LOCUS       BBa_K1648049            1273 bp    DNA     linear       17-May-2024
DEFINITION  Mutated Av oprF induced by J13002
ACCESSION   BBa_K1648049
VERSION     BBa_K1648049.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1136
                     /label=AvOprF
     CDS             81..1136
                     /label=BBa_K1648045
     misc_feature    63..1136
                     /label=BBa_K1648047
     stem_loop       1156..1199
                     /label=stem_loop
     misc_feature    1145..1224
                     /label=BBa_B0010
     terminator      1145..1224
                     /label=BBa_B0010
     stem_loop       1240..1259
                     /label=T7 TE
     misc_feature    1266..1266
                     /label=stop
     polyA_site      1260..1273
                     /label=polya
     misc_feature    1233..1273
                     /label=BBa_B0012
     terminator      1233..1273
                     /label=BBa_B0012
     terminator      1145..1273
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaactga aaaacacctt gggcgccatt attggctcga
      121 tggtcgccgc atcttcgatc agcgcgctgg ctcagggcca gggcgcagtt gaggtggaag
      181 ctttcgccaa gcactggttc agcgatgaga gttcgcgcga cttcgaaaac gatgaagccg
      241 aactctatgg tgcatcgatc ggttattttt tgaccgatga tgtggagctg gtcttgtcgc
      301 atggcgttta cagggacgtc actaccgatg agccgattgg cgacggcggg cacaaggaca
      361 tcaagggtga actgacctcc atcgatgccc tctatcactt cggcgagccc ggcgcaggcc
      421 ttcgtcccta cgtttccggc ggcttcgccc accagagcat cggccaggcc cgtggcggcc
      481 gtaacaacag caccctggcc aacgtcggcg ccggtctgaa gtactacttc accgacatgc
      541 tttacgcccg ggccggtctc gaaggcatgt acaacttcga cgatgatcag accgactggc
      601 aggctggcgt gggtatcggt ctgaacttcg gtggcagcgg cgggaagcag caacccgttg
      661 cggagccggt tgccgaggtc tgtgccgacg tcgacaacga tggcgtctgc gacaatgtcg
      721 accgctgccc ggatactccc gccaacacca ccgtgaatgc cgagggctgc accgccgtcg
      781 ccgaggtggt gcgtgtcgag ctggacgtca agttcgactt cgacaaggcg caggtcaagg
      841 aagaaagcta tgccgacatc aagaacctgg ccgacttcat gaagcagtat ccgcagacca
      901 acactaccgt ggaaggtcac accgactccg tcggtacgga tgcctacaac cagaagctgt
      961 ccgagcgccg tgccaatgcg gtgcgtgatg ttctggtcaa cgagtatggc gtgcaaggca
     1021 gccgcgtaga ttccgtcggt tacggcgaat cccgtccggt ggccgacaac gctaccgagt
     1081 ccggtcgcgc tatcaaccgt cgcgtggaag cggaagtgga agcccaagcc cagtaatact
     1141 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1201 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1261 ttctgcgttt ata
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