaagatgcggatcgccgcaatcatcaatgcgcgggccggaaccgtgcttcggatgtcgccctccgccgtgacggagcgactatcggtcgtttggggctcgcttgggcatgatgccgcgatcattcttgccgaaggtaaggacatggggcggatggtccggaaggcttgccgcgatcccgacatacgggcgatcatcatcggcggcggtgacggatctctgtcacgggctctcgaacatgtgctcgcaagcggcaaatcgctgggtgtcctgcccctgggcaccatgaactacatggcgcgtcagatcggaatgccgttggatctggcccaggcggcggtggccttggcgggagccgtccagactcccatggatgtgggacgggtcaatgatcgttatttccttatccgagcctgtttcggcgcctttcccgaattcattcagtcgcgcgaccgggtccggcgcaaggggggaagctttcttgaaggcgccctggccggattgagcggagtggcgcggcgctatcggattgtggaggcggaattgtccagcccaggggggcgcgcccggatcgccacaagcttcctgatgatttccaacaatctgtgccgggactccgacccgttcctgctggagcgtgaacggatggatggcggatccctgggggtttatgtggggcgcagcgccggaccggtcgggctcatggaattgggacttcaggccgctatggggcggtgggccagcaacgaagccttgttccagggtgagatgcactggctggaggttcaaaccgaacagcgcaagccgctgatttccattgatggcgaggtggagaaaatggaaggtcccttccgcttcgacattctgcccggagcgctttccatactggttccgaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1648045_sequence 1 atgaaactgaaaaacaccttgggcgccattattggctcgatggtcgccgcatcttcgatcagcgcgctggctcagggccagggcgcagttgaggtggaagctttcgccaagcactggttcagcgatgagagttcgcgcgacttcgaaaacgatgaagccgaactctatggtgcatcgatcggttattttttgaccgatgatgtggagctggtcttgtcgcatggcgtttacagggacgtcactaccgatgagccgattggcgacggcgggcacaaggacatcaagggtgaactgacctccatcgatgccctctatcacttcggcgagcccggcgcaggccttcgtccctacgtttccggcggcttcgcccaccagagcatcggccaggcccgtggcggccgtaacaacagcaccctggccaacgtcggcgccggtctgaagtactacttcaccgacatgctttacgcccgggccggtctcgaaggcatgtacaacttcgacgatgatcagaccgactggcaggctggcgtgggtatcggtctgaacttcggtggcagcggcgggaagcagcaacccgttgcggagccggttgccgaggtctgtgccgacgtcgacaacgatggcgtctgcgacaatgtcgaccgctgcccggatactcccgccaacaccaccgtgaatgccgagggctgcaccgccgtcgccgaggtggtgcgtgtcgagctggacgtcaagttcgacttcgacaaggcgcaggtcaaggaagaaagctatgccgacatcaagaacctggccgacttcatgaagcagtatccgcagaccaacactaccgtggaaggtcacaccgactccgtcggtacggatgcctacaaccagaagctgtccgagcgccgtgccaatgcggtgcgtgatgttctggtcaacgagtatggcgtgcaaggcagccgcgtagattccgtcggttacggcgaatcccgtccggtggccgacaacgctaccgagtccggtcgcgctatcaaccgtcgcgtggaagcggaagtggaagcccaagcccagtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z