##gff-version 3
##sequence-region BBa_K1649003 1 3117
BBa_K1649003	.	engineered_region	44	1839	.	+	0	ID=BBa_K592016;Name=BBa_K592016
BBa_K1649003	.	CDS	62	1192	.	+	0	ID=BBa_K592004;Name=BBa_K592004;Parent=BBa_K592016
BBa_K1649003	.	CDS	62	1192	.	+	0	ID=annotation2130111;Name=YF1;Parent=BBa_K592004
BBa_K1649003	.	ribosome_entry_site	1201	1212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1649003	.	conserved_region	1205	1208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649003	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1649003	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649003	.	CDS	1219	1839	.	+	0	ID=BBa_K592005;Name=BBa_K592005;Parent=BBa_K592016
BBa_K1649003	.	CDS	1219	1839	.	+	0	ID=annotation2130114;Name=FixJ;Parent=BBa_K592005
BBa_K1649003	.	promoter	1985	2234	.	+	0	ID=BBa_K592006;Name=BBa_K592006
BBa_K1649003	.	promoter	1985	2234	.	+	0	ID=annotation2130179;Name=FixK2 promoter;Parent=BBa_K592006
BBa_K1649003	.	promoter	1	35	.	+	0	ID=BBa_K823006;Name=BBa_K823006
BBa_K1649003	.	promoter	1	35	.	+	0	ID=annotation2182512;Name=J23102;Parent=BBa_K823006
BBa_K1649003	.	non_covalent_binding_site	24	29	.	+	0	ID=annotation2182514;Name=-10 box;Parent=BBa_K823006
BBa_K1649003	.	non_covalent_binding_site	1	6	.	+	0	ID=annotation2182513;Name=-35 box;Parent=BBa_K823006
BBa_K1649003	.	terminator	1848	1976	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1649003	.	terminator	1848	1927	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1649003	.	engineered_region	1848	1927	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1649003	.	stem_loop	1859	1902	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1649003	.	terminator	1936	1976	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1649003	.	engineered_region	1936	1976	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1649003	.	stop_codon	1969	1969	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1649003	.	polyA_site	1963	1976	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1649003	.	stem_loop	1943	1962	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1649003	.	engineered_region	2243	3117	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_K1649003	.	CDS	2261	2980	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_K1649003	.	CDS	2261	2980	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1649003	.	ribosome_entry_site	2243	2254	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_K1649003	.	conserved_region	2247	2250	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1649003	.	terminator	3077	3117	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_K1649003	.	engineered_region	3077	3117	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1649003	.	stop_codon	3110	3110	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1649003	.	polyA_site	3104	3117	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1649003	.	stem_loop	3084	3103	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1649003	.	terminator	2989	3068	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_K1649003	.	engineered_region	2989	3068	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1649003	.	stem_loop	3000	3043	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1649003
ttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatggctagttttcaatca
tttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccg
cacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttagg
aaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaa
gaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatgg
aaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccagga
actgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaac
cagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccga
aggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgt
tgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggc
gcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatcc
agcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaa
cacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaac
ctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctc
acggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaa
ttaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcga
tgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcga
cgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaag
cggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcga
tgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccg
ccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggag
cgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcacca
tcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgc
cggcatgctcaacgattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagaggccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttca
aagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaat
ccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaact
gctgcgcgcgcactgcgtcacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata