##gff-version 3
##sequence-region BBa_K1649002 1 7280
BBa_K1649002	.	engineered_region	2265	4520	.	+	0	ID=BBa_K592018;Name=BBa_K592018
BBa_K1649002	.	CDS	2283	4520	.	+	0	ID=BBa_K592000;Name=BBa_K592000;Parent=BBa_K592018
BBa_K1649002	.	CDS	2283	4520	.	+	0	ID=annotation2132074;Name=cph8;Parent=BBa_K592000
BBa_K1649002	.	ribosome_entry_site	2265	2276	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592018
BBa_K1649002	.	conserved_region	2269	2272	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649002	.	engineered_region	4529	5371	.	+	0	ID=BBa_K081021;Name=BBa_K081021
BBa_K1649002	.	ribosome_entry_site	4529	4543	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081021
BBa_K1649002	.	ribosome_entry_site	4529	4543	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1649002	.	ribosome_entry_site	4536	4540	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1649002	.	engineered_region	4529	4543	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1649002	.	terminator	5333	5371	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081021
BBa_K1649002	.	stem_loop	5342	5363	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1649002	.	polyA_site	5333	5341	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1649002	.	polyA_site	5364	5371	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1649002	.	engineered_region	5333	5371	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1649002	.	CDS	4550	5324	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081021
BBa_K1649002	.	CDS	4550	5299	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K1649002	.	engineered_tag	5300	5324	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K1649002	.	engineered_region	1	2256	.	+	0	ID=BBa_K592018;Name=BBa_K592018
BBa_K1649002	.	CDS	19	2256	.	+	0	ID=BBa_K592000;Name=BBa_K592000;Parent=BBa_K592018
BBa_K1649002	.	CDS	19	2256	.	+	0	ID=annotation2132074;Name=cph8;Parent=BBa_K592000
BBa_K1649002	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592018
BBa_K1649002	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649002	.	promoter	6344	6397	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1649002	.	promoter	6363	6368	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1649002	.	promoter	6386	6391	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1649002	.	non_covalent_binding_site	6344	6362	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1649002	.	engineered_region	6344	6397	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1649002	.	non_covalent_binding_site	6369	6387	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1649002	.	engineered_region	6406	7280	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_K1649002	.	ribosome_entry_site	6406	6417	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_K1649002	.	conserved_region	6410	6413	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649002	.	terminator	7240	7280	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_K1649002	.	polyA_site	7267	7280	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1649002	.	stem_loop	7247	7266	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1649002	.	engineered_region	7240	7280	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1649002	.	stop_codon	7273	7273	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1649002	.	terminator	7152	7231	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_K1649002	.	stem_loop	7163	7206	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1649002	.	engineered_region	7152	7231	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1649002	.	CDS	6424	7143	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_K1649002	.	CDS	6424	7143	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1649002	.	engineered_region	5496	6198	.	+	0	ID=BBa_S03518;Name=BBa_S03518
BBa_K1649002	.	ribosome_entry_site	5496	5507	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_K1649002	.	conserved_region	5500	5503	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649002	.	CDS	5514	6198	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_K1649002	.	CDS	5517	6133	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1649002	.	tag	6134	6167	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1649002	.	engineered_tag	6174	6198	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1649002	.	promoter	5380	5487	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K1649002	.	non_covalent_binding_site	5392	5409	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1649002	.	non_covalent_binding_site	5413	5430	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1649002	.	promoter	5477	5482	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1649002	.	non_covalent_binding_site	5433	5450	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1649002	.	promoter	5454	5459	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1649002	.	terminator	6207	6335	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1649002	.	terminator	6207	6286	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1649002	.	stem_loop	6218	6261	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1649002	.	engineered_region	6207	6286	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1649002	.	terminator	6295	6335	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1649002	.	polyA_site	6322	6335	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1649002	.	stem_loop	6302	6321	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1649002	.	engineered_region	6295	6335	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1649002	.	stop_codon	6328	6328	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1649002
aaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatcc
acaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgc
caactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcag
attgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgg
gggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagccta
cacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaat
ctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttg
atgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccga
atcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcg
gtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtacc
attgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctg
ggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgg
gtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcgg
ttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaac
gggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaa
tatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatg
cagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccc
tgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgag
ctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatcc
aaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgt
taagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgc
ctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgcca
tcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcgg
tgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatg
cacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtca
gcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagca
cctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgt
atcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccag
tgccggtaacgcgggcgcagggcatgacaaaagaagggtaataatactagagaaagaggagaaatactagatggccacc
accgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacg
gtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatc
gccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacg
gccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacgggg
tttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcgg
tttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtc
gaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattg
ccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcg
gctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaacccc
agcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaata
tgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaac
ccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccag
gaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcgg
cggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctt
tggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgg
gaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtg
gcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattgggg
cggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctc
tggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcga
tcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgct
gctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcag
gatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgc
gcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggcta
tgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtg
gcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcct
ggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcga
cagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctg
gagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatga
caaaagaagggtaataatactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattct
tccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctca
agccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtg
ctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgta
atgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggcta
ttgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagcc
agaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaat
gtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaac
ccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaac
ccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaa
taataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggttttttttt
actagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcg
tgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacta
ctagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata