BBa_K1648047 1 RBS oprF AvOprF with RBS 2015-05-30T11:00:00Z 2015-09-18T11:03:12Z / oprF with mutation K189S and all Cys to Ser. false false _2065_ 27095 13322 9 false / false Leung King Pong component2473256 1 BBa_B0034 component2473258 1 BBa_K1648045 annotation2473258 1 BBa_K1648045 range2473258 1 19 1074 annotation2473256 1 BBa_B0034 range2473256 1 1 12 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1648045 1 AvOprF AvOprF 2015-05-20T11:00:00Z 2015-09-18T01:18:43Z Synthesis. SH3 ligand interacts with SH3 domain. false false _2065_ 27095 13322 9 No part sequence false / false Leung King Pong annotation2464381 1 AvOprF range2464381 1 1 1056 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1648049 1 tetR oprF* Mutated Av oprF induced by J13002 2015-05-30T11:00:00Z 2015-09-18T06:52:02Z gBlock synthesis from IDT. oprF porin protein from Azotobacter vinelandii, with mutations K189G and all Cys to Ser. RBS (B0034) and double terminator (B0015) are included. false false _2065_ 27095 13322 9 false / false Leung King Pong component2474769 1 BBa_B0015 component2474753 1 BBa_R0040 component2474762 1 BBa_K1648047 annotation2474769 1 BBa_B0015 range2474769 1 1145 1273 annotation2474753 1 BBa_R0040 range2474753 1 1 54 annotation2474762 1 BBa_K1648047 range2474762 1 63 1136 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1648047_sequence 1 aaagaggagaaatactagatgaaactgaaaaacaccttgggcgccattattggctcgatggtcgccgcatcttcgatcagcgcgctggctcagggccagggcgcagttgaggtggaagctttcgccaagcactggttcagcgatgagagttcgcgcgacttcgaaaacgatgaagccgaactctatggtgcatcgatcggttattttttgaccgatgatgtggagctggtcttgtcgcatggcgtttacagggacgtcactaccgatgagccgattggcgacggcgggcacaaggacatcaagggtgaactgacctccatcgatgccctctatcacttcggcgagcccggcgcaggccttcgtccctacgtttccggcggcttcgcccaccagagcatcggccaggcccgtggcggccgtaacaacagcaccctggccaacgtcggcgccggtctgaagtactacttcaccgacatgctttacgcccgggccggtctcgaaggcatgtacaacttcgacgatgatcagaccgactggcaggctggcgtgggtatcggtctgaacttcggtggcagcggcgggaagcagcaacccgttgcggagccggttgccgaggtctgtgccgacgtcgacaacgatggcgtctgcgacaatgtcgaccgctgcccggatactcccgccaacaccaccgtgaatgccgagggctgcaccgccgtcgccgaggtggtgcgtgtcgagctggacgtcaagttcgacttcgacaaggcgcaggtcaaggaagaaagctatgccgacatcaagaacctggccgacttcatgaagcagtatccgcagaccaacactaccgtggaaggtcacaccgactccgtcggtacggatgcctacaaccagaagctgtccgagcgccgtgccaatgcggtgcgtgatgttctggtcaacgagtatggcgtgcaaggcagccgcgtagattccgtcggttacggcgaatcccgtccggtggccgacaacgctaccgagtccggtcgcgctatcaaccgtcgcgtggaagcggaagtggaagcccaagcccagtaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1648049_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaactgaaaaacaccttgggcgccattattggctcgatggtcgccgcatcttcgatcagcgcgctggctcagggccagggcgcagttgaggtggaagctttcgccaagcactggttcagcgatgagagttcgcgcgacttcgaaaacgatgaagccgaactctatggtgcatcgatcggttattttttgaccgatgatgtggagctggtcttgtcgcatggcgtttacagggacgtcactaccgatgagccgattggcgacggcgggcacaaggacatcaagggtgaactgacctccatcgatgccctctatcacttcggcgagcccggcgcaggccttcgtccctacgtttccggcggcttcgcccaccagagcatcggccaggcccgtggcggccgtaacaacagcaccctggccaacgtcggcgccggtctgaagtactacttcaccgacatgctttacgcccgggccggtctcgaaggcatgtacaacttcgacgatgatcagaccgactggcaggctggcgtgggtatcggtctgaacttcggtggcagcggcgggaagcagcaacccgttgcggagccggttgccgaggtctgtgccgacgtcgacaacgatggcgtctgcgacaatgtcgaccgctgcccggatactcccgccaacaccaccgtgaatgccgagggctgcaccgccgtcgccgaggtggtgcgtgtcgagctggacgtcaagttcgacttcgacaaggcgcaggtcaaggaagaaagctatgccgacatcaagaacctggccgacttcatgaagcagtatccgcagaccaacactaccgtggaaggtcacaccgactccgtcggtacggatgcctacaaccagaagctgtccgagcgccgtgccaatgcggtgcgtgatgttctggtcaacgagtatggcgtgcaaggcagccgcgtagattccgtcggttacggcgaatcccgtccggtggccgacaacgctaccgagtccggtcgcgctatcaaccgtcgcgtggaagcggaagtggaagcccaagcccagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1648045_sequence 1 atgaaactgaaaaacaccttgggcgccattattggctcgatggtcgccgcatcttcgatcagcgcgctggctcagggccagggcgcagttgaggtggaagctttcgccaagcactggttcagcgatgagagttcgcgcgacttcgaaaacgatgaagccgaactctatggtgcatcgatcggttattttttgaccgatgatgtggagctggtcttgtcgcatggcgtttacagggacgtcactaccgatgagccgattggcgacggcgggcacaaggacatcaagggtgaactgacctccatcgatgccctctatcacttcggcgagcccggcgcaggccttcgtccctacgtttccggcggcttcgcccaccagagcatcggccaggcccgtggcggccgtaacaacagcaccctggccaacgtcggcgccggtctgaagtactacttcaccgacatgctttacgcccgggccggtctcgaaggcatgtacaacttcgacgatgatcagaccgactggcaggctggcgtgggtatcggtctgaacttcggtggcagcggcgggaagcagcaacccgttgcggagccggttgccgaggtctgtgccgacgtcgacaacgatggcgtctgcgacaatgtcgaccgctgcccggatactcccgccaacaccaccgtgaatgccgagggctgcaccgccgtcgccgaggtggtgcgtgtcgagctggacgtcaagttcgacttcgacaaggcgcaggtcaaggaagaaagctatgccgacatcaagaacctggccgacttcatgaagcagtatccgcagaccaacactaccgtggaaggtcacaccgactccgtcggtacggatgcctacaaccagaagctgtccgagcgccgtgccaatgcggtgcgtgatgttctggtcaacgagtatggcgtgcaaggcagccgcgtagattccgtcggttacggcgaatcccgtccggtggccgacaacgctaccgagtccggtcgcgctatcaaccgtcgcgtggaagcggaagtggaagcccaagcccagtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z