##gff-version 3
##sequence-region BBa_K1649001 1 4883
BBa_K1649001	.	engineered_region	1	2256	.	+	0	ID=BBa_K592018;Name=BBa_K592018
BBa_K1649001	.	CDS	19	2256	.	+	0	ID=BBa_K592000;Name=BBa_K592000;Parent=BBa_K592018
BBa_K1649001	.	CDS	19	2256	.	+	0	ID=annotation2132074;Name=cph8;Parent=BBa_K592000
BBa_K1649001	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592018
BBa_K1649001	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649001	.	engineered_region	3042	3884	.	+	0	ID=BBa_K081021;Name=BBa_K081021
BBa_K1649001	.	CDS	3063	3837	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081021
BBa_K1649001	.	engineered_tag	3813	3837	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K1649001	.	CDS	3063	3812	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K1649001	.	terminator	3846	3884	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081021
BBa_K1649001	.	polyA_site	3846	3854	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1649001	.	stem_loop	3855	3876	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1649001	.	polyA_site	3877	3884	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1649001	.	engineered_region	3846	3884	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1649001	.	ribosome_entry_site	3042	3056	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081021
BBa_K1649001	.	engineered_region	3042	3056	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1649001	.	ribosome_entry_site	3049	3053	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1649001	.	ribosome_entry_site	3042	3056	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1649001	.	engineered_region	4009	4883	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_K1649001	.	ribosome_entry_site	4009	4020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_K1649001	.	conserved_region	4013	4016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649001	.	terminator	4843	4883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_K1649001	.	stem_loop	4850	4869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1649001	.	stop_codon	4876	4876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1649001	.	engineered_region	4843	4883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1649001	.	polyA_site	4870	4883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1649001	.	CDS	4027	4746	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_K1649001	.	CDS	4027	4746	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1649001	.	terminator	4755	4834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_K1649001	.	stem_loop	4766	4809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1649001	.	engineered_region	4755	4834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1649001	.	engineered_region	2265	3033	.	+	0	ID=BBa_S03877;Name=BBa_S03877
BBa_K1649001	.	CDS	2283	3033	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_S03877
BBa_K1649001	.	engineered_tag	3009	3033	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_K1649001	.	ribosome_entry_site	2265	2276	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03877
BBa_K1649001	.	conserved_region	2269	2272	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649001	.	promoter	3893	4000	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K1649001	.	non_covalent_binding_site	3946	3963	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1649001	.	promoter	3990	3995	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1649001	.	non_covalent_binding_site	3926	3943	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1649001	.	non_covalent_binding_site	3905	3922	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1649001	.	promoter	3967	3972	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
>BBa_K1649001
aaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatcc
acaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgc
caactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcag
attgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgg
gggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagccta
cacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaat
ctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttg
atgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccga
atcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcg
gtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtacc
attgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctg
ggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgg
gtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcgg
ttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaac
gggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaa
tatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatg
cagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccc
tgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgag
ctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatcc
aaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgt
taagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgc
ctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgcca
tcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcgg
tgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatg
cacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtca
gcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagca
cctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgt
atcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccag
tgccggtaacgcgggcgcagggcatgacaaaagaagggtaataatactagagaaagaggagaaatactagatgagtgtc
aacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcc
tcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagagga
aatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaa
gacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgag
tccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcgg
tcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacatt
gatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacgga
ttgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgat
cggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggc
taataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaa
gtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggca
aagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtg
attgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggata
ttctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtgg
agtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggca
gagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattca
tccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatc
catcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacag
aaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttat
ttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacaggg
cggggtttttttttactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttt
tagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatg
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatttt
ctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacc
tgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacag
catgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactaca
agacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaga
tggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaat
ggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaata
ctccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaa
cgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata