LOCUS BBa_K1649001 4883 bp DNA linear 25-Nov-2024 DEFINITION red light sensor with a GFP reporter ACCESSION BBa_K1649001 VERSION BBa_K1649001.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..2256 /label=cph8 CDS 19..2256 /label=BBa_K592000 misc_feature 1..2256 /label=BBa_K592018 misc_feature 2269..2272 /label=conserved RBS 2265..2276 /label=BBa_B0034 misc_feature 3009..3033 /label=Help:Barcodes CDS 2283..3033 /label=BBa_I15008 misc_feature 2265..3033 /label=BBa_S03877 RBS 3042..3056 /label=RBS-1\Strong RBS 3049..3053 /label=RBS misc_feature 3042..3056 /label=BBa_B0030 RBS 3042..3056 /label=BBa_B0030 CDS 3063..3812 /label=PcyA misc_feature 3813..3837 /label=Help:Barcodes CDS 3063..3837 /label=BBa_I15009 misc_feature 3846..3884 /label=B1006 stem_loop 3855..3876 /label=modified thr terminator polyA_site 3877..3884 /label=PolyA polyA_site 3846..3854 /label=PolyA terminator 3846..3884 /label=BBa_B1006 misc_feature 3042..3884 /label=BBa_K081021 misc_feature 3905..3922 /label=C1 OmpR misc_feature 3926..3943 /label=C2 OmpR misc_feature 3946..3963 /label=C3 OmpR promoter 3967..3972 /label=-35 promoter 3990..3995 /label=-10 promoter 3893..4000 /label=BBa_R0082 misc_feature 4013..4016 /label=conserved RBS 4009..4020 /label=BBa_B0034 CDS 4027..4746 /label=GFP protein CDS 4027..4746 /label=BBa_E0040 stem_loop 4766..4809 /label=stem_loop misc_feature 4755..4834 /label=BBa_B0010 terminator 4755..4834 /label=BBa_B0010 stem_loop 4850..4869 /label=T7 TE misc_feature 4876..4876 /label=stop polyA_site 4870..4883 /label=polya misc_feature 4843..4883 /label=BBa_B0012 terminator 4843..4883 /label=BBa_B0012 misc_feature 4009..4883 /label=BBa_I13504 ORIGIN 1 aaagaggaga aatactagat ggccaccacc gtacaactca gcgaccaatc cctccgtcag 61 ctagaaaccc tcgccatcca caccgcccac ctgattcagc cccacggttt agtggtggtc 121 ctgcaagaac cagacctcac catcagccaa attagcgcca actgcaccgg cattttaggg 181 cgatcgccag aggatttgtt gggcagaacc ctaggggaag tgtttgatag ctttcagatt 241 gatcccatcc agagtcgcct aacggccgga caaatcagca gcctcaaccc cagtaaactt 301 tgggcgcggg tcatggggga cgactttgtc atttttgacg gggtttttca tcgcaacagt 361 gacggtttat tggtatgtga actcgagcca gcctacactt ccgataatct gcccttcctc 421 ggtttttatc acatggccaa cgctgccctg aatcggttgc gccaacaagc taatctacgg 481 gatttctacg atgttattgt cgaagaagtc cgccgtatga ctggctttga ccgggtgatg 541 ctataccgct ttgatgaaaa taaccacggt gatgtcattg ccgaagataa acgggatgat 601 atggaaccct atttgggcct gcactatccc gaatcggata ttccccaacc cgcccgtcgg 661 ctatttatcc acaaccccat tcgagtaatt cccgatgttt atggtgtggc ggtgcccctg 721 accccagcgg ttaaccccag caccaaccga gcggtggatt taacagaatc cattctgcgc 781 agtgcgtacc attgccactt gacctatctg aaaaatatgg gggtaggagc gtctttaacc 841 atttccctaa ttaaggacgg ccatctctgg gggctcattg cctgccacca tcaaaccccc 901 aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt ttggtcgggt ggtgtttagc 961 aacatttccg cccaggaaga tacggaaacc ttcgattacc gggtgcagct ggcggagcat 1021 gaagcggttt tattggacaa aatgaccacg gcggcggatt ttgtcgaagg attaactaat 1081 catcccgatc gcctgttggg attaacgggc tcccaggggg cggccatttg ctttggggaa 1141 aaattgattt tagtagggga aaccccggac gagaaagcag tgcaatattt actgcaatgg 1201 ttggagaatc gggaagtgca agacgttttc ttcacctctt ccctctcaca aatttatcct 1261 gatgcagtga attttaaatc cgtggccagt ggcttattgg ccattcccat tgcccgtcac 1321 aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg ttaattgggg cggtgaccca 1381 aatcatgctt acgaagctac ccaggaagac ggtaaaatcg agctccatcc ccgccaatcc 1441 tttgacctct ggaaagaaat tgtccgactc caatctttgc cctggcaatc ggtggaaatc 1501 caaagtgccc tggccctgaa aaaggcgatc gtcaacctca ttttgcgcca ggcagaagaa 1561 ttgcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg 1621 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc 1681 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc 1741 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat 1801 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa 1861 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg 1921 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctgggtcaa agtcagcagc 1981 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg 2041 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc 2101 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg 2161 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta 2221 acgcgggcgc agggcatgac aaaagaaggg taataatact agagaaagag gagaaatact 2281 agatgagtgt caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg 2341 cggagaacgt cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc 2401 gtaagctggt tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat 2461 ttaaggacca tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc 2521 tagagcaaga cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg 2581 ccgctggcca agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt 2641 tggtggccca ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga 2701 aaattgccca aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg 2761 acattgatga cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca 2821 ttgaccaagc caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca 2881 tgaaaatgtt caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca 2941 acagcctcac ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag 3001 gctaataacg ctgatagtgc tagtgtagat cgctactaga gattaaagag gagaaatact 3061 agatggccgt cactgattta agtttgacca attcttccct gatgcctacg ttgaacccga 3121 tgattcaaca gttggccctg gcgatcgccg ctagttggca aagtttaccc ctcaagccct 3181 atcaattgcc ggaggatttg ggctacgtag aaggccgcct ggaaggggaa aagttagtga 3241 ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat gcatttggag ttggccaagg 3301 tgggcaaagg gttggatatt ctccactgtg taatgtttcc tgagccttta tacggtctac 3361 ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt aagtgcggct attgcggatc 3421 tatcccccac ccaaagcgat cgccaattgc ccgcagcgta ccaaaaatca ttggcagagc 3481 taggccagcc agaatttgag caacaacggg aattgccccc ctggggagaa atattttctg 3541 aatattgttt attcatccgt cccagcaatg tcactgaaga agaaagattt gtacaaaggg 3601 tagtggactt tttgcaaatt cattgtcacc aatccatcgt tgccgaaccc ttgtctgaag 3661 ctcaaacttt ggagcaccgt caggggcaaa ttcattactg ccaacaacaa cagaaaaatg 3721 ataaaacccg tcgggtactg gaaaaagctt ttggggaagc ttgggcggaa cggtatatga 3781 gccaagtctt atttgatgtt atccaataat aactctgata gtgctagtgt agatctctac 3841 tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttttactag agtcccttgc 3901 atttacattt tgaaacatct atagcgataa atgaaacatc ttaaaagttt tagtatcata 3961 ttcgtgttgg attattctgc atttttgggg agaatggact tactagagaa agaggagaaa 4021 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 4081 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 4141 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 4201 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg 4261 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 4321 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 4381 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 4441 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 4501 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 4561 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 4621 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 4681 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 4741 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 4801 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 4861 gggtgggcct ttctgcgttt ata //