BBa_K1649003
type: ComponentDefinition
persistent identity: BBa K1649003
display id: BBa_K1649003
version: 1
was generated by: iGEM to SBOL conversion
description: YF1 blue light sensor with GFP reporter
created: 2015-09-06T11:00:00Z
modified: 2015-09-18T07:51:17Z
mutable provenance: kit plate
owned by: james
owned by: myers
mutable description: GFP is used to report the function of blue light sensor. The LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2066_
m user id: 24386
owner id: 24386
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: To test the funnction blue light sensor
star: false
creator: Yang Yang
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823006
component: BBa_B0015
component: BBa_K592016
component: BBa_I13504
component: BBa_K592006
sequence annotation: BBa_K823006
sequence annotation: BBa_K592016
sequence annotation: BBa_B0015
sequence annotation: BBa_K592006
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K1649003_sequence/1

BBa_K823006
type: Component
persistent identity: component2445533
display id: component2445533
version: 1
top level: BBa_K1649003
definition: BBa_K823006
access: public

BBa_B0015
type: Component
persistent identity: component2445549
display id: component2445549
version: 1
top level: BBa_K1649003
definition: BBa_B0015
access: public

BBa_K592016
type: Component
persistent identity: component2445542
display id: component2445542
version: 1
top level: BBa_K1649003
definition: BBa_K592016
access: public

BBa_I13504
type: Component
persistent identity: component2445561
display id: component2445561
version: 1
top level: BBa_K1649003
definition: BBa_I13504
access: public

BBa_K592006
type: Component
persistent identity: component2445551
display id: component2445551
version: 1
top level: BBa_K1649003
definition: BBa_K592006
access: public

BBa_K823006
type: SequenceAnnotation
persistent identity: annotation2445533
display id: annotation2445533
version: 1
top level: BBa_K1649003
location: https://synbiohub.org/public/igem/BBa_K1649003/annotation2445533/range2445533/1
component: BBa_K823006

https://synbiohub.org/public/igem/BBa_K1649003/annotation2445533/range2445533/1
type: Range
persistent identity: range2445533
display id: range2445533
version: 1
top level: BBa_K1649003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2445542
display id: annotation2445542
version: 1
top level: BBa_K1649003
location: https://synbiohub.org/public/igem/BBa_K1649003/annotation2445542/range2445542/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K1649003/annotation2445542/range2445542/1
type: Range
persistent identity: range2445542
display id: range2445542
version: 1
top level: BBa_K1649003
direction: direction/reverse
start: 44
end: 1839
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2445549
display id: annotation2445549
version: 1
top level: BBa_K1649003
location: https://synbiohub.org/public/igem/BBa_K1649003/annotation2445549/range2445549/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1649003/annotation2445549/range2445549/1
type: Range
persistent identity: range2445549
display id: range2445549
version: 1
top level: BBa_K1649003
direction: direction/reverse
start: 1848
end: 1976
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2445551
display id: annotation2445551
version: 1
top level: BBa_K1649003
location: https://synbiohub.org/public/igem/BBa_K1649003/annotation2445551/range2445551/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K1649003/annotation2445551/range2445551/1
type: Range
persistent identity: range2445551
display id: range2445551
version: 1
top level: BBa_K1649003
direction: direction/reverse
start: 1985
end: 2234
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2445561
display id: annotation2445561
version: 1
top level: BBa_K1649003
location: https://synbiohub.org/public/igem/BBa_K1649003/annotation2445561/range2445561/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1649003/annotation2445561/range2445561/1
type: Range
persistent identity: range2445561
display id: range2445561
version: 1
top level: BBa_K1649003
direction: direction/reverse
start: 2243
end: 3117
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
top level: BBa_I13504
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
top level: BBa_I13504
direction: direction/forward
start: 19
end: 738
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
top level: BBa_I13504
direction: direction/forward
start: 747
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
top level: BBa_I13504
direction: direction/forward
start: 835
end: 875
orientation: inline

BBa_K592006
type: ComponentDefinition
persistent identity: BBa K592006
display id: BBa_K592006
version: 1
was generated by: iGEM to SBOL conversion
description: FixK2 promoter
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: FixK2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592006_sequence/1

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2130179
display id: annotation2130179
version: 1
top level: BBa_K592006
location: https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
type: Range
persistent identity: range2130179
display id: range2130179
version: 1
top level: BBa_K592006
direction: direction/forward
start: 1
end: 250
orientation: inline

BBa_K592016
type: ComponentDefinition
persistent identity: BBa K592016
display id: BBa_K592016
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This constructs Strong RBS to ensure good expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592004
component: BBa_B0034
component: BBa_K592005
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence annotation: BBa_K592005
sequence: https://synbiohub.org/public/igem/BBa_K592016_sequence/1

BBa_K592004
type: Component
persistent identity: component2136335
display id: component2136335
version: 1
top level: BBa_K592016
definition: YF1
access: public

BBa_B0034
type: Component
persistent identity: component2136333
display id: component2136333
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_K592005
type: Component
persistent identity: component2136339
display id: component2136339
version: 1
top level: BBa_K592016
definition: FixJ
access: public

BBa_B0034
type: Component
persistent identity: component2136337
display id: component2136337
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136333
display id: annotation2136333
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
type: Range
persistent identity: range2136333
display id: range2136333
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136335
display id: annotation2136335
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
type: Range
persistent identity: range2136335
display id: range2136335
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136337
display id: annotation2136337
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
type: Range
persistent identity: range2136337
display id: range2136337
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2136339
display id: annotation2136339
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
component: BBa_K592005

https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
type: Range
persistent identity: range2136339
display id: range2136339
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1176
end: 1796
orientation: inline

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson promoter J23102
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
mutable provenance: Partsregistry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23102
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
top level: BBa_K823006
direction: direction/forward
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
top level: BBa_K823006
direction: direction/forward
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
top level: BBa_K823006
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

YF1
type: ComponentDefinition
persistent identity: BBa K592004
display id: BBa_K592004
version: 1
was generated by: iGEM to SBOL conversion
description: YF1 blue-light sensor protein
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: YF1
sequence: https://synbiohub.org/public/igem/BBa_K592004_sequence/1

YF1
type: SequenceAnnotation
persistent identity: annotation2130111
display id: annotation2130111
version: 1
top level: YF1
location: https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
type: Range
persistent identity: range2130111
display id: range2130111
version: 1
top level: YF1
direction: direction/forward
start: 1
end: 1131
orientation: inline

FixJ
type: ComponentDefinition
persistent identity: BBa K592005
display id: BBa_K592005
version: 1
was generated by: iGEM to SBOL conversion
description: FixJ, the response regulator to YF1
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
mutable description: FixJ comes from the genome of Bradyrhizobium japonicum. It's the wild-type response regulator to YF1, the blue light-sensing fusion protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FixJ
sequence: https://synbiohub.org/public/igem/BBa_K592005_sequence/1

FixJ
type: SequenceAnnotation
persistent identity: annotation2130114
display id: annotation2130114
version: 1
top level: FixJ
location: https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
type: Range
persistent identity: range2130114
display id: range2130114
version: 1
top level: FixJ
direction: direction/forward
start: 1
end: 621
orientation: inline

https://synbiohub.org/public/igem/BBa_K1649003_sequence/1
type: Sequence
persistent identity: BBa K1649003 sequence
display id: BBa_K1649003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggccggagccgattatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13504_sequence/1
type: Sequence
persistent identity: BBa I13504 sequence
display id: BBa_I13504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592006_sequence/1
type: Sequence
persistent identity: BBa K592006 sequence
display id: BBa_K592006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592016_sequence/1
type: Sequence
persistent identity: BBa K592016 sequence
display id: BBa_K592016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823006_sequence/1
type: Sequence
persistent identity: BBa K823006 sequence
display id: BBa_K823006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592004_sequence/1
type: Sequence
persistent identity: BBa K592004 sequence
display id: BBa_K592004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592005_sequence/1
type: Sequence
persistent identity: BBa K592005 sequence
display id: BBa_K592005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z