LOCUS BBa_K1649002 7280 bp DNA linear 25-Nov-2024 DEFINITION Red light sensor with TetR not gate and GFP reporter ACCESSION BBa_K1649002 VERSION BBa_K1649002.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..2256 /label=cph8 CDS 19..2256 /label=BBa_K592000 misc_feature 1..2256 /label=BBa_K592018 misc_feature 2269..2272 /label=conserved RBS 2265..2276 /label=BBa_B0034 CDS 2283..4520 /label=cph8 CDS 2283..4520 /label=BBa_K592000 misc_feature 2265..4520 /label=BBa_K592018 RBS 4529..4543 /label=RBS-1\Strong RBS 4536..4540 /label=RBS misc_feature 4529..4543 /label=BBa_B0030 RBS 4529..4543 /label=BBa_B0030 CDS 4550..5299 /label=PcyA misc_feature 5300..5324 /label=Help:Barcodes CDS 4550..5324 /label=BBa_I15009 misc_feature 5333..5371 /label=B1006 stem_loop 5342..5363 /label=modified thr terminator polyA_site 5364..5371 /label=PolyA polyA_site 5333..5341 /label=PolyA terminator 5333..5371 /label=BBa_B1006 misc_feature 4529..5371 /label=BBa_K081021 misc_feature 5392..5409 /label=C1 OmpR misc_feature 5413..5430 /label=C2 OmpR misc_feature 5433..5450 /label=C3 OmpR promoter 5454..5459 /label=-35 promoter 5477..5482 /label=-10 promoter 5380..5487 /label=BBa_R0082 misc_feature 5500..5503 /label=conserved RBS 5496..5507 /label=BBa_B0034 CDS 5517..6133 /label=tetR misc_feature 6134..6167 /label=SsrA misc_feature 6174..6198 /label=Help:Barcodes CDS 5514..6198 /label=BBa_C0040 misc_feature 5496..6198 /label=BBa_S03518 stem_loop 6218..6261 /label=stem_loop misc_feature 6207..6286 /label=BBa_B0010 terminator 6207..6286 /label=BBa_B0010 stem_loop 6302..6321 /label=T7 TE misc_feature 6328..6328 /label=stop polyA_site 6322..6335 /label=polya misc_feature 6295..6335 /label=BBa_B0012 terminator 6295..6335 /label=BBa_B0012 terminator 6207..6335 /label=BBa_B0015 misc_feature 6344..6362 /label=TetR 1 misc_feature 6344..6397 /label=BBa_R0040 promoter 6363..6368 /label=-35 misc_feature 6369..6387 /label=TetR 2 promoter 6386..6391 /label=-10 promoter 6344..6397 /label=BBa_R0040 misc_feature 6410..6413 /label=conserved RBS 6406..6417 /label=BBa_B0034 CDS 6424..7143 /label=GFP protein CDS 6424..7143 /label=BBa_E0040 stem_loop 7163..7206 /label=stem_loop misc_feature 7152..7231 /label=BBa_B0010 terminator 7152..7231 /label=BBa_B0010 stem_loop 7247..7266 /label=T7 TE misc_feature 7273..7273 /label=stop polyA_site 7267..7280 /label=polya misc_feature 7240..7280 /label=BBa_B0012 terminator 7240..7280 /label=BBa_B0012 misc_feature 6406..7280 /label=BBa_I13504 ORIGIN 1 aaagaggaga aatactagat ggccaccacc gtacaactca gcgaccaatc cctccgtcag 61 ctagaaaccc tcgccatcca caccgcccac ctgattcagc cccacggttt agtggtggtc 121 ctgcaagaac cagacctcac catcagccaa attagcgcca actgcaccgg cattttaggg 181 cgatcgccag aggatttgtt gggcagaacc ctaggggaag tgtttgatag ctttcagatt 241 gatcccatcc agagtcgcct aacggccgga caaatcagca gcctcaaccc cagtaaactt 301 tgggcgcggg tcatggggga cgactttgtc atttttgacg gggtttttca tcgcaacagt 361 gacggtttat tggtatgtga actcgagcca gcctacactt ccgataatct gcccttcctc 421 ggtttttatc acatggccaa cgctgccctg aatcggttgc gccaacaagc taatctacgg 481 gatttctacg atgttattgt cgaagaagtc cgccgtatga ctggctttga ccgggtgatg 541 ctataccgct ttgatgaaaa taaccacggt gatgtcattg ccgaagataa acgggatgat 601 atggaaccct atttgggcct gcactatccc gaatcggata ttccccaacc cgcccgtcgg 661 ctatttatcc acaaccccat tcgagtaatt cccgatgttt atggtgtggc ggtgcccctg 721 accccagcgg ttaaccccag caccaaccga gcggtggatt taacagaatc cattctgcgc 781 agtgcgtacc attgccactt gacctatctg aaaaatatgg gggtaggagc gtctttaacc 841 atttccctaa ttaaggacgg ccatctctgg gggctcattg cctgccacca tcaaaccccc 901 aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt ttggtcgggt ggtgtttagc 961 aacatttccg cccaggaaga tacggaaacc ttcgattacc gggtgcagct ggcggagcat 1021 gaagcggttt tattggacaa aatgaccacg gcggcggatt ttgtcgaagg attaactaat 1081 catcccgatc gcctgttggg attaacgggc tcccaggggg cggccatttg ctttggggaa 1141 aaattgattt tagtagggga aaccccggac gagaaagcag tgcaatattt actgcaatgg 1201 ttggagaatc gggaagtgca agacgttttc ttcacctctt ccctctcaca aatttatcct 1261 gatgcagtga attttaaatc cgtggccagt ggcttattgg ccattcccat tgcccgtcac 1321 aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg ttaattgggg cggtgaccca 1381 aatcatgctt acgaagctac ccaggaagac ggtaaaatcg agctccatcc ccgccaatcc 1441 tttgacctct ggaaagaaat tgtccgactc caatctttgc cctggcaatc ggtggaaatc 1501 caaagtgccc tggccctgaa aaaggcgatc gtcaacctca ttttgcgcca ggcagaagaa 1561 ttgcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg 1621 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc 1681 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc 1741 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat 1801 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa 1861 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg 1921 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctgggtcaa agtcagcagc 1981 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg 2041 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc 2101 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg 2161 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta 2221 acgcgggcgc agggcatgac aaaagaaggg taataatact agagaaagag gagaaatact 2281 agatggccac caccgtacaa ctcagcgacc aatccctccg tcagctagaa accctcgcca 2341 tccacaccgc ccacctgatt cagccccacg gtttagtggt ggtcctgcaa gaaccagacc 2401 tcaccatcag ccaaattagc gccaactgca ccggcatttt agggcgatcg ccagaggatt 2461 tgttgggcag aaccctaggg gaagtgtttg atagctttca gattgatccc atccagagtc 2521 gcctaacggc cggacaaatc agcagcctca accccagtaa actttgggcg cgggtcatgg 2581 gggacgactt tgtcattttt gacggggttt ttcatcgcaa cagtgacggt ttattggtat 2641 gtgaactcga gccagcctac acttccgata atctgccctt cctcggtttt tatcacatgg 2701 ccaacgctgc cctgaatcgg ttgcgccaac aagctaatct acgggatttc tacgatgtta 2761 ttgtcgaaga agtccgccgt atgactggct ttgaccgggt gatgctatac cgctttgatg 2821 aaaataacca cggtgatgtc attgccgaag ataaacggga tgatatggaa ccctatttgg 2881 gcctgcacta tcccgaatcg gatattcccc aacccgcccg tcggctattt atccacaacc 2941 ccattcgagt aattcccgat gtttatggtg tggcggtgcc cctgacccca gcggttaacc 3001 ccagcaccaa ccgagcggtg gatttaacag aatccattct gcgcagtgcg taccattgcc 3061 acttgaccta tctgaaaaat atgggggtag gagcgtcttt aaccatttcc ctaattaagg 3121 acggccatct ctgggggctc attgcctgcc accatcaaac ccccaaagta attccctttg 3181 aactgcgtaa agcctgcgaa ttttttggtc gggtggtgtt tagcaacatt tccgcccagg 3241 aagatacgga aaccttcgat taccgggtgc agctggcgga gcatgaagcg gttttattgg 3301 acaaaatgac cacggcggcg gattttgtcg aaggattaac taatcatccc gatcgcctgt 3361 tgggattaac gggctcccag ggggcggcca tttgctttgg ggaaaaattg attttagtag 3421 gggaaacccc ggacgagaaa gcagtgcaat atttactgca atggttggag aatcgggaag 3481 tgcaagacgt tttcttcacc tcttccctct cacaaattta tcctgatgca gtgaatttta 3541 aatccgtggc cagtggctta ttggccattc ccattgcccg tcacaacttt ttgctctggt 3601 ttcgccctga agtgttgcaa acggttaatt ggggcggtga cccaaatcat gcttacgaag 3661 ctacccagga agacggtaaa atcgagctcc atccccgcca atcctttgac ctctggaaag 3721 aaattgtccg actccaatct ttgccctggc aatcggtgga aatccaaagt gccctggccc 3781 tgaaaaaggc gatcgtcaac ctcattttgc gccaggcaga agaattgcat atggcggctg 3841 gtgttaagca actggcggat gaccgcacgc tgctgatggc gggggtaagt cacgacttgc 3901 gcacgccgct gacgcgtatt cgcctggcga ctgagatgat gagcgagcag gatggctatc 3961 tggcagaatc gatcaataaa gatatcgaag agtgcaacgc catcattgag cagtttatcg 4021 actacctgcg caccgggcag gagatgccga tggaaatggc ggatcttaat gcagtactcg 4081 gtgaggtgat tgctgccgaa agtggctatg agcgggaaat tgaaaccgcg ctttaccccg 4141 gcagcattga agtgaaaatg cacccgctgt cgatcaaacg cgcggtggcg aatatggtgg 4201 tcaacgccgc ccgttatggc aatggctggg tcaaagtcag cagcggaacg gagccgaatc 4261 gcgcctggtt ccaggtggaa gatgacggtc cgggaattgc gccggaacaa cgtaagcacc 4321 tgttccagcc gtttgtccgc ggcgacagtg cgcgcaccat tagcggcacg ggattagggc 4381 tggcaattgt gcagcgtatc gtggataacc ataacgggat gctggagctt ggcaccagcg 4441 agcggggcgg gctttccatt cgcgcctggc tgccagtgcc ggtaacgcgg gcgcagggca 4501 tgacaaaaga agggtaataa tactagagat taaagaggag aaatactaga tggccgtcac 4561 tgatttaagt ttgaccaatt cttccctgat gcctacgttg aacccgatga ttcaacagtt 4621 ggccctggcg atcgccgcta gttggcaaag tttacccctc aagccctatc aattgccgga 4681 ggatttgggc tacgtagaag gccgcctgga aggggaaaag ttagtgattg aaaatcggtg 4741 ctaccaaacg ccccagtttc gcaaaatgca tttggagttg gccaaggtgg gcaaagggtt 4801 ggatattctc cactgtgtaa tgtttcctga gcctttatac ggtctacctt tgtttggctg 4861 tgacattgtg gccggccccg gtggagtaag tgcggctatt gcggatctat cccccaccca 4921 aagcgatcgc caattgcccg cagcgtacca aaaatcattg gcagagctag gccagccaga 4981 atttgagcaa caacgggaat tgcccccctg gggagaaata ttttctgaat attgtttatt 5041 catccgtccc agcaatgtca ctgaagaaga aagatttgta caaagggtag tggacttttt 5101 gcaaattcat tgtcaccaat ccatcgttgc cgaacccttg tctgaagctc aaactttgga 5161 gcaccgtcag gggcaaattc attactgcca acaacaacag aaaaatgata aaacccgtcg 5221 ggtactggaa aaagcttttg gggaagcttg ggcggaacgg tatatgagcc aagtcttatt 5281 tgatgttatc caataataac tctgatagtg ctagtgtaga tctctactag agaaaaaaaa 5341 accccgcccc tgacagggcg gggttttttt ttactagagt cccttgcatt tacattttga 5401 aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt 5461 attctgcatt tttggggaga atggacttac tagagaaaga ggagaaatac tagatgtcca 5521 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 5581 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 5641 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 5701 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 5761 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 5821 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 5881 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 5941 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 6001 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 6061 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 6121 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 6181 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 6241 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 6301 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga 6361 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac 6421 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 6481 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 6541 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 6601 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa 6661 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 6721 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 6781 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 6841 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat 6901 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca 6961 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 7021 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc 7081 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 7141 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 7201 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 7261 tgggcctttc tgcgtttata //