LOCUS BBa_K1649003 3117 bp DNA linear 22-Dec-2024 DEFINITION YF1 blue light sensor with GFP reporter ACCESSION BBa_K1649003 VERSION BBa_K1649003.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23102 misc_feature 1..6 /label=-35 box misc_feature 24..29 /label=-10 box promoter 1..35 /label=BBa_K823006 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1192 /label=YF1 CDS 62..1192 /label=BBa_K592004 misc_feature 1205..1208 /label=conserved RBS 1201..1212 /label=BBa_B0034 CDS 1219..1839 /label=FixJ CDS 1219..1839 /label=BBa_K592005 misc_feature 44..1839 /label=BBa_K592016 stem_loop 1859..1902 /label=stem_loop misc_feature 1848..1927 /label=BBa_B0010 terminator 1848..1927 /label=BBa_B0010 stem_loop 1943..1962 /label=T7 TE misc_feature 1969..1969 /label=stop polyA_site 1963..1976 /label=polya misc_feature 1936..1976 /label=BBa_B0012 terminator 1936..1976 /label=BBa_B0012 terminator 1848..1976 /label=BBa_B0015 promoter 1985..2234 /label=FixK2 promoter promoter 1985..2234 /label=BBa_K592006 misc_feature 2247..2250 /label=conserved RBS 2243..2254 /label=BBa_B0034 CDS 2261..2980 /label=GFP protein CDS 2261..2980 /label=BBa_E0040 stem_loop 3000..3043 /label=stem_loop misc_feature 2989..3068 /label=BBa_B0010 terminator 2989..3068 /label=BBa_B0010 stem_loop 3084..3103 /label=T7 TE misc_feature 3110..3110 /label=stop polyA_site 3104..3117 /label=polya misc_feature 3077..3117 /label=BBa_B0012 terminator 3077..3117 /label=BBa_B0012 misc_feature 2243..3117 /label=BBa_I13504 ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta 61 gatggctagt tttcaatcat ttgggatacc aggacagctg gaagtcatca aaaaagcact 121 tgatcacgtg cgagtcggtg tggtaattac agatcccgca cttgaagata atcctattgt 181 ctacgtaaat caaggctttg ttcaaatgac cggctacgag accgaggaaa ttttaggaaa 241 gaactgtcgc ttcttacagg ggaaacacac agatccggca gaagtggaca acatcagaac 301 cgctttacaa aataaagaac cggtcaccgt tcagatccaa aactacaaaa aagacggaac 361 gatgttctgg aatgaattaa atattgatcc aatggaaata gaggataaaa cgtattttgt 421 cggcattcag aatgatatca ccgagcacca gcagacccag gcgcgtctcc aggaactgca 481 atccgagctc gtccacgtct ccaggctgag cgccatgggc gaaatggcgt ccgcgctcgc 541 gcacgagctc aaccagccgc tggcggcgat cagcaactac atgaagggct cgcggcggct 601 gcttgccggc agcagtgatc cgaacacacc gaaggtcgaa agcgccctgg accgcgccgc 661 cgagcaggcg ctgcgcgccg gccagatcat ccggcgcctg cgcgacttcg ttgcccgcgg 721 cgaatcggag aagcgggtcg agagtctctc caagctgatc gaggaggccg gcgcgctcgg 781 gcttgccggc gcgcgcgagc agaacgtgca gctccgcttc agtctcgatc cgggcgccga 841 tctcgttctc gccgaccggg tgcagatcca gcaggtcctg gtcaacctgt tccgcaacgc 901 gctggaagcg atggctcagt cgcagcgacg cgagctcgtc gtcaccaaca cccccgccgc 961 cgacgacatg atcgaggtcg aagtgtccga caccggcagc ggtttccagg acgacgtcat 1021 tccgaacctg tttcagactt tcttcaccac caaggacacc ggcatgggcg tgggactgtc 1081 catcagccgc tcgatcatcg aagctcacgg cgggcgcatg tgggccgaga gcaacgcatc 1141 gggcggggcg accttccgct tcaccctccc ggcagccgac gagaattaat aatactagag 1201 aaagaggaga aatactagat gacgaccaag ggacatatct acgtcatcga cgacgacgcg 1261 gcgatgcggg attcgctgaa tttcctgctg gattctgccg gcttcggcgt cacgctgttt 1321 gacgacgcgc aagcctttct cgacgccctg ccgggtctct ccttcggctg cgtcgtctcc 1381 gacgtgcgca tgccgggcct tgacggcatc gagctgttga agcggatgaa ggcgcagcaa 1441 agcccctttc cgatcctcat catgaccggt cacggcgacg tgccgctcgc ggtcgaggcg 1501 atgaagttag gggcggtcga ctttctggaa aagcctttcg aggacgaccg cctcaccgcc 1561 atgatcgaat cggcgatccg ccaggccgag ccggccgcca agagcgaggc cgtcgcgcag 1621 gatatcgccg cccgcgtcgc ctcgttgagc cccagggagc gccaggtcat ggaagggctg 1681 atcgccggcc tttccaacaa gctgatcgcc cgcgagtacg acatcagccc gcgcaccatc 1741 gaggtgtatc gggccaacgt catgaccaag atgcaggcca acagcctttc ggagctggtt 1801 cgcctcgcga tgcgcgccgg catgctcaac gattaataat actagagcca ggcatcaaat 1861 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1921 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 1981 agaggccgga gccgattatc cgcacccgtc cttggtcttg gacacactcg ctcgcgatcc 2041 gaagccgcct ttcaaagata ctccgcagtg aaacgcatct tttaagcgcg acttgtcgcc 2101 ttcgcgcagc agaacactcg tatggcactc aatccatgat cgcttcgttc gctccgacgc 2161 gcgttgcggg gcccccctcg accggatgac aacacattgc gtcacctcaa ctgctgcgcg 2221 cgcactgcgt cacatactag agaaagagga gaaatactag atgcgtaaag gagaagaact 2281 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt 2341 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat 2401 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg 2461 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc 2521 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa 2581 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg 2641 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc 2701 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat 2761 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc 2821 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct 2881 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc 2941 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa 3001 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3061 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //